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J Am Soc Nephrol 11:1848-1856, 2000
© 2000 American Society of Nephrology

Expression of Endothelial Nitric Oxide Synthase in Human Peritoneal Tissue

Regulation by Escherichia Coli Lipopolysaccharide

MARÍA M. ARRIERO*, JUAN A. RODRÍGUEZ-FEO*, ÁNGEL CELDRÁN*, LOURDES SÁNCHEZ DE MIGUEL*, FERNANDO GONZÁLEZ-FERNÁNDEZ*, JOSÉ FORTES{dagger}, ANA REYERO*, OCTAVIO FRIEYRO*, JUAN C. DE LA PINTA*, ANGELES FRANCO*, CARLOS PASTOR*, SANTOS CASADO* and ANTONIO LÓPEZ-FARRÉ*

* Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
{dagger} Department of Pathology, Fundación, Jiménez Díaz, Madrid, Spain.

Correspondence to Dr. Antonio López-Farré, Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Av Reyes Católicos, 2, Madrid 28040, Spain. Phone: 91-5504821; Fax: 91-5494764; E-mail: ALopeza{at}fjd.es

Abstract. Changes in the expression of endothelial nitric oxide synthase (eNOS) in the peritoneum could be involved in the peritoneal dysfunction associated with peritoneal inflammation. Demonstrated recently in bovine endothelial cells was the existence of cytosolic proteins that bind to the 3'-untranslated region (3'-UTR) of eNOS mRNA and could be implicated in eNOS mRNA stabilization. The present work demonstrates that eNOS protein is expressed in human endothelial and mesothelial peritoneal cells. Escherichia coli lipopolysaccharide shortened the half-life of eNOS message, reducing eNOS protein expression in peritoneal mesothelial and endothelial cells. Moreover, under basal conditions, human peritoneal samples expressed cytosolic proteins that bind to the 3'-UTR of eNOS mRNA. The cytosolic proteins that directly bind to 3'-UTR were identified as a 60-kD protein. After incubation of human peritoneal samples with lipopolysaccharide, the binding activity of the cytosolic 60-kD protein increased in a time-dependent manner. Studies are now necessary to determine the involvement of this 60-kD protein in the regulation of eNOS expression in peritoneal cells and particularly its involvement in the peritoneal dysfunction associated with inflammatory reactions.




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