2007 JASN IMPACT FACTOR 7.111	HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP
advanced	CURRENT ISSUE	ARCHIVES	JASN Express	ONLINE SUBMISSION

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by SMOYER, W. E. Articles by BENNDORF, R. Search for Related Content PubMed PubMed Citation Articles by SMOYER, W. E. Articles by BENNDORF, R.

Ischemic Acute Renal Failure Induces Differential Expression of Small Heat Shock Proteins

WILLIAM E. SMOYER^*, RICHARD RANSOM^*, RAYMOND C. HARRIS, MICHAEL J. WELSH, GUDRUN LUTSCH^§ and RAINER BENNDORF

^* Department of Pediatrics University of Michigan, Ann Arbor, Michigan
Department of Medicine, Vanderbilt University, Nashville, Tennessee
Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan
^§ Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany.

Correspondence to Dr. William E. Smoyer, Pediatric Nephrology, University of Michigan Medical Center, 8220E MSRB III, Box 0646, 1150 W. Medical Center Drive, Ann Arbor, MI 48109. Phone: 734-763-9524; Fax: 734-615-1386; E-mail: wsmoyer{at}umich.edu

B-crystallin and heat shock protein (hsp) 25 are structurally and functionally related small stress proteins induced by a variety of insults, including heat and ischemia. Cytoprotection by these two hsp is thought to result from molecular chaperoning and/or cytoskeletal stabilization. Because renal ischemia is characterized by disruption of the renal tubular cell actin cytoskeleton, this study was conducted to determine the localization and quantify the expression and phosphorylation of both hsp in renal cortex, isolated glomeruli, outer medulla, and inner medulla of rats after bilateral renal ischemia. Sham-operated kidneys had similarly small amounts of hsp25 and B-crystallin in cortex and glomeruli, with substantially greater amounts of B-crystallin versus hsp25 in outer and inner medulla. Ischemia resulted in significantly increased hsp25 (and hsp70i) but variable B-crystallin levels in cortex and outer medulla, and progressively decreased glomerular hsp25 phosphorylation. In sham-operated kidneys, hsp25 localized to glomeruli, vessels, and collecting ducts, with B-crystallin primarily in medullary thin limbs and collecting ducts. After ischemia, hsp25 accumulated in proximal tubules in cortex and outer medulla, while B-crystallin labeling became nonhomogeneous in outer medulla, and increased in Bowman's capsule. It is concluded that: (1) There is striking differential expression of hsp25 and B-crystallin in various renal compartments; and (2) Renal ischemia results in differential accumulation of hsp25 and B-crystallin, with hsp25 part of a generalized stress response in renal proximal tubular cells, which may play a role in recovery from ischemia-induced actin filament disruption.

This article has been cited by other articles:

				J.-K. Park, N. Ronkina, A. Hoft, C. Prohl, J. Menne, M. Gaestel, H. Haller, and M. Meier Deletion of MK2 signalling in vivo inhibits small Hsp phosphorylation but not diabetic nephropathy Nephrol. Dial. Transplant., June 1, 2008; 23(6): 1844 - 1853. [Abstract] [Full Text] [PDF]

				M. C. Korrapati, J. Chilakapati, F. A. Witzmann, C. Rao, E. A. Lock, and H. M. Mehendale Proteomics of S-(1, 2-dichlorovinyl)-L-cysteine-induced acute renal failure and autoprotection in mice Am J Physiol Renal Physiol, October 1, 2007; 293(4): F994 - F1006. [Abstract] [Full Text] [PDF]

				A. V. Cybulsky, T. Takano, J. Papillon, and K. Bijian Role of the Endoplasmic Reticulum Unfolded Protein Response in Glomerular Epithelial Cell Injury J. Biol. Chem., July 1, 2005; 280(26): 24396 - 24403. [Abstract] [Full Text] [PDF]

