Journal of the American Society of Nephrology
2008 JASN IMPACT FACTOR 7.505 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eremina, V.
Right arrow Articles by Quaggin, S. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eremina, V.
Right arrow Articles by Quaggin, S. E.
J Am Soc Nephrol 13:788-793, 2002
© 2002 American Society of Nephrology

BRIEF COMMUNICATIONS

Glomerular-Specific Gene Excision In Vivo

Vera Eremina*, Mark Andrew Wong*, Shiying Cui*, Lois Schwartz* and Susan E. Quaggin*{dagger}

*The Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto, Ontario, Canada; and {dagger}Division of Nephrology, St. Michael’s Hospital, Toronto, Ontario, Canada.

Correspondence to: Susan E. Quaggin, The Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5. Phone: 416-586-4800; Fax: 416-586-8588; Email: quaggin{at}mshri.on.ca

Abstract

ABSTRACT. Podocytes (glomerular visceral epithelial cells) are highly specialized cells that are found in the renal glomerulus and make up a major portion of the filtration barrier between the blood and urinary spaces. Recently, the identification of a number of genes responsible for both autosomal dominant and recessive forms of human nephrotic syndrome has provided insight into a number of molecules responsible for unique features of the podocyte such as the slit diaphragms. Despite these major advances in our understanding of podocyte biology, the function of many genes expressed in the podocyte remains unknown. Targeted gene disruption using homologous recombination in murine embryonic stem cells (ES cells) is a powerful tool to determine the biologic function of genes in vivo. However, resulting embryonic lethal or pleiotropic phenotypes often preclude the analysis of genes in specific renal cell types. To overcome this problem, a glomerular-specific Cre-recombinase transgenic murine line under the control of the Nphs1 (nephrin) promoter (Neph-Cre) was generated. This article reports successful Cre-mediated excision of a ‘floxed’ transgene specifically in podocytes in vivo. This murine founder line represents a powerful new tool for the manipulation of the expression of genes in podocytes and will provide valuable insight into podocyte biology in the whole animal.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
P. Duann and E. A. Lianos
GEC-targeted HO-1 expression reduces proteinuria in glomerular immune injury
Am J Physiol Renal Physiol, September 1, 2009; 297(3): F629 - F638.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
T. B. Huber, B. Hartleben, K. Winkelmann, L. Schneider, J. U. Becker, M. Leitges, G. Walz, H. Haller, and M. Schiffer
Loss of Podocyte aPKC{lambda}/{iota} Causes Polarity Defects and Nephrotic Syndrome
J. Am. Soc. Nephrol., April 1, 2009; 20(4): 798 - 806.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Zhang, R. Ge, C. Matte-Martone, J. Goodwin, W. D. Shlomchik, M. J. Mamula, A. Kooshkabadi, M. P. Hardy, and D. Geller
Characterization of a Novel Gain of Function Glucocorticoid Receptor Knock-in Mouse
J. Biol. Chem., March 6, 2009; 284(10): 6249 - 6259.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
A. M. Waters, M. Y.J. Wu, T. Onay, J. Scutaru, J. Liu, C. G. Lobe, S. E. Quaggin, and T. D. Piscione
Ectopic Notch Activation in Developing Podocytes Causes Glomerulosclerosis
J. Am. Soc. Nephrol., June 1, 2008; 19(6): 1139 - 1157.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. L. Miller, P. Zhang, T. Chen, A. Rohrwasser, and R. D. Nelson
Automated method for the isolation of collecting ducts
Am J Physiol Renal Physiol, July 1, 2006; 291(1): F236 - F245.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. H. Miner, G. Go, J. Cunningham, B. L. Patton, and G. Jarad
Transgenic isolation of skeletal muscle and kidney defects in laminin {beta}2 mutant mice: implications for Pierson syndrome
Development, March 1, 2006; 133(5): 967 - 975.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. Juhila, R. Roozendaal, M. Lassila, S. J. Verbeek, and H. Holthofer
Podocyte Cell-Specific Expression of Doxycycline Inducible Cre Recombinase in Mice
J. Am. Soc. Nephrol., March 1, 2006; 17(3): 648 - 654.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
L. Wang, T. A. Fields, K. Pazmino, Q. Dai, J. L. Burchette, D. N. Howell, T. M. Coffman, and R. F. Spurney
