 |
||||
| 2008 JASN IMPACT FACTOR 7.505 | HOME AUTHOR INFO EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP | |||
| CURRENT ISSUE | ARCHIVES | JASN Express | ONLINE SUBMISSION | |
|
||||
| 2008 JASN IMPACT FACTOR 7.505 | HOME AUTHOR INFO EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP | |||
| CURRENT ISSUE | ARCHIVES | JASN Express | ONLINE SUBMISSION | |
REGULAR ARTICLES |
*
Department of Pathology, Emory University School of Medicine, Atlanta,
Georgia.
Department of Physiology, Emory University School of Medicine, Atlanta,
Georgia.
Renal Division, Department of Medicine, Emory University School of
Medicine, Atlanta, Georgia.
Correspondence to Dr. Serena M. Bagnasco, Department of Pathology, Emory University School of Medicine, WMB Room 7105 C, 1639 Pierce Drive, Atlanta, GA 30322. Phone: 404-727-4026; Fax: 404-727-8540; E-mail: sbagnas{at}emory.edu
Abstract. Urea transport in the kidney is important for the production of concentrated urine and is mediated by a family of transporter proteins, identified from erythropoietic tissue (UT-B) and from kidney (UT-A). Two isoforms of the renal urea transporter (UT-A) have been cloned so far: UT-A1 and UT-A2. We used rapid amplification of cDNA ends to clone two new isoforms of the rat UT-A transporter: UT-A3 and UT-A4. UT-A3 and UT-A4 are 87% homologous. The UT-A3 cDNA encodes a peptide of 460 amino acids, which corresponds to the amino-terminal half of the UT-A1 peptide and is 62% identical to UT-A2. The UT-A4 cDNA encodes a peptide of 466 amino acids, which is 84% identical to UT-A2. Transient transfection of HEK-293 cells with the UT-A3 or UT-A4 cDNA results in phloretin-inhibitable urea uptake, which is increased by forskolin. Thus, both new isoforms encode functional urea transporters that may be vasopressin-regulated. UT-A3 and UT-A4 mRNA are expressed in the renal outer and inner medulla but not in the cortex; unidentified UT-A isoforms similar to UT-A3 may also be expressed in the testis. It is concluded that there are at least four different rat UT-A urea transporters.
This article has been cited by other articles:
 |
|
 |
|
  X. Feng, H. Huang, Y. Yang, O. Frohlich, J. D. Klein, J. M. Sands, and G. Chen Caveolin-1 directly interacts with UT-A1 urea transporter: the role of caveolae/lipid rafts in UT-A1 regulation at the cell membrane Am J Physiol Renal Physiol, June 1, 2009; 296(6): F1514 - F1520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Stewart, A. Thistlethwaite, H. Lees, G. J. Cooper, and C. Smith Vasopressin regulation of the renal UT-A3 urea transporter Am J Physiol Renal Physiol, March 1, 2009; 296(3): F642 - F648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Uchiyama, R. Kikuchi, N. Konno, T. Wakasugi, and K. Matsuda Localization and regulation of a facilitative urea transporter in the kidney of the red-eared slider turtle (Trachemys scripta elegans) J. Exp. Biol., January 15, 2009; 212(2): 249 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. W. Blessing, M. A. Blount, J. M. Sands, C. F. Martin, and J. D. Klein Urea transporters UT-A1 and UT-A3 accumulate in the plasma membrane in response to increased hypertonicity Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1336 - F1341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chen, H. Huang, O. Frohlich, Y. Yang, J. D. Klein, S. R. Price, and J. M. Sands MDM2 E3 ubiquitin ligase mediates UT-A1 urea transporter ubiquitination and degradation Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1528 - F1534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Castrop and J. Schnermann Isoforms of renal Na-K-2Cl cotransporter NKCC2: expression and functional significance Am J Physiol Renal Physiol, October 1, 2008; 295(4): F859 - F866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. MacIver, C. P. Smith, W. G. Hill, and M. L. Zeidel Functional characterization of mouse urea transporters UT-A2 and UT-A3 expressed in purified Xenopus laevis oocyte plasma membranes Am J Physiol Renal Physiol, April 1, 2008; 294(4): F956 - F964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Blount, J. D. Klein, C. F. Martin, D. Tchapyjnikov, and J. M. Sands Forskolin stimulates phosphorylation and membrane accumulation of UT-A3 Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1308 - F1313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Stewart, S. L. King, E. A. Potter, and C. P. Smith Acute regulation of mUT-A3 urea transporter expressed in a MDCK cell line Am J Physiol Renal Physiol, April 1, 2007; 292(4): F1157 - F1163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Fenton and M. A. Knepper Urea and Renal Function in the 21st Century: Insights from Knockout Mice J. Am. Soc. Nephrol., March 1, 2007; 18(3): 679 - 688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Frohlich, J. D. Klein, P. M. Smith, J. M. Sands, and R. B. Gunn Regulation of UT-A1-mediated transepithelial urea flux in MDCK cells Am J Physiol Cell Physiol, October 1, 2006; 291(4): C600 - C606. