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Nephrocystin

Gene Expression and Sequence Conservation between Human, Mouse, and Caenorhabditis Elegans

EDGAR OTTO^*, ANDREAS KISPERT, SILVIA SCHÄTZLE^*, BIRGIT LESCHER, CORNELIA RENSING^* and FRIEDHELM HILDEBRANDT^*

^* University Children's Hospital, Freiburg University, Freiburg, Germany.
Max-Planck Institute for Immunobiology, Freiburg, Germany.

Correspondence to Dr. Friedhelm Hildebrandt, University Children's Hospital, Mathildenstrasse 1, D-79106, Freiburg, Germany. Phone: +49 761 270 4301; Fax: +49 761 270 4533; E-mail: hildebra{at}kk1200.ukl.uni-freiburg.de

Juvenile nephronophthisis, an autosomal recessive cystic kidney disease, is the primary genetic cause for chronic renal failure in children. The gene (NPHP 1) for nephronophthisis type 1 has recently been identified. Its gene product, nephrocystin, is a novel protein of unknown function, which contains a src-homology 3 domain. To study tissue expression and analyze amino acid sequence conservation of nephrocystin, the full-length murine Nphp 1 cDNA sequence was obtained and Northern and in situ hybridization analyses were performed for extensive expression studies. The results demonstrate widespread but relatively weak NPHP 1 expression in the human adult. In the adult mouse there is strong expression in testis. This expression occurs specifically in cell stages of the first meiotic division and thereafter. In situ hybridization to whole mouse embryos demonstrated widespread and uniform expression at all developmental stages. Amino acid sequence conservation studies in human, mouse, and Caenorhabditis elegans show that in nephrocystin the src-homology 3 domain is embedded in a novel context of other putative domains of protein-protein interaction, such as coiled-coil and E-rich domains. It is concluded that for multiple putative protein-protein interaction domains of nephrocystin, sequence conservation dates back at least to Caenorhabditis elegans. The previously described discrepancy between widespread tissue expression and the restriction of symptoms to the kidney has now been confirmed by an in-depth expression study.

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