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Immunolocalization of Protein Kinase C Isoenzymes , ßI and ßII in Rat Kidney

IMKE L. PFAFF^*, HANS-JOACHIM WAGNER and VOLKER VALLON^*

^* Department of Pharmacology University of Tübingen, Tübingen, Germany
Department of Anatomy, University of Tübingen, Tübingen, Germany

Correspondence to Dr. Volker Vallon, Department of Pharmacology, University of Tübingen, Wilhelmstrasse 56, 72074 Tübingen, Germany. Phone: +49 07071 2972271; Fax: +49 07071 294942; E-mail: volker.vallon{at}uni-tuebingen.de

Abstract

Abstract. Protein kinase C (PKC) significantly contributes to the control of renal function, but little is known about the renal function or localization of PKC isoenzymes. Therefore, the localization of PKC isoenzymes , ßI, and ßII was studied in rat kidney. Immunoblot analysis identified immunoreactive bands corresponding to PKC , ßI, and ßII in total cell extracts of both renal cortex and medulla. Immunohistochemistry using confocal laser scanning microscopy revealed immunostaining for PKC within the glomerulus including podocytes and mesangial cells. PKC ßI was detected in mesangial cells, whereas anti-PKC ßII labeled neither podocytes nor mesangial cells. PKC ßII, however, was detected in cells within the mesangial area, which expressed MHC II, a marker for antigen-presenting cells. None of the three isoforms was detected in glomerular endothelial cells. A prominent immunostaining with anti-PKC and ßI was localized to the brush border of S2 and S3 segments of proximal tubule, whereas S1 segments were not stained. Along the loop of Henle, both PKC and PKC ßI were found in the luminal membrane of cortical and medullary thick ascending limb. In addition, anti-PKC ßI labeled the luminal membrane of thin limbs. In the cortical collecting duct (CCD), immunofluorescence for PKC was observed at the apical membrane of both peanut agglutinin (PNA)-negative cells and part of PNA-positive cells, whereas in the medullary collecting duct (MCD), PKC was detected at the basolateral membrane. In comparison, PKC ßI was localized at the luminal membrane of PNA-positive cells only in CCD and at the luminal membrane of MCD. Unlike PKC or ßI, there was (1) no detectable immunostaining with anti-PKC ßII in the proximal tubule, the loop of Henle, or the CCD and (2) a distinct staining for PKC ßII of interstitial cells in cortex and medulla (including MHC II-positive dendritic cells). Furthermore, PKC ßII was detected in the luminal membrane of MCD. In summary, a distinct and differential expression pattern for PKC , ßI, and ßII was shown in rat kidney, which may contribute to a better understanding of the specific role of these isoenzymes in the control of renal function.

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