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Janech, W. R. Fitzgibbon, M. W. Nowak, D. H. Miller, R. V. Paul, and D. W. Ploth Cloning and functional characterization of a second urea transporter from the kidney of the Atlantic stingray, Dasyatis sabina Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2006; 291(3): R844 - R853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Potter, G. Stewart, and C. P. Smith Urea flux across MDCK-mUT-A2 monolayers is acutely sensitive to AVP, cAMP, and [Ca2+]i Am J Physiol Renal Physiol, July 1, 2006; 291(1): F122 - F128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Stewart, C. P. Smith, and G. J. Cooper Molecular characterization of the mercurial sensitivity of a frog urea transporter (fUT) Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1437 - F1442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Doran, J. D. Klein, Y. H. Kim, T. D. Smith, S. D. Kozlowski, R. B. Gunn, and J. M. Sands Tissue distribution of UT-A and UT-B mRNA and protein in rat Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1446 - R1459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Konno, S. Hyodo, K. Matsuda, and M. Uchiyama Effect of osmotic stress on expression of a putative facilitative urea transporter in the kidney and urinary bladder of the marine toad, Bufo marinus J. Exp. Biol., April 1, 2006; 209(7): 1207 - 1216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-W. Lim, K.-H. Han, J.-Y. Jung, W.-Y. Kim, C.-W. Yang, J. M. Sands, M. A. Knepper, K. M. Madsen, and J. Kim Ultrastructural localization of UT-A and UT-B in rat kidneys with different hydration status Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2006; 290(2): R479 - R492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Uchida, E. Sohara, T. Rai, M. Ikawa, M. Okabe, and S. Sasaki Impaired Urea Accumulation in the Inner Medulla of Mice Lacking the Urea Transporter UT-A2 Mol. Cell. Biol., August 15, 2005; 25(16): 7357 - 7363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Stewart, C. Graham, S. Cattell, T. P. L. Smith, N. L. Simmons, and C. P. Smith UT-B is expressed in bovine rumen: potential role in ruminal urea transport Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2005; 289(2): R605 - R612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Lucien, P. Bruneval, F. Lasbennes, M.-F. Belair, C. Mandet, J.-P. Cartron, P. Bailly, and M.-M. Trinh-Trang-Tan UT-B1 urea transporter is expressed along the urinary and gastrointestinal tracts of the mouse Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2005; 288(4): R1046 - R1056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Mistry, G. Chen, A. Kato, K. Nag, J. M. Sands, and S. Hirose A novel type of urea transporter, UT-C, is highly expressed in proximal tubule of seawater eel kidney Am J Physiol Renal Physiol, March 1, 2005; 288(3): F455 - F465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bonilla-Felix Development of water transport in the collecting duct Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1093 - F1101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. P. Smith, E. A. Potter, R. A. Fenton, and G. S. Stewart Characterization of a human colonic cDNA encoding a structurally novel urea transporter, hUT-A6 Am J Physiol Cell Physiol, October 1, 2004; 287(4): C1087 - C1093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Inoue, S. D. Jackson, T. Vikulina, J. D. Klein, K. Tomita, and S. M. Bagnasco Identification and characterization of a Kidd antigen/UT-B urea transporter expressed in human colon Am J Physiol Cell Physiol, July 1, 2004; 287(1): C30 - C35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Li, J. D. Klein, W. Wang, M. A. Knepper, S. Nielsen, J. M. Sands, and J. Frokiaer Altered expression of urea transporters in response to ureteral obstruction Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1154 - F1162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Fenton, C.