Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peti-Peterdi, J.
Right arrow Articles by Bell, P. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peti-Peterdi, J.
Right arrow Articles by Bell, P. D.
J Am Soc Nephrol 13:1131-1135, 2002
© 2002 American Society of Nephrology

Angiotensin II Directly Stimulates ENaC Activity in the Cortical Collecting Duct via AT1 Receptors

János Peti-Peterdi, David G. Warnock and P. Darwin Bell

Nephrology Research and Training Center, Division of Nephrology, Departments of Medicine and Physiology, University of Alabama at Birmingham, Birmingham, Alabama.

Correspondence to: Dr. Janos Peti-Peterdi, University of Alabama at Birmingham, 865 Sparks Center, 1530 3rd Avenue South, Birmingham, AL 35294. Phone: 205-934-5783; Fax: 205-934-1147; E-mail: petjan{at}uab.edu

ABSTRACT. Angiotensin II (AngII) helps to regulate overall renal tubular reabsorption of salt and water, yet its effects in the distal nephron have not been well studied. The purpose of these studies was to determine whether AngII stimulates luminal Na+ transport in the cortical collecting duct (CCD). Intracellular Na+ concentration ([Na+]i), as a reflection of Na+ transport across the apical membrane, was measured with fluorescence microscopy using sodium-binding benzofuran isophthalate (SBFI) in isolated, perfused CCD segments dissected from rabbit kidneys. Control [Na+]i, during perfusion with 25 mM NaCl and a Na+-free solution in the bath containing the Na+-ionophore monensin (10 µM, to eliminate basolateral membrane Na+ transport) averaged 19.3 ± 5.2 mM (n = 16). Increasing luminal [NaCl] to 150 mM elevated [Na+]i by 9.87 ± 1.5 mM (n = 7; P < 0.05). AngII (10-9 M) added to the lumen significantly elevated baseline [Na+]i by 6.3 ± 1.0 mM and increased the magnitude ({Delta} = 25.2 ± 3.7 mM) and initial rate ({approx}5 fold) of change in [Na+]i to increased luminal [NaCl]. AngII when added to the bath had similar stimulatory effects; however, AngII was much more effective from the lumen. Thus, AngII significantly increased the apical entry of Na+ in the CCD. To determine if this apical entry step occurred via the epithelial Na+ channel (ENaC), studies were performed using the specific ENaC blocker, benzamil hydrochloride (10-6 M). When added to the perfusate, benzamil almost completely inhibited the elevations in [Na+]i to increased luminal [NaCl] in both the presence and absence of AngII. These results suggest that AngII directly stimulates Na+ channel activity in the CCD. AT1 receptor blockade with candesartan or losartan (10-6 M) prevented the stimulatory effects of AngII. Regulation of ENaC activity by AngII may play an important role in distal Na+ reabsorption in health and disease.




This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
J. Peti-Peterdi, J. J. Kang, and I. Toma
Activation of the renal renin-angiotensin system in diabetes--new concepts
Nephrol. Dial. Transplant., October 1, 2008; 23(10): 3047 - 3049.
[Full Text] [PDF]

