Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LEHRKE, I.
Right arrow Articles by WAGNER, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LEHRKE, I.
Right arrow Articles by WAGNER, J.
J Am Soc Nephrol 12:2321-2329, 2001
© 2001 American Society of Nephrology

Renal Endothelin-1 and Endothelin Receptor Type B Expression in Glomerular Diseases with Proteinuria

INGO LEHRKE, RÜDIGER WALDHERR, EBERHARD RITZ and JÜRGEN WAGNER

Department of Nephrology, University of Heidelberg, Germany.

Correspondence to Dr. Jürgen Wagner, Department of Nephrology, University Hospital, University of Heidelberg, Bergheimerstrasse 56a, D-69115 Heidelberg, Germany. Phone: +49-6221-911210; Fax: +49-6221-911279; E-mail: Juergen_wagner{at}med.uni-heidelberg.de

Abstract. The endothelin (ET) system has been studied extensively in experimental models of progressive chronic renal disease, but there is limited information regarding the ET system in renal patients. First, the expression of human ET-1, as well as ET receptor type A (ET-RA) and ET-RB, was studied in 26 renal biopsies from patients with different renal diseases. Gene expression was assessed by quantitative reverse transcription-PCR. Second, ET-1 and ET-RB protein expression and localization were examined, by immunohistochemical analyses, among a homogeneous cohort of 16 patients with IgA nephropathy and different degrees of proteinuria. ET-RB mRNA expression was threefold higher among patients with higher-grade proteinuria [>=2 g/24 h, n = 10; OD ratio (ODR), i.e., wild-type/mutant mRNA ratio, 1.81 ± 0.3], compared with patients with lower-grade proteinuria (<2 g/24 h, n = 8; ODR, 0.63 ± 0.1; P < 0.01) or control subjects (n = 9; ODR, 0.57 ± 0.1; P < 0.01). ET-1 gene expression was significantly higher among patients with higher-grade proteinuria, compared with patients with lower-grade proteinuria (P < 0.01) or control subjects (P < 0.05). ET-RA mRNA expression was not different among the groups. Patients with higher-grade proteinuria who were receiving angiotensin-converting enzyme inhibitors exhibited significantly (P < 0.05) lower ET-1 and ET-RB mRNA expression, which was comparable to that of control subjects. By using immunohistochemical analyses, an association between proteinuria and expression of ET-1 and ET-RB in proximal tubular epithelial cells and of ET-1 in glomeruli was confirmed in the separate cohort of patients with IgA nephropathy. It is concluded that the increased ET-RB and ET-1 mRNA and protein expression observed in animal models of renal disease is also demonstrable among patients with renal disease and high-grade proteinuria.




This article has been cited by other articles:


Home page
 Rheumatology (Oxford)Home page
K. W. Frommer and U. Muller-Ladner
Expression and function of ETA and ETB receptors in SSc
Rheumatology, October 1, 2008; 47(suppl_5): v27 - v28.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
W. Neuhofer and D. Pittrow
Endothelin in human autoimmune diseases with renal involvement
Rheumatology, October 1, 2006; 45(suppl_3): iii39 - iii41.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
K. Frank, M. Zeier, M.-L. Gross, R. Waldherr, E. Ritz, and K. Amann
Comprehensive immunohistological analysis of the endothelin system in human kidney grafts
Nephrol. Dial. Transplant., May 1, 2006; 21(5): 1365 - 1372.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
N. Dhaun, J. Goddard, and DavidJ. Webb
The Endothelin System and Its Antagonism in Chronic Kidney Disease
J. Am. Soc. Nephrol., April 1, 2006; 17(4): 943 - 955.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Moridaira, J. Morrissey, M. Fitzgerald, G. Guo, R. McCracken, T. Tolley, and S. Klahr
ACE inhibition increases expression of the ETB receptor in kidneys of mice with unilateral obstruction
Am J Physiol Renal Physiol, January 1, 2003; 284(1): F209 - F217.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
I. Lehrke, M. Schaier, K. Schade, C. Morath, R. Waldherr, E. Ritz, and J. Wagner
Retinoid receptor-specific agonists alleviate experimental glomerulonephritis
Am J Physiol Renal Physiol, April 1, 2002; 282(4): F741 - F751.
[Abstract] [Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673