Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by YANG, B.
Right arrow Articles by NAHAS, A. M. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by YANG, B.
Right arrow Articles by NAHAS, A. M. E.
J Am Soc Nephrol 12:275-288, 2001
© 2001 American Society of Nephrology

Expression of Apoptosis-Related Genes and Proteins in Experimental Chronic Renal Scarring

BIN YANG*,{dagger}, TIMOTHY S. JOHNSON*, GRAHAM L. THOMAS*,{dagger}, PHILIP F. WATSON{dagger}, BART WAGNER{ddagger}, NICHOLAS J. SKILL*, JOHN L. HAYLOR*,{dagger} and A. MEGUID EL NAHAS*,{dagger}

* Sheffield Kidney Institute, Northern General Hospital Trust, Sheffield, United Kingdom.
{dagger} Sheffield University Division of Clinical Sciences, Northern General Hospital Trust, Sheffield, United Kingdom.
{ddagger} Department of Histopathology, Northern General Hospital Trust, Sheffield, United Kingdom.

Correspondence to Professor A. Meguid El Nahas, Sheffield Kidney Institute, Northern General Hospital Trust, Herries Road, Sheffield S5 7AU, United Kingdom. Phone: +44-114-2714017; Fax: +44-114-2562514; E-mail: M.El-Nahas{at}sheffield.ac.uk

Abstract. Apoptosis has been proposed to play an important role in the progression of renal scarring. The mechanisms that determine whether a cell enters the apoptotic program are complex. Bax and Bcl-2 are recognized modulators of this event; their relative levels determine the fate of cells. A role for apoptosis in the progression of renal scarring in the remnant kidneys of rats submitted to subtotal nephrectomy (SNx) has been described. This study investigated the expression (protein and mRNA) of Bax and Bcl-2 in remnant kidneys between day 7 and day 120 post-SNx. Northern blot analysis showed that bax mRNA was increased in remnant kidneys from day 7 (150% of control; P < 0.05), whereas bcl-2 mRNA was decreased from day 15 (23% of control; P < 0.05) resulting in a 14-fold increase in the ratio of bax to bcl-2 mRNA by day 120. Western blot analysis showed similar changes in Bax and Bcl-2 protein in remnant kidneys, resulting in a 147-fold increase in the ratio of Bax to Bcl-2 on day 120. Immunohistochemistry showed increases in Bax to be located predominantly in tubules in SNx kidneys. Interstingly, Bcl-2 immunostaining increased in some epithelial cells within atrophic tubules despite the overall decrease in Bcl-2 protein and mRNA. The overall renal apoptotic cells correlated closely with the ratio of bax to bcl-2 at both the mRNA and protein levels (r = 0.594 and 0.308, respectively; P < 0.05). Furthermore, tubular apoptosis correlated positively with the mRNA level of bax (r = 0.471; P < 0.01) and negatively with the mRNA and protein levels of bcl-2 (r = -0.443 and -0.607, respectively; P < 0.01). The increase in the ratio of the death inducer (Bax) to the death repressor (Bcl-2) at the mRNA and protein levels may control the apoptosis associated with the progression of tubular atrophy and chronic renal fibrosis within remnant rat kidneys. These observations may have prognostic and therapeutic implications in chronic renal failure.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Mao, Z. Li, Y. Zhou, Z. Li, S. Zhuang, X. An, B. Zhang, W. Chen, J. Nie, Z. Wang, et al.
HSP72 attenuates renal tubular cell apoptosis and interstitial fibrosis in obstructive nephropathy
Am J Physiol Renal Physiol, July 1, 2008; 295(1): F202 - F214.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
C.-L. Lin, J.-Y. Wang, Y.-T. Huang, Y.-H. Kuo, K. Surendran, and F.-S. Wang
Wnt/beta-Catenin Signaling Modulates Survival of High Glucose-Stressed Mesangial Cells
J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2812 - 2820.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
T. S. Johnson, A. F. El-Koraie, N. J. Skill, N. M. Baddour, A. M. El Nahas, M. Njloma, A. G. Adam, and M. Griffin
Tissue Transglutaminase and the Progression of Human Renal Scarring
J. Am. Soc. Nephrol., August 1, 2003; 14(8): 2052 - 2062.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. Rossert, B. Fouqueray, and J. J. Boffa
Anemia Management and the Delay of Chronic Renal Failure Progression
J. Am. Soc. Nephrol., July 1, 2003; 14(90002): S173 - 177.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
J. Rossert, W. M. McClellan, S. D. Roger, and D. L. Verbeelen
Epoetin treatment: what are the arguments to expect a beneficial effect on renal disease progression?
Nephrol. Dial. Transplant., March 1, 2002; 17(3): 359 - 362.
[Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. Benigni, E. Gagliardini, A. Remuzzi, D. Corna, and G. Remuzzi
Angiotensin-Converting Enzyme nhibition Prevents Glomerular-Tubule Disconnection and Atrophy in Passive Heymann Nephritis, an Effect Not Observed with a Calcium Antagonist
Am. J. Pathol., November 1, 2001; 159(5): 1743 - 1750.
[Abstract] [Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673