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J Am Soc Nephrol 13:630-638, 2002
© 2002 American Society of Nephrology

A Conditionally Immortalized Human Podocyte Cell Line Demonstrating Nephrin and Podocin Expression

Moin A. Saleem*, Michael J. O’Hare{dagger}, Jochen Reiser{ddagger}, Richard J. Coward*, Carol D. Inward*, Timothy Farren*, Chang Ying Xing*, Lan Ni*, Peter W. Mathieson* and Peter Mundel{ddagger}

*Children’s Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, UK; {dagger}LICR/UCL Breast Cancer Laboratory, London, UK; {ddagger}Department of Medicine and Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York.

Correspondence to: Dr. Moin A. Saleem, Children’s Renal Unit and Academic Renal Unit, University of Bristol, Southmead Hospital, Bristol, BS10 5NB UK. Phone: +44-117-959-6048; Fax: +44-117-959-5438; E-mail: M.Saleem{at}bristol.ac.uk

ABSTRACT. Recent molecular insights have established the podocyte as a key component of the glomerular filtration barrier, and hence an important common pathway in proteinuric diseases. A conditionally immortalized human podocyte cell line has been developed by transfection with the temperature-sensitive SV40-T gene. These cells proliferate at the "permissive" temperature (33°C). After transfer to the "nonpermissive" temperature (37°C), they entered growth arrest and expressed markers of differentiated in vivo podocytes, including the novel podocyte proteins, nephrin, podocin, CD2AP, and synaptopodin, and known molecules of the slit diaphragm ZO-1, {alpha}-, ß-, and {gamma}-catenin and P-cadherin. The differentiation was accompanied by a growth arrest and the upregulation of cyclin-dependent kinase inhibitors, p27 and p57, as well as cyclin D1, whereas cyclin A was downregulated. These data are consistent with cell cycle protein expression during podocyte maturation in vivo. In conclusion, the development of this cell line provides a new tool in the study of podocyte biology, which will enable accurate assessment of the behavior of these complex cells in health and disease.




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