Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gertner, R. A.
Right arrow Articles by Sands, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gertner, R. A.
Right arrow Articles by Sands, J. M.
J Am Soc Nephrol 15:558-565, 2004
© 2004 American Society of Nephrology

BASIC SCIENCE

Aldosterone Decreases UT-A1 Urea Transporter Expression via the Mineralocorticoid Receptor

Randy A. Gertner*, Janet D. Klein*, James L. Bailey*, Dong-un Kim*, Xiao H. Luo*, Serena M. Bagnasco{dagger} and Jeff M. Sands*,{ddagger}

*Renal Division, Department of Medicine, {dagger}Department of Pathology, and {ddagger}Department of Physiology, Emory University School of Medicine, Atlanta, Georgia

Correspondence to Dr. Randy A. Gertner, Emory University School of Medicine, Renal Division, WMRB Room 338, 1639 Pierce Drive, NE, Atlanta, GA 30322. Phone: 404-727-2525; FAX: 404-727-3425; E-mail: rag145{at}hotmail.com

ABSTRACT. Adrenalectomy in rats is associated with urinary concentrating and diluting defects. This study tested the effect of adrenal steroids on the UT-A1 urea transporter because it is involved in the urine-concentrating mechanism. Rats were adrenalectomized and given normal saline for 14 d, after which they received (1) vehicle, (2) aldosterone, or (3) spironolactone plus aldosterone. Adrenalectomy alone significantly increased UT-A1 protein in the inner medullary tip after 7 d, whereas aldosterone repletion reversed the effect. Spironolactone blocked the aldosterone-induced decrease in UT-A1, indicating that aldosterone was working via the mineralocorticoid receptor. For verifying that glucocorticoids downregulate UT-A1 protein through a different receptor, three groups of adrenalectomized rats were prepared: (1) vehicle, (2) adrenalectomy plus dexamethasone, and (3) adrenalectomy plus dexamethasone and spironolactone. Dexamethasone significantly reversed UT-A1 protein abundance increase in the inner medullary tip of adrenalectomized rats. When spironolactone was given with dexamethasone, it did not affect the dexamethasone-induced decrease in UT-A1. There was no significant change in