Activation of G{alpha}q-Coupled Signaling Pathways in Glomerular Podocytes Promotes Renal Injury
J. Am. Soc. Nephrol., December 1, 2005; 16(12): 3611 - 3622.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. Belteki, J. Haigh, N. Kabacs, K. Haigh, K. Sison, F. Costantini, J. Whitsett, S. E. Quaggin, and A. Nagy
Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction
Nucleic Acids Res., March 22, 2005; 33(5): e51 - e51.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
G. Guo, D. J. Morrison, J. D. Licht, and S. E. Quaggin
WT1 Activates a Glomerular-Specific Enhancer Identified from the Human Nephrin Gene
J. Am. Soc. Nephrol., November 1, 2004; 15(11): 2851 - 2856.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
A. Gawlik and S. E. Quaggin
Deciphering the Renal Code: Advances in Conditional Gene Targeting
Physiology, October 1, 2004; 19(5): 245 - 252.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
I. Rubera, C. Poujeol, G. Bertin, L. Hasseine, L. Counillon, P. Poujeol, and M. Tauc
Specific Cre/Lox Recombination in the Mouse Proximal Tubule
J. Am. Soc. Nephrol., August 1, 2004; 15(8): 2050 - 2056.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
P. Igarashi
Kidney-Specific Gene Targeting
J. Am. Soc. Nephrol., August 1, 2004; 15(8): 2237 - 2239.
[Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
T. Benzing
Signaling at the Slit Diaphragm
J. Am. Soc. Nephrol., June 1, 2004; 15(6): 1382 - 1391.
[Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S. Hoffmann, D. Podlich, B. Hahnel, W. Kriz, and N. Gretz
Angiotensin II Type 1 Receptor Overexpression in Podocytes Induces Glomerulosclerosis in Transgenic Rats
J. Am. Soc. Nephrol., June 1, 2004; 15(6): 1475 - 1487.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. A. Bianco, H. L. Keen, J. L. Lavoie, and C. D. Sigmund
Untraditional methods for targeting the kidney in transgenic mice
Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1027 - F1033.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. K. Stricklett, D. Taylor, R. D. Nelson, and D. E. Kohan
Thick ascending limb-specific expression of Cre recombinase
Am J Physiol Renal Physiol, July 1, 2003; 285(1): F33 - F39.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J.-L. Michaud, L. I. Lemieux, M. Dube, B. C. Vanderhyden, S. J. Robertson, and C. R.J. Kennedy
Focal and Segmental Glomerulosclerosis in Mice with Podocyte-Specific Expression of Mutant {alpha}-Actinin-4
J. Am. Soc. Nephrol., May 1, 2003; 14(5): 1200 - 1211.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
L. Bugeon, A. Danou, D. Carpentier, P. Langridge, N. Syed, and M. J. Dallman
Inducible Gene Silencing in Podocytes: A New Tool for Studying Glomerular Function
J. Am. Soc. Nephrol., March 1, 2003; 14(3): 786 - 791.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. A. Kreidberg
Podocyte Differentiation and Glomerulogenesis
J. Am. Soc. Nephrol., March 1, 2003; 14(3): 806 - 814.
[Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
O. Beltcheva, S. Kontusaari, S. Fetissov, H. Putaala, P. Kilpelainen, T. Hokfelt, and K. Tryggvason
Alternatively Used Promoters and Distinct Elements Direct Tissue-Specific Expression of Nephrin
J. Am. Soc. Nephrol., February 1, 2003; 14(2): 352 - 358.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
H. Pavenstadt, W. Kriz, and M. Kretzler
Cell Biology of the Glomerular Podocyte
Physiol Rev, January 1, 2003; 83(1): 253 - 307.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
T. A. Natoli, J. Liu, V. Eremina, K. Hodgens, C. Li, Y. Hamano, P. Mundel, R. Kalluri, J. H. Miner, S. E. Quaggin, et al.
A Mutant Form of the Wilms' Tumor Suppressor Gene WT1 Observed in Denys-Drash Syndrome Interferes with Glomerular Capillary Development
J. Am. Soc. Nephrol., August 1, 2002; 13(8): 2058 - 2067.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
X. Shao, S. Somlo, and P. Igarashi
Epithelial-Specific Cre/lox Recombination in the Developing Kidney and Genitourinary Tract
J. Am. Soc. Nephrol., July 1, 2002; 13(7): 1837 - 1846.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S. E. Quaggin
A "Molecular Toolbox" for the Nephrologist
J. Am. Soc. Nephrol., June 1, 2002; 13(6): 1682 - 1685.
[Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2009 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673