-L. Chou, G. S. Stewart, C. P. Smith, and M. A. Knepper Urinary concentrating defect in mice with selective deletion of phloretin-sensitive urea transporters in the renal collecting duct PNAS, May 11, 2004; 101(19): 7469 - 7474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Klein, J. M. Sands, L. Qian, X. Wang, and B. Yang Upregulation of Urea Transporter UT-A2 and Water Channels AQP2 and AQP3 in Mice Lacking Urea Transporter UT-B J. Am. Soc. Nephrol., May 1, 2004; 15(5): 1161 - 1167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Stewart, R. A. Fenton, W. Wang, T.-H. Kwon, S. J. White, V. M. Collins, G. Cooper, S. Nielsen, and C. P. Smith The basolateral expression of mUT-A3 in the mouse kidney Am J Physiol Renal Physiol, May 1, 2004; 286(5): F979 - F987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-M. Trinh-Trang-Tan, G. Geelen, L. Teillet, and B. Corman Urea transporter expression in aging kidney and brain during dehydration Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2003; 285(6): R1355 - R1365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Morgan, J. S. Ballantyne, and P. A. Wright Regulation of a renal urea transporter with reduced salinity in a marine elasmobranch, Raja erinacea J. Exp. Biol., September 15, 2003; 206(18): 3285 - 3292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Bagnasco Gene structure of urea transporters Am J Physiol Renal Physiol, January 1, 2003; 284(1): F3 - F10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Sands Molecular Approaches to Urea Transporters J. Am. Soc. Nephrol., November 1, 2002; 13(11): 2795 - 2806. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Fenton, G. S. Stewart, B. Carpenter, A. Howorth, E. A. Potter, G. J. Cooper, and C. P. Smith Characterization of mouse urea transporters UT-A1 and UT-A2 Am J Physiol Renal Physiol, October 1, 2002; 283(4): F817 - F825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Janech, R. Chen, J. Klein, M. W. Nowak, W. McFee, R. V. Paul, W. R. Fitzgibbon, and D. W. Ploth Molecular and functional characterization of a urea transporter from the kidney of a short-finned pilot whale Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2002; 282(5): R1490 - R1500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Peng, J. M. Sands, and S. M. Bagnasco Glucocorticoids inhibit transcription and expression of the UT-A urea transporter gene Am J Physiol Renal Physiol, May 1, 2002; 282(5): F853 - F858. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yang, L. Bankir, A. Gillespie, C. J. Epstein, and A. S. Verkman Urea-selective Concentrating Defect in Transgenic Mice Lacking Urea Transporter UT-B J. Biol. Chem., March 15, 2002; 277(12): 10633 - 10637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Klein, P. Rouillard, B. R. Roberts, and J. M. Sands Acidosis Mediates the Upregulation of UT-A Protein in Livers from Uremic Rats J. Am. Soc. Nephrol., March 1, 2002; 13(3): 581 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Kim, D.-U. Kim, K.-H. Han, J.-Y. Jung, J. M. Sands, M. A. Knepper, K. M. Madsen, and J. Kim Expression of urea transporters in the developing rat kidney Am J Physiol Renal Physiol, March 1, 2002; 282(3): F530 - F540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Mistry, S. Honda, T. Hirata, A. Kato, and S. Hirose Eel urea transporter is localized to chloride cells and is salinity dependent Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2001; 281(5): R1594 - R1604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gamba Alternative splicing and diversity of renal transporters Am J Physiol Renal Physiol, November 1, 2001; 281(5): F781 - F794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Bagnasco, T. Peng, M. G. Janech, A. Karakashian, and J. M. Sands Cloning and characterization of the human urea transporter UT-A1 and mapping of the human Slc14a2 gene Am J Physiol Renal Physiol, September 1, 2001; 281(3): F400 - F406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Quigley, A. Lisec, and M. Baum Ontogeny of rabbit proximal tubule urea permeability Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2001; 280(6): R1713 - R1718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Shayakul, H. Tsukaguchi, U. V. Berger, and M. A. Hediger Molecular characterization of a novel urea transporter from kidney inner medullary collecting ducts Am J Physiol Renal Physiol, March 1, 2001; 280(3): F487 - F494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Terris, M. A. Knepper, and J. B. Wade UT-A3: localization and characterization of an additional urea transporter isoform in the IMCD Am J Physiol Renal Physiol, February 1, 2001; 280(2): F325 - F332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Walsh, M Grosell, G. Goss, H. Bergman, A. Bergman, P Wilson, P Laurent, S. Alper, C. Smith, C Kamunde, et al. Physiological and molecular characterization of urea transport by the gills of the Lake Magadi tilapia (Alcolapia grahami) J. Exp. Biol., January 2, 2001; 204(3): 509 - 520. [Abstract] [PDF]
