2007 JASN IMPACT FACTOR 7.111	HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP
advanced	CURRENT ISSUE	ARCHIVES	JASN Express	ONLINE SUBMISSION

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Quentin, F. Articles by Chambrey, R. Search for Related Content PubMed PubMed Citation Articles by Quentin, F. Articles by Chambrey, R.

RhBG and RhCG, the Putative Ammonia Transporters, Are Expressed in the Same Cells in the Distal Nephron

Fabienne Quentin^*, Dominique Eladari^*,, Lydie Cheval, Claude Lopez, Dominique Goossens, Yves Colin, Jean-Pierre Cartron, Michel Paillard^*, and Régine Chambrey^*

*Institut National de la Santé et de la Recherche Médicale Unité 356, Institut Fédératif de Recherche 58, Université Pierre et Marie Curie, Paris, France; Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, Paris, France; Centre National de la Recherche Scientifique FRE 2468, Paris, France; and Institut National de la Santé et de la Recherche Médicale Unité 76, Institut National de la Transfusion Sanguine, Paris, France.

Correspondence to Dr. Régine Chambrey, Unité INSERM 356, Institut de Recherche des Cordeliers, 15 rue de l’Ecole de Médecine, 75270 Paris Cedex 06, France. Phone: 33-1-44-41-37-10; Fax: 33-1-44-41-37-17

ABSTRACT. Two nonerythroid homologs of the blood group Rh proteins, RhCG and RhBG, which share homologies with specific ammonia transporters in primitive organisms and plants, could represent members of a new family of proteins involved in ammonia transport in the mammalian kidney. Consistent with this hypothesis, the expression of RhCG was recently reported at the apical pole of all connecting tubule (CNT) cells as well as in intercalated cells of collecting duct (CD). To assess the localization along the nephron of RhBG, polyclonal antibodies against the Rh type B glycoprotein were generated. In immunoblot experiments, a specific polypeptide of Mr approximately 50 kD was detected in rat kidney cortex and in outer and inner medulla membrane fractions. Immunocytochemical studies revealed RhBG expression in distal nephron segments within the cortical labyrinth, medullary rays, and outer and inner medulla. RhBG expression was restricted to the basolateral membrane of epithelial cells. The same localization was observed in rat and mouse kidney. RT-PCR analysis on microdissected rat nephron segments confirmed that RhBG mRNAs were chiefly expressed in CNT and cortical and outer medullary CD. Double immunostaining with RhCG demonstrated that RhBG and RhCG were coexpressed in the same cells, but with a basolateral and apical localization, respectively. In conclusion, RhBG and RhCG are present in a major site of ammonia secretion in the kidney, i.e., the CNT and CD, in agreement with their putative role in ammonium transport. E-mail: chambrey@ccr.jussieu.fr

This article has been cited by other articles:

				F. Sohet, Y. Colin, S. Genetet, P. Ripoche, S. Metral, C. Le Van Kim, and C. Lopez Phosphorylation and Ankyrin-G Binding of the C-terminal Domain Regulate Targeting and Function of the Ammonium Transporter RhBG J. Biol. Chem., September 26, 2008; 283(39): 26557 - 26567. [Abstract] [Full Text] [PDF]

				M. Boeckstaens, B. Andre, and A. M. Marini Distinct Transport Mechanisms in Yeast Ammonium Transport/Sensor Proteins of the Mep/Amt/Rh Family and Impact on Filamentation J. Biol. Chem., August 1, 2008; 283(31): 21362 - 21370. [Abstract] [Full Text] [PDF]

				T. Nakada, K. Hoshijima, M. Esaki, S. Nagayoshi, K. Kawakami, and S. Hirose Localization of ammonia transporter Rhcg1 in mitochondrion-rich cells of yolk sac, gill, and kidney of zebrafish and its ionic strength-dependent expression Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1743 - R1753. [Abstract] [Full Text] [PDF]

				H.-Y. Kim, C. Baylis, J. W. Verlander, K.-H. Han, S. Reungjui, M. E. Handlogten, and I. D. Weiner Effect of reduced renal mass on renal ammonia transporter family, Rh C glycoprotein and Rh B glycoprotein, expression Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1238 - F1247. [Abstract] [Full Text] [PDF]

				K.-H. Han, H.-Y. Kim, B. P. Croker, S. Reungjui, S.-Y. Lee, J. Kim, M. E. Handlogten, C. A. Adin, and I. D. Weiner Effects of ischemia-reperfusion injury on renal ammonia metabolism and the collecting duct Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1342 - F1354. [Abstract] [Full Text] [PDF]

				C. Y. C. Hung, K. N. T. Tsui, J. M. Wilson, C. M. Nawata, C. M. Wood, and P. A. Wright Rhesus glycoprotein gene expression in the mangrove killifish Kryptolebias marmoratus exposed to elevated environmental ammonia levels and air J. Exp. Biol., July 15, 2007; 210(14): 2419 - 2429. [Abstract] [Full Text] [PDF]

				T. Nakada, C. M. Westhoff, A. Kato, and S. Hirose Ammonia secretion from fish gill depends on a set of Rh glycoproteins FASEB J, April 1, 2007; 21(4): 1067 - 1074. [Abstract] [Full Text] [PDF]

				S. Khademi and R. M. Stroud The Amt/MEP/Rh Family: Structure of AmtB and the Mechanism of Ammonia Gas Conduction. Physiology, December 1, 2006; 21(6): 419 - 429. [Abstract] [Full Text] [PDF]

				L. Cheval, L. Morla, J.-M. Elalouf, and A. Doucet Kidney collecting duct acid-base "regulon" Physiol Genomics, November 21, 2006; 27(3): 271 - 281. [Abstract] [Full Text] [PDF]

