2007 JASN IMPACT FACTOR 7.111	HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP
advanced	CURRENT ISSUE	ARCHIVES	JASN Express	ONLINE SUBMISSION

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Asselman, M. Articles by Verkoelen, C. F. Search for Related Content PubMed PubMed Citation Articles by Asselman, M. Articles by Verkoelen, C. F.

Calcium Oxalate Crystal Adherence to Hyaluronan-, Osteopontin-, and CD44-Expressing Injured/Regenerating Tubular Epithelial Cells in Rat Kidneys

Marino Asselman^*, Anja Verhulst, Marc E. de Broe and Carl F. Verkoelen^*

*Department of Urology, Erasmus Medical Center Rotterdam, Rotterdam, the Netherlands; and Department of Nephrology-Hypertension, University of Antwerp, Antwerp, Belgium

Correspondence to Dr. Marc E. de Broe, University of Antwerp, Department of Nephrology-Hypertension, p/a University Hospital Antwerp, Wilrijkstraat 10, B-2650 Edegem/Antwerpen, Belgium; Phone: +32-3-821-3421; Fax: +32-3-829-0100; E-mail: debroe{at}uia.ua.ac.be

ABSTRACT. Retention of crystals in the kidney is an essential early step in renal stone formation. Studies with renal tubular cells in culture indicate that hyaluronan (HA) and osteopontin (OPN) and their mutual cell surface receptor CD44 play an important role in calcium oxalate (CaOx) crystal binding during wound healing. This concept was investigated in vivo by treating rats for 1, 4, and 8 d with ethylene glycol (0.5 and 0.75%) in their drinking water to induce renal tubular cell damage and CaOx crystalluria. Tubular injury was morphologically scored on periodic acid-Schiff–stained renal tissue sections and tissue repair assessed by immunohistochemical staining for proliferating cell nuclear antigen. CaOx crystals were visualized in periodic acid-Schiff–stained sections by polarized light microscopy, and renal calcium deposits were quantified with von Kossa staining. HA was visualized with HA-binding protein and OPN and CD44 immunohistochemically with specific antibodies and quantified with an image analyzer system. Already after 1 d of treatment, both concentrations of ethylene glycol induced hyperoxaluria and CaOx crystalluria. At this point, there was neither tubular injury nor crystal retention in the kidney, and expression of HA, OPN, and CD44 was comparable to untreated controls. After 4 and 8 d of ethylene glycol, however, intratubular crystals were found adhered to injured/regenerating (proliferating cell nuclear antigen positive) tubular epithelial cells, expressing HA, OPN, and CD44 at their luminal membrane. In conclusion, the expression of HA, OPN, and CD44 by injured/regenerating tubular cells seems to play a role in retention of crystals in the rat kidney.

This article has been cited by other articles:

				C. F. Verkoelen Crystal Retention in Renal Stone Disease: A Crucial Role for the Glycosaminoglycan Hyaluronan? J. Am. Soc. Nephrol., June 1, 2006; 17(6): 1673 - 1687. [Abstract] [Full Text] [PDF]

				M. L. Green, M. Hatch, and R. W. Freel Ethylene glycol induces hyperoxaluria without metabolic acidosis in rats Am J Physiol Renal Physiol, September 1, 2005; 289(3): F536 - F543. [Abstract] [Full Text] [PDF]

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673