Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bakker, R. C.
Right arrow Articles by Paul, L. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bakker, R. C.
Right arrow Articles by Paul, L. C.
J Am Soc Nephrol 14:2142-2149, 2003
© 2003 American Society of Nephrology

Early Interstitial Accumulation of Collagen Type I Discriminates Chronic Rejection from Chronic Cyclosporine Nephrotoxicity

Rene C. Bakker*, Klaas Koop{dagger}, Yvo W. Sijpkens*, Michael Eikmans{dagger}, Ingeborg M. Bajema{dagger}, Emile de Heer{dagger}, Jan A. Bruijn{dagger} and Leendert C. Paul*

Departments of *Nephrology and {dagger}Pathology, Leiden University Medical Center, Leiden, The Netherlands.

Correspondence to Dr. Rene C. Bakker, Department of Nephrology, Leiden University Medical Center, Building 1, C3-P, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone: +31-715262148; Fax: +31-715248118;

ABSTRACT. Little is known regarding the composition of the interstitial extracellular matrix of kidney allografts with deteriorating function. Collagen I, III, and IV, the collagen IV {alpha}3 chain, and the laminin {beta}2 chain were investigated in biopsies of allografted kidneys with chronic cyclosporine A nephrotoxicity (CsAT) (n = 17), chronic rejection (CR) (n = 12), or chronic allograft nephropathy (CAN) (n = 19). {alpha}-Smooth muscle actin expression was also examined. Normal native kidneys were used as control samples (n = 11). Biopsy samples were studied with routine light microscopy and immunostaining. The mean interstitial fibrosis scores were significantly higher for the CR and CAN groups, compared with the chronic CsAT group. The cortical tubulointerstitial areas of the CR and CAN groups, but not the chronic CsAT group, contained more collagen I than did normal control samples. Differences were noted even in biopsies with mild fibrosis. Accumulation of collagen III, IV, and IV {alpha}3 was increased in all patient groups. Collagen III accumulation was greater in the CR and CAN groups than in the chronic CsAT group. Receiver-operating characteristic curve analysis demonstrated that collagen I staining had the best discriminatory value in differentiating CR from chronic CsAT, with a sensitivity of 63% and a specificity of 94% at a cutoff value of 19%. Laminin {beta}2 staining did not differentiate CR from CsAT. Increased {alpha}-smooth muscle actin staining did not differ among the three groups. It was concluded that, during chronic CsAT, collagen III and IV were preferentially accumulated in the tubulointerstitium. Early increases in the deposition of collagen I, with collagen III and IV, were more specific for CR. CR seems to elicit a more pronounced fibrotic response than does chronic CsAT. E-mail: RNR@euronet.nl




This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
E. M. Scholten, A. T. Rowshani, S. Cremers, F. J. Bemelman, M. Eikmans, E. van Kan, M. J. Mallat, S. Florquin, J. Surachno, I. J. ten Berge, et al.
Untreated Rejection in 6-Month Protocol Biopsies Is Not Associated with Fibrosis in Serial Biopsies or with Loss of Graft Function
J. Am. Soc. Nephrol., September 1, 2006; 17(9): 2622 - 2632.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. C. Roos-van Groningen, E. M. Scholten, P. M. Lelieveld, A. T. Rowshani, H. J. Baelde, I. M. Bajema, S. Florquin, F. J. Bemelman, E. de Heer, J. W. de Fijter, et al.
Molecular Comparison of Calcineurin Inhibitor-Induced Fibrogenic Responses in Protocol Renal Transplant Biopsies
J. Am. Soc. Nephrol., March 1, 2006; 17(3): 881 - 888.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. Eikmans, M. C. Roos-van Groningen, Y. W.J. Sijpkens, J. Ehrchen, J. Roth, H. J. Baelde, I. M. Bajema, J. W. de Fijter, E. de Heer, and J. A. Bruijn
Expression of Surfactant Protein-C, S100A8, S100A9, and B Cell Markers in Renal Allografts: Investigation of the Prognostic Value
J. Am. Soc. Nephrol., December 1, 2005; 16(12): 3771 - 3786.
[Abstract] [Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673