Home page
 HypertensionHome page
D. Zhao and L. G. Navar
Acute Angiotensin II Infusions Elicit Pressure Natriuresis in Mice and Reduce Distal Fractional Sodium Reabsorption
Hypertension, July 1, 2008; 52(1): 137 - 142.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
J. J. Kang, I. Toma, A. Sipos, E. J. Meer, S. L. Vargas, and J. Peti-Peterdi
The Collecting Duct Is the Major Source of Prorenin in Diabetes
Hypertension, June 1, 2008; 51(6): 1597 - 1604.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. C. Prieto-Carrasquero, F. T. Botros, J. Pagan, H. Kobori, D. M. Seth, D. E. Casarini, and L. G. Navar
Collecting Duct Renin Is Upregulated in Both Kidneys of 2-Kidney, 1-Clip Goldblatt Hypertensive Rats
Hypertension, June 1, 2008; 51(6): 1590 - 1596.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
H. A. Drummond, S. C. Grifoni, and N. L. Jernigan
A New Trick for an Old Dogma: ENaC Proteins as Mechanotransducers in Vascular Smooth Muscle
Physiology, February 1, 2008; 23(1): 23 - 31.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
V. Pech, W. Zheng, T. D. Pham, J. W. Verlander, and S. M. Wall
Angiotensin II Activates H+-ATPase in Type A Intercalated Cells
J. Am. Soc. Nephrol., January 1, 2008; 19(1): 84 - 91.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
R. A. Fenton and M. A. Knepper
Mouse Models and the Urinary Concentrating Mechanism in the New Millennium
Physiol Rev, October 1, 2007; 87(4): 1083 - 1112.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. H. Kim, V. Pech, K. B. Spencer, W. H. Beierwaltes, L. A. Everett, E. D. Green, W. Shin, J. W. Verlander, R. L. Sutliff, and S. M. Wall
Reduced ENaC protein abundance contributes to the lower blood pressure observed in pendrin-null mice
Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1314 - F1324.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
H. Kobori, M. Nangaku, L. G. Navar, and A. Nishiyama
The Intrarenal Renin-Angiotensin System: From Physiology to the Pathobiology of Hypertension and Kidney Disease
Pharmacol. Rev., September 1, 2007; 59(3): 251 - 287.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. W. Verlander
The thiazide-sensitive NaCl cotransporter: a new target for acute regulation of salt and water transport by angiotensin II
Am J Physiol Renal Physiol, September 1, 2007; 293(3): F660 - F661.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
V. Pech, Y. H. Kim, A. M. Weinstein, L. A. Everett, T. D. Pham, and S. M. Wall
Angiotensin II increases chloride absorption in the cortical collecting duct in mice through a pendrin-dependent mechanism
Am J Physiol Renal Physiol, March 1, 2007; 292(3): F914 - F920.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Eskild-Jensen, K. Thomsen, C. Rungo, L. S. Ferreira, L. F. Paulsen, Y. F. Rawashdeh, J. R. Nyengaard, S. Nielsen, J. C. Djurhuus, and J. Frokiaer
Glomerular and tubular function during AT1 receptor blockade in pigs with neonatal induced partial ureteropelvic obstruction
Am J Physiol Renal Physiol, March 1, 2007; 292(3): F921 - F929.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
J.-M. Lalouel and A. Rohrwasser
Genetic Susceptibility to Essential Hypertension: Insight From Angiotensinogen
Hypertension, March 1, 2007; 49(3): 597 - 603.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S.D. Crowley, S.B. Gurley, M.J. Herrera, P. Ruiz, R. Griffiths, A.P. Kumar, H.-S. Kim, O. Smithies, T.H. Le, T.M. Coffman, et al.
Role of Renal Angiotensin II Type 1 Receptors in the Genesis of Hypertension: Guyton Revisited: Angiotensin II Causes Hypertension and Cardiac Hypertrophy through Its Receptors in the Kidney. Proc Natl Acad Sci U S A 103: 17985-17990, 2006
J. Am. Soc. Nephrol., February 1, 2007; 18(2): 356 - 363.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y.-J. Lee, I.-K. Song, K.-J. Jang, J. Nielsen, J. Frokiaer, S. Nielsen, and T.-H. Kwon
Increased AQP2 targeting in primary cultured IMCD cells in response to angiotensin II through AT1 receptor
Am J Physiol Renal Physiol, January 1, 2007; 292(1): F340 - F350.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. D. Crowley, S. B. Gurley, M. J. Herrera, P. Ruiz, R. Griffiths, A. P. Kumar, H.-S. Kim, O. Smithies, T. H. Le, and T. M. Coffman
Angiotensin II causes hypertension and cardiac hypertrophy through its receptors in the kidney
PNAS, November 21, 2006; 103(47): 17985 - 17990.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
D.-F. Guo, I. Chenier, J. L. Lavoie, J. S.D. Chan, P. Hamet, J. Tremblay, X. M. Chen, D. H. Wang, and T. Inagami
Development of Hypertension and Kidney Hypertrophy in Transgenic Mice Overexpressing ARAP1 Gene in the Kidney
Hypertension, September 1, 2006; 48(3): 453 - 459.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
