2007 JASN IMPACT FACTOR 7.111	HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP
advanced	CURRENT ISSUE	ARCHIVES	JASN Express	ONLINE SUBMISSION

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gross, M.-L. Articles by Ritz, E. Search for Related Content PubMed PubMed Citation Articles by Gross, M.-L. Articles by Ritz, E.

Beneficial Effects of Estrogens on Indices of Renal Damage in Uninephrectomized SHRsp Rats

Marie-Luise Gross^*, Marcin Adamczak^,, Thomas Rabe, Nevin Ali Harbi, Jan Krtil, Andreas Koch^*, Peter Hamar^||, Kerstin Amann^¶ and Eberhard Ritz

Departments of *Pathology and Internal Medicine, University of Heidelberg, Heidelberg, Germany; Department of Nephrology, Endocrinology, and Metabolic Diseases, Medical University of Silesia, Katowice, Poland; Department of Gynecological Endocrinology and Reproductive Medicine, University Women’s Hospital, Heidelberg, Germany; ^||Institute of Pathophysiology, Semmelweis University, Budapest, Hungary; and ^¶Department of Pathology, University of Erlangen, Erlangen, Germany.

Correspondence to Dr. Marie-Luise Gross, Department of Pathology, University of Heidelberg, Im Neuenheimer Feld 220/221, 69120 Heidelberg, Germany. Phone: 0049-6221-562668; Fax: 0049-6221-455251; E-mail: Marie-Luise_Gross{at}med.uni-heidelberg.de

ABSTRACT. Renal diseases tend to be less severe among premenopausal female patients, compared with male patients. Experimental data on the effects of estrogens on renal damage are controversial, and potential underlying mechanisms have not been fully clarified. Three-month-old, female, uninephrectomized (UNX), sham-operated or ovariectomized (OVX) SHRsp rats were left untreated or received either 17-estradiol 3-benzoate (25 µg/d) or estriol (0.02 mg/d) daily. After 3 mo, indices of renal damage (glomerulosclerosis index and tubulointerstitial damage index) and glomerular geometric parameters were investigated. The expression of desmin, TGF-, endothelin-1, collagen IV, endothelial nitric oxide synthase, and estrogen receptors and in the glomeruli and tubulointerstitium was immunohistochemically evaluated. Estradiol and estriol did not significantly affect kidney weights or BP. Estradiol and estriol caused significant reductions in albuminuria (vehicle-treated UNX/OVX animals, 25.4 ± 8.52 mg/24 h; estradiol-treated UNX/OVX animals, 15.37 ± 6.12 mg/24 h; estriol-treated UNX/OVX animals, 6.54 ± 2.24 mg/24 h). The glomerulosclerosis index was significantly lower in estriol- and estradiol-treated animals (estradiol-treated UNX/OVX animals, 0.69 ± 0.16; estriol-treated UNX/OVX animals, 0.21 ± 0.12; P < 0.05), compared with vehicle-treated animals (1.46 ± 0.09); the tubulointerstitial damage index exhibited a similar pattern. The mean glomerular volume was significantly less in estrogen-treated animals. UNX/OVX animals demonstrated significantly greater expression of TGF- and endothelin-1 in immunohistochemical, in situ hybridization, and reverse transcription-PCR assays. This increase was abrogated by estriol but not estradiol. Similarly, significantly higher glomerular and tubulointerstitial expression of proliferating cell nuclear antigen and collagen IV was observed in UNX/OVX animals, and expression was decreased by estriol but not estradiol. It was concluded that, in the UNX model of spontaneous renal damage, glomerular lesions and glomerular hypertrophy were reduced by estriol but less consistently by estradiol. In parallel, loss of podocytes, evidence of podocyte injury (i.e., desmin expression), and expression of mediator systems of glomerular damage were decreased, pointing to a major renoprotective action of estriol.