|
|
|
|
|
|
R. A. Fenton, A. Howorth, G. J. Cooper, R. Meccariello, I. D. Morris, and C. P. Smith Molecular characterization of a novel UT-A urea transporter isoform (UT-A5) in testis Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1425 - C1431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. BAGNASCO, T. PENG, Y. NAKAYAMA, and J. M. SANDS Differential Expression of Individual UT-A Urea Transporter Isoforms in Rat Kidney J. Am. Soc. Nephrol., November 1, 2000; 11(11): 1980 - 1986. [Abstract] [Full Text]
|
|
|
|
|
|
P. Walsh, M. Heitz, C. Campbell, G. Cooper, M Medina, Y. Wang, G. Goss, V Vincek, C. Wood, and C. Smith Molecular characterization of a urea transporter in the gill of the gulf toadfish (Opsanus beta) J. Exp. Biol., January 8, 2000; 203(15): 2357 - 2364. [Abstract] [PDF]
|
|
|
|
|
|
M. McDonald, C. Wood, Y Wang, and P. Walsh Differential branchial and renal handling of urea, acetamide and thiourea in the gulf toadfish Opsanus beta: evidence for two transporters J. Exp. Biol., January 3, 2000; 203(6): 1027 - 1037. [Abstract] [PDF]
|
|
|
|
|
|
J. B. Wade, A. J. Lee, J. Liu, C. A. Ecelbarger, C. Mitchell, A. D. Bradford, J. Terris, G.-H. Kim, and M. A. Knepper UT-A2: a 55-kDa urea transporter in thin descending limb whose abundance is regulated by vasopressin Am J Physiol Renal Physiol, January 1, 2000; 278(1): F52 - F62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. KLEIN, R. T. TIMMER, P. ROUILLARD, J. L. BAILEY, and J. M. SANDS UT-A Urea Transporter Protein Expressed in Liver: Upregulation byUremia J. Am. Soc. Nephrol., October 1, 1999; 10(10): 2076 - 2083. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Nakayama, T. Peng, J. M. Sands, and S. M. Bagnasco The TonE/TonEBP Pathway Mediates Tonicity-responsive Regulation of UT-A Urea Transporter Expression J. Biol. Chem., December 1, 2000; 275(49): 38275 - 38280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Fenton, G. J. Cooper, I. D. Morris, and C. P. Smith Coordinated expression of UT-A and UT-B urea transporters in rat testis Am J Physiol Cell Physiol, June 1, 2002; 282(6): C1492 - C1501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhang, J. M. Sands, and J. D. Klein Vasopressin rapidly increases phosphorylation of UT-A1 urea transporter in rat IMCDs through PKA Am J Physiol Renal Physiol, January 1, 2002; 282(1): F85 - F90. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Combet, L. Teillet, G. Geelen, B. Pitrat, R. Gobin, S. Nielsen, M.-M. Trinh-Trang-Tan, B. Corman, and J.-M. Verbavatz Food restriction prevents age-related polyuria by vasopressin-dependent recruitment of aquaporin-2 Am J Physiol Renal Physiol, December 1, 2001; 281(6): F1123 - F1131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Y. Wang, K. Beutler, J. Nielsen, S. Nielsen, M. A. Knepper, and S. Masilamani Decreased abundance of collecting duct urea transporters UT-A1 and UT-A3 with ECF volume expansion Am J Physiol Renal Physiol, April 1, 2002; 282(4): F577 - F584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Fenton, C. A. Cottingham, G. S. Stewart, A. Howorth, J. A. Hewitt, and C. P. Smith Structure and characterization of the mouse UT-A gene (Slc14a2) Am J Physiol Renal Physiol, April 1, 2002; 282(4): F630 - F638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Duchesne, J. D. Klein, J. B. Velotta, J. J. Doran, P. Rouillard, B. R. Roberts, A. A. McDonough, and J. M. Sands UT-A Urea Transporter Protein in Heart: Increased Abundance During Uremia, Hypertension, and Heart Failure Circ. Res., July 20, 2001; 89(2): 139 - 145. [Abstract] [Full Text] [PDF]
|
|
|
HOME
CURRENT ISSUE
ARCHIVES
JASN Express
ONLINE SUBMISSION
AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP |
Copyright © 2009 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673
