 |
||||
| 2007 JASN IMPACT FACTOR 7.111 | HOME AUTHOR INFO EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP | |||
| CURRENT ISSUE | ARCHIVES | JASN Express | ONLINE SUBMISSION | |
|
||||
| 2007 JASN IMPACT FACTOR 7.111 | HOME AUTHOR INFO EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP | |||
| CURRENT ISSUE | ARCHIVES | JASN Express | ONLINE SUBMISSION | |
,§
,§
*
Department of Bacteriology and Immunology, Haartman Institute, University
of Helsinki, Helsinki, Finland.
Department of Pathology, Haartman Institute, University of Helsinki,
Helsinki, Finland.
Transplantation Laboratory, Haartman Institute, University of Helsinki,
Helsinki, Finland.
§
Helsinki University Central Hospital Laboratory Diagnostic, Helsinki,
Finland.
Correspondence to Dr. Risto Renkonen, Haartmaninkatu 3, The Haartman Institute, University of Helsinki, Department of Bacteriology and Immunology, P.O. Box 21, FIN-00014 Helsinki, Finland; Phone: +358-9-1912-6387; Fax: +358-9-1912-6382; E-mail: risto.renkonen{at}helsinki.fi
Abstract. Acute kidney allograft rejection is characterized by a lymphocyte infiltration. L-selectin on lymphocytes and its endothelial glycosylated ligands are instrumental in the initiation of lymphocyte extravasation to sites of inflammation. From more than 500 core biopsy specimens taken from kidneys after transplantation, 250 biopsies were graded to have signs of acute rejection. Of these, 52 biopsies with various grades of histologic signs of acute rejection were selected for the study. Controls were 15 biopsies taken within 30 min after revascularization and 10 specimens from well-functioning allografts showing no clinical or histologic evidence of rejection. Immunochemical stainings with monoclonal antibodies against functionally active decorated L-selectin ligands. i.e., sialyl-Lewis x (sLex, 2F3 and HECA-452) or sulfated lactosamine (MECA-79) were performed. Although no endothelial 2F3 and MECA-79 epitopes were detected in nonrejecting control specimens, the expression was induced at the onset and during acute allograft rejections. The level of expression (in semi-quantitative score) of 2F3 reactivity correlated with the severity of rejection (P < 0.0001, grade I versus grade IIB), and the level of expression decreased as the rejection resolved. Kidney biopsies taken shortly after revascularization and thus undergoing reperfusion injury showed endothelial staining with another anti sLex antibody, HECA-452. This staining disappeared from well-functioning grafts and reappeared at the onset of an acute allograft rejection. These results suggest that expression of functionally active, properly glycosylated L-selectin ligands might have a role in reperfusion injury and in the initiation of acute rejections after human kidney allograft transplantation.
This article has been cited by other articles:
 |
|
 |
|
  A. Izawa, T. Ueno, M. Jurewicz, T. Ito, K. Tanaka, M. Takahashi, U. Ikeda, O. Sobolev, P. Fiorina, R. N. Smith, et al. Importance of Donor- and Recipient-Derived Selectins in Cardiac Allograft Rejection J. Am. Soc. Nephrol., November 1, 2007; 18(11): 2929 - 2936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W.A.M. Celie, N. W.P. Rutjes, E. D. Keuning, R. Soininen, R. Heljasvaara, T. Pihlajaniemi, A. M. Drager, S. Zweegman, F. L. Kessler, R. H.J. Beelen, et al. Subendothelial Heparan Sulfate Proteoglycans Become Major L-Selectin and Monocyte Chemoattractant Protein-1 Ligands upon Renal Ischemia/Reperfusion Am. J. Pathol., June 1, 2007; 170(6): 1865 - 1878. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Toppila-Salmi, J. P. Myller, T. V. M. Torkkeli, J. V. Muhonen, J. A. Renkonen, M. E. Rautiainen, and R. L. O. Renkonen Endothelial L-Selectin Ligands in Sinus Mucosa during Chronic Maxillary Rhinosinusitis Am. J. Respir. Crit. Care Med., June 15, 2005; 171(12): 1350 - 1357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rivera-Nieves, T. Olson, G. Bamias, A. Bruce, M. Solga, R. F. Knight, S. Hoang, F. Cominelli, and K. Ley L-Selectin, {alpha}4{beta}1, and {alpha}4{beta}7 Integrins Participate in CD4+ T Cell Recruitment to Chronically Inflamed Small Intestine J. Immunol., February 15, 2005; 174(4): 2343 - 2352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Huopaniemi, M. Kolmer, J. Niittymaki, M. Pelto-Huikko, and R. Renkonen Inflammation-induced transcriptional regulation of Golgi transporters required for the synthesis of sulfo sLex glycan epitopes Glycobiology, December 1, 2004; 14(12): 1285 - 1294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bistrup, D. Tsay, P. Shenoy, M. S. Singer, N. Bangia, S. A. Luther, J. G. Cyster, N. H. Ruddle, and S. D. Rosen Detection of a Sulfotransferase (HEC-GlcNAc6ST) in High Endothelial Venules of Lymph Nodes and in High Endothelial Venule-Like Vessels within Ectopic Lymphoid Aggregates: Relationship to the MECA-79 Epitope Am. J. Pathol., May 1, 2004; 164(5): 1635 - 1644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maki and R. Renkonen Biosynthesis of 6-deoxyhexose glycans in bacteria Glycobiology, March 1, 2004; 14(3): 1R - 15R. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Ogawa, K. Shikata, K. Honke, S. Sato, M. Matsuda, R. Nagase, A. Tone, S. Okada, H. Usui, J. Wada, et al. Cerebroside Sulfotransferase Deficiency Ameliorates L-selectin-dependent Monocyte Infiltration in the Kidney after Ureteral Obstruction J. Biol. Chem., January 16, 2004; 279(3): 2085 - 2090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Renkonen, O. Tynninen, P. Hayry, T. Paavonen, and R. Renkonen Glycosylation Might Provide Endothelial Zip Codes for Organ-Specific Leukocyte Traffic into Inflammatory Sites Am. J. Pathol., August 1, 2002; 161(2): 543 - 550. [Abstract] [Full Text] [PDF]
|
|
|
HOME
CURRENT ISSUE
ARCHIVES
JASN Express
ONLINE SUBMISSION
AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP |
Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673