H.-P. Ma and D. C. Eaton
Acute Regulation of Epithelial Sodium Channel by Anionic Phospholipids
J. Am. Soc. Nephrol., November 1, 2005; 16(11): 3182 - 3187.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. C. Prieto-Carrasquero, H. Kobori, Y. Ozawa, A. Gutierrez, D. Seth, and L. G. Navar
AT1 receptor-mediated enhancement of collecting duct renin in angiotensin II-dependent hypertensive rats
Am J Physiol Renal Physiol, September 1, 2005; 289(3): F632 - F637.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. Loghman-Adham, C. E. Soto, T. Inagami, and C. Sotelo-Avila
Expression of Components of the Renin-angiotensin System in Autosomal Recessive Polycystic Kidney Disease
J. Histochem. Cytochem., August 1, 2005; 53(8): 979 - 988.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
T.-H. Kwon, J. Nielsen, M. A. Knepper, J. Frokiaer, and S. Nielsen
Angiotensin II AT1 receptor blockade decreases vasopressin-induced water reabsorption and AQP2 levels in NaCl-restricted rats
Am J Physiol Renal Physiol, April 1, 2005; 288(4): F673 - F684.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
P. Valles, J. Wysocki, M. R. Salabat, I. Cokic, M. Ye, M. S. LaPointe, and D. Batlle
Angiotensin II Increases H+-ATPase B1 Subunit Expression in Medullary Collecting Ducts
Hypertension, April 1, 2005; 45(4): 818 - 823.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
H. Narumiya, S. Sasaki, N. Kuwahara, H. Irie, T. Kusaba, H. Kameyama, K. Tamagaki, T. Hatta, K. Takeda, and H. Matsubara
HMG-CoA reductase inhibitors up-regulate anti-aging klotho mRNA via RhoA inactivation in IMCD3 cells
Cardiovasc Res, November 1, 2004; 64(2): 331 - 336.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. Meneton, J. Loffing, and D. G. Warnock
Sodium and potassium handling by the aldosterone-sensitive distal nephron: the pivotal role of the distal and connecting tubule
Am J Physiol Renal Physiol, October 1, 2004; 287(4): F593 - F601.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
M. C. Prieto-Carrasquero, L. M. Harrison-Bernard, H. Kobori, Y. Ozawa, K. S. Hering-Smith, L. L. Hamm, and L. G. Navar
Enhancement of Collecting Duct Renin in Angiotensin II-Dependent Hypertensive Rats
Hypertension, August 1, 2004; 44(2): 223 - 229.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. W. Kim, W. Wang, J. Nielsen, J. Praetorius, T.-H. Kwon, M. A. Knepper, J. Frokiaer, and S. Nielsen
Increased expression and apical targeting of renal ENaC subunits in puromycin aminonucleoside-induced nephrotic syndrome in rats
Am J Physiol Renal Physiol, May 1, 2004; 286(5): F922 - F935.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. L. Lavoie, K. D. Lake-Bruse, and C. D. Sigmund
Increased blood pressure in transgenic mice expressing both human renin and angiotensinogen in the renal proximal tubule
Am J Physiol Renal Physiol, May 1, 2004; 286(5): F965 - F971.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
H. Kobori, M. C. Prieto-Carrasquero, Y. Ozawa, and L. G. Navar
AT1 Receptor Mediated Augmentation of Intrarenal Angiotensinogen in Angiotensin II-Dependent Hypertension
Hypertension, May 1, 2004; 43(5): 1126 - 1132.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Nielsen, T.-H. Kwon, J. Praetorius, Y.-H. Kim, J. Frokiaer, M. A. Knepper, and S. Nielsen
Segment-specific ENaC downregulation in kidney of rats with lithium-induced NDI
Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1198 - F1209.
[Abstract] [Full Text]

Home page
 HypertensionHome page
P. Komlosi, A. L. Fuson, A. Fintha, J. Peti-Peterdi, L. Rosivall, D. G. Warnock, and P. D. Bell
Angiotensin I Conversion to Angiotensin II Stimulates Cortical Collecting Duct Sodium Transport
Hypertension, August 1, 2003; 42(2): 195 - 199.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. Yuan, M. Liang, Z. Yang, E. Rute, N. Taylor, M. Olivier, and A. W. Cowley Jr.
Gene expression reveals vulnerability to oxidative stress and interstitial fibrosis of renal outer medulla to nonhypertensive elevations of ANG II
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2003; 284(5): R1219 - R1230.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
K. T. Beutler, S. Masilamani, S. Turban, J. Nielsen, H. L. Brooks, S. Ageloff, R. A. Fenton, R. K. Packer, and M. A. Knepper
Long-Term Regulation of ENaC Expression in Kidney by Angiotensin II
Hypertension, May 1, 2003; 41(5): 1143 - 1150.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Loffing and B. Kaissling
Sodium and calcium transport pathways along the mammalian distal nephron: from rabbit to human
Am J Physiol Renal Physiol, April 1, 2003; 284(4): F628 - F643.
[Abstract] [Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673