This article has been cited by other articles:

				L. L. Yanes, J. C. Sartori-Valinotti, and J. F. Reckelhoff Sex Steroids and Renal Disease: Lessons From Animal Studies Hypertension, April 1, 2008; 51(4): 976 - 981. [Full Text] [PDF]

				S. Tiwari, S. Riazi, and C. A. Ecelbarger Insulin's impact on renal sodium transport and blood pressure in health, obesity, and diabetes Am J Physiol Renal Physiol, October 1, 2007; 293(4): F974 - F984. [Abstract] [Full Text] [PDF]

				J. C. Sullivan, L. Semprun-Prieto, E. I. Boesen, D. M. Pollock, and J. S. Pollock Sex and sex hormones influence the development of albuminuria and renal macrophage infiltration in spontaneously hypertensive rats Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1573 - R1579. [Abstract] [Full Text] [PDF]

				S. B. Ahmed, N. D.L. Fisher, and N. K. Hollenberg Gender and the Renal Nitric Oxide Synthase System in Healthy Humans Clin. J. Am. Soc. Nephrol., September 1, 2007; 2(5): 926 - 931. [Abstract] [Full Text] [PDF]

				G.Y. Oudit, A.M. Herzenberg, Z. Kassiri, D. Wong, H. Reich, R. Khokha, M.A. Crackower, P.H. Backx, J.M. Penninger, J.W. Scholey, et al. Angiotensin-Converting Enzyme-2 (ACE2)--A New Player in the Genesis of Glomerular Injury?: Loss of Angiotensin-Converting Enzyme-2 Leads to the Late Development of Angiotensin II-Dependent Glomerulosclerosis. Am J Pathol 168: 1808-1820, 2006 J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2637 - 2643. [Full Text] [PDF]

				M. Karl, M. Berho, J. Pignac-Kobinger, G. E. Striker, and S. J. Elliot Differential Effects of Continuous and Intermittent 17{beta}-Estradiol Replacement and Tamoxifen Therapy on the Prevention of Glomerulosclerosis: Modulation of the Mesangial Cell Phenotype in Vivo Am. J. Pathol., August 1, 2006; 169(2): 351 - 361. [Abstract] [Full Text] [PDF]

				G. Y. Oudit, A. M. Herzenberg, Z. Kassiri, D. Wong, H. Reich, R. Khokha, M. A. Crackower, P. H. Backx, J. M. Penninger, and J. W. Scholey Loss of Angiotensin-Converting Enzyme-2 Leads to the Late Development of Angiotensin II-Dependent Glomerulosclerosis Am. J. Pathol., June 1, 2006; 168(6): 1808 - 1820. [Abstract] [Full Text] [PDF]

				M. Nagase, S. Shibata, S. Yoshida, T. Nagase, T. Gotoda, and T. Fujita Podocyte Injury Underlies the Glomerulopathy of Dahl Salt-Hypertensive Rats and Is Reversed by Aldosterone Blocker Hypertension, June 1, 2006; 47(6): 1084 - 1093. [Abstract] [Full Text] [PDF]

				L. Wang, T. A. Fields, K. Pazmino, Q. Dai, J. L. Burchette, D. N. Howell, T. M. Coffman, and R. F. Spurney Activation of G{alpha}q-Coupled Signaling Pathways in Glomerular Podocytes Promotes Renal Injury J. Am. Soc. Nephrol., December 1, 2005; 16(12): 3611 - 3622. [Abstract] [Full Text] [PDF]

				C. Maric Sex Differences in Cardiovascular Disease and Hypertension: Involvement of the Renin-Angiotensin System Hypertension, September 1, 2005; 46(3): 475 - 476. [Full Text] [PDF]

				M. Chin, M. Isono, K. Isshiki, S.-i. Araki, T. Sugimoto, B. Guo, H. Sato, M. Haneda, A. Kashiwagi, and D. Koya Estrogen and Raloxifene, a Selective Estrogen Receptor Modulator, Ameliorate Renal Damage in db/db Mice Am. J. Pathol., June 1, 2005; 166(6): 1629 - 1636. [Abstract] [Full Text] [PDF]

				R. W. Mankhey, F. Bhatti, and C. Maric 17{beta}-Estradiol replacement improves renal function and pathology associated with diabetic nephropathy Am J Physiol Renal Physiol, February 1, 2005; 288(2): F399 - F405. [Abstract] [Full Text] [PDF]

				Y. Liu Hepatocyte growth factor in kidney fibrosis: therapeutic potential and mechanisms of action Am J Physiol Renal Physiol, July 1, 2004; 287(1): F7 - F16. [Abstract] [Full Text] [PDF]

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673