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J Am Soc Nephrol 12:207-217, 2001
© 2001 American Society of Nephrology

Increased Abundance of Distal Sodium Transporters in Rat Kidney during Vasopressin Escape

CAROLYN A. ECELBARGER*, MARK A. KNEPPER{dagger} and JOSEPH G. VERBALIS*

* Department of Medicine, Division of Endocrinology and Metabolism, Georgetown University, Washington, D. C.
{dagger} Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland.

Correspondence to Dr. Carolyn A. Ecelbarger, Bulding D, Room 232, Georgetown University, 4000 Reservoir Road NW, Washington, DC. Phone: 202-687-0453; Fax: 202-687-2040; E-mail: ecelbarc{at}gunet.georgetown.edu

Abstract. Hyponatremia is associated with inappropriately elevated vasopressin levels. A brisk natriuresis precedes the escape from this antidiuresis. Thus, the hypothesis was that the abundance of one or more of the sodium transporters of the distal tubule (a site for fine tuning of sodium balance) would be altered during vasopressin escape. Semiquantitative immunoblotting was used to examine the regulation of abundance of several sodium transporters/channels of the thick ascending limb through the collecting duct in the rat model. Osmotic minipumps to infuse dDAVP, the V2-selective vasopressin agonist (5 ng/h) for the entire experiment, were implanted in Male Sprague-Dawley rats. After 4 d, rats were divided into a control (dry AIN-76 diet/ad libitum water) or a water-loaded (gelled-agar-AIN-76 diet/ad libitum water) group. Rats were killed after 1, 2, 3, or 7 additional days. The water-loaded rats were hyponatremic (plasma Na+, 98 to 122 mmol/L) and manifested the expected early natriuresis and diuresis of vasopressin escape. Water loading (with dDAVP infusion) resulted in increased whole-kidney abundances of the thiazide-sensitive Na-Cl co-transporter, the {alpha}-subunit of the epithelial sodium channel (ENaC), and the 70-kD dimer of the {gamma}-subunit of ENaC. No changes were observed for the ß-subunit of ENaC. Similar protein changes have recently been associated with elevated aldosterone levels in rats. However, plasma aldosterone levels were significantly suppressed in this model. These data suggest that several distal sodium reabsorptive mechanisms are upregulated during vasopressin escape; this may help to attenuate the developing hyponatremia resulting from water loading when vasopressin levels are inappropriately elevated.




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