				S. Hirano, X. Sun, C. A. DeGuzman, R. F. Ransom, K. R. McLeish, W. E. Smoyer, E. A. Shelden, M. J. Welsh, and R. Benndorf p38 MAPK/HSP25 signaling mediates cadmium-induced contraction of mesangial cells and renal glomeruli Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1133 - F1143. [Abstract] [Full Text] [PDF]

				D. P. Basile, K. Fredrich, M. Alausa, C. P. Vio, M. Liang, M. R. Rieder, A. S. Greene, and A. W. Cowley Jr. Identification of persistently altered gene expression in the kidney after functional recovery from ischemic acute renal failure Am J Physiol Renal Physiol, May 1, 2005; 288(5): F953 - F963. [Abstract] [Full Text] [PDF]

				T. E. Eichler, R. F. Ransom, and W. E. Smoyer Differential Induction of Podocyte Heat Shock Proteins by Prolonged Single and Combination Toxic Metal Exposure Toxicol. Sci., March 1, 2005; 84(1): 120 - 128. [Abstract] [Full Text] [PDF]

				T. F. Mueller, C. Ma, J. A. Lederer, and D. L. Perkins Differentiation of stress, metabolism, communication, and defense responses following transplantation J. Leukoc. Biol., March 1, 2003; 73(3): 379 - 390. [Abstract] [Full Text] [PDF]

				B. C. Santos, J. M. Pullman, A. Chevaile, W. J. Welch, and S. R. Gullans Chronic hyperosmolarity mediates constitutive expression of molecular chaperones and resistance to injury Am J Physiol Renal Physiol, March 1, 2003; 284(3): F564 - F574. [Abstract] [Full Text] [PDF]

				S. K. Van Why, A. S. Mann, T. Ardito, G. Thulin, S. Ferris, M. A. Macleod, M. Kashgarian, and N. J. Siegel Hsp27 Associates with Actin and Limits Injury in Energy Depleted Renal Epithelia J. Am. Soc. Nephrol., January 1, 2003; 14(1): 98 - 106. [Abstract] [Full Text] [PDF]

				S. Bapat, J. A. Post, B. Braam, R. Goldschmeding, H. A. Koomans, A. J. Verkleij, and J. A. Joles Visualizing Tubular Lipid Peroxidation in Intact Renal Tissue in Hypertensive Rats J. Am. Soc. Nephrol., December 1, 2002; 13(12): 2990 - 2996. [Abstract] [Full Text] [PDF]

				E. A. Shelden, M. J. Borrelli, F. M. Pollock, and R. Bonham Heat Shock Protein 27 Associates with Basolateral Cell Boundaries in Heat-Shocked and ATP-Depleted Epithelial Cells J. Am. Soc. Nephrol., February 1, 2002; 13(2): 332 - 341. [Abstract] [Full Text] [PDF]

				N. Ishizaka, T. Aizawa, M. Ohno, S.-i. Usui, I. Mori, S.-S. Tang, J. R. Ingelfinger, S. Kimura, and R. Nagai Regulation and Localization of HSP70 and HSP25 in the Kidney of Rats Undergoing Long-Term Administration of Angiotensin II Hypertension, January 1, 2002; 39(1): 122 - 128. [Abstract] [Full Text] [PDF]

				K. M. Park, A. Chen, and J. V. Bonventre Prevention of Kidney Ischemia/Reperfusion-induced Functional Injury and JNK, p38, and MAPK Kinase Activation by Remote Ischemic Pretreatment J. Biol. Chem., April 6, 2001; 276(15): 11870 - 11876. [Abstract] [Full Text] [PDF]

				K. M. Park, C. Kramers, M. Vayssier-Taussat, A. Chen, and J. V. Bonventre Prevention of Kidney Ischemia/Reperfusion-induced Functional Injury, MAPK and MAPK Kinase Activation, and Inflammation by Remote Transient Ureteral Obstruction J. Biol. Chem., January 11, 2002; 277(3): 2040 - 2049. [Abstract] [Full Text] [PDF]

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673