				K.-H. Han, B. P. Croker, W. L. Clapp, D. Werner, M. Sahni, J. Kim, H.-Y. Kim, M. E. Handlogten, and I. D. Weiner Expression of the Ammonia Transporter, Rh C Glycoprotein, in Normal and Neoplastic Human Kidney J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2670 - 2679. [Abstract] [Full Text] [PDF]

				R. M. Seshadri, J. D. Klein, T. Smith, J. M. Sands, M. E. Handlogten, J. W. Verlander, and I. D. Weiner Changes in subcellular distribution of the ammonia transporter, Rhcg, in response to chronic metabolic acidosis Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1443 - F1452. [Abstract] [Full Text] [PDF]

				Q. Ji, S. Hashmi, Z. Liu, J. Zhang, Y. Chen, and C.-H. Huang CeRh1 (rhr-1) is a dominant Rhesus gene essential for embryonic development and hypodermal function in Caenorhabditis elegans PNAS, April 11, 2006; 103(15): 5881 - 5886. [Abstract] [Full Text] [PDF]

				D.-O. D. Mak, B. Dang, I. D. Weiner, J. K. Foskett, and C. M. Westhoff Characterization of ammonia transport by the kidney Rh glycoproteins RhBG and RhCG Am J Physiol Renal Physiol, February 1, 2006; 290(2): F297 - F305. [Abstract] [Full Text] [PDF]

				R. M. Seshadri, J. D. Klein, S. Kozlowski, J. M. Sands, Y.-H. Kim, K.-H. Han, M. E. Handlogten, J. W. Verlander, and I. D. Weiner Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis Am J Physiol Renal Physiol, February 1, 2006; 290(2): F397 - F408. [Abstract] [Full Text] [PDF]

				M. Mayer, G. Schaaf, I. Mouro, C. Lopez, Y. Colin, P. Neumann, J.-P. Cartron, and U. Ludewig Different Transport Mechanisms in Plant and Human AMT/Rh-type Ammonium Transporters J. Gen. Physiol., January 30, 2006; 127(2): 133 - 144. [Abstract] [Full Text] [PDF]

				R. Chambrey, D. Goossens, S. Bourgeois, N. Picard, M. Bloch-Faure, F. Leviel, V. Geoffroy, M. Cambillau, Y. Colin, M. Paillard, et al. Genetic ablation of Rhbg in the mouse does not impair renal ammonium excretion Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1281 - F1290. [Abstract] [Full Text] [PDF]

				C.-H. Huang and J. Peng Evolutionary conservation and diversification of Rh family genes and proteins PNAS, October 25, 2005; 102(43): 15512 - 15517. [Abstract] [Full Text] [PDF]

				M. E. Handlogten, S.-P. Hong, C. M. Westhoff, and I. D. Weiner Apical ammonia transport by the mouse inner medullary collecting duct cell (mIMCD-3) Am J Physiol Renal Physiol, August 1, 2005; 289(2): F347 - F358. [Abstract] [Full Text] [PDF]

				M. E. Handlogten, S.-P. Hong, L. Zhang, A. W. Vander, M. L. Steinbaum, M. Campbell-Thompson, and I. D. Weiner Expression of the ammonia transporter proteins Rh B glycoprotein and Rh C glycoprotein in the intestinal tract Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G1036 - G1047. [Abstract] [Full Text] [PDF]

				C. Lopez, S. Metral, D. Eladari, S. Drevensek, P. Gane, R. Chambrey, V. Bennett, J.-P. Cartron, C. Le Van Kim, and Y. Colin The Ammonium Transporter RhBG: REQUIREMENT OF A TYROSINE-BASED SIGNAL AND ANKYRIN-G FOR BASOLATERAL TARGETING AND MEMBRANE ANCHORAGE IN POLARIZED KIDNEY EPITHELIAL CELLS J. Biol. Chem., March 4, 2005; 280(9): 8221 - 8228. [Abstract] [Full Text] [PDF]

				C. A. Wagner, K. E. Finberg, S. Breton, V. Marshansky, D. Brown, and J. P. Geibel Renal Vacuolar H+-ATPase Physiol Rev, October 1, 2004; 84(4): 1263 - 1314. [Abstract] [Full Text] [PDF]

				M. E. Handlogten, S.-P. Hong, C. M. Westhoff, and I. D. Weiner Basolateral ammonium transport by the mouse inner medullary collecting duct cell (mIMCD-3) Am J Physiol Renal Physiol, October 1, 2004; 287(4): F628 - F638. [Abstract] [Full Text] [PDF]

				L. Cheval, J. P. Duong Van Huyen, P. Bruneval, J.-M. Verbavatz, J.-M. Elalouf, and A. Doucet Plasticity of mouse renal collecting duct in response to potassium depletion Physiol Genomics, September 16, 2004; 19(1): 61 - 73. [Abstract] [Full Text] [PDF]

				U. Ludewig Electroneutral ammonium transport by basolateral rhesus B glycoprotein J. Physiol., September 15, 2004; 559(3): 751 - 759. [Abstract] [Full Text] [PDF]

				G. Garibotto, A. Sofia, C. Robaudo, S. Saffioti, M. R. Sala, D. Verzola, M. Vettore, R. Russo, V. Procopio, G. Deferrari, et al. Kidney Protein Dynamics and Ammoniagenesis in Humans with Chronic Metabolic Acidosis J. Am. Soc. Nephrol., June 1, 2004; 15(6): 1606 - 1615. [Abstract] [Full Text] [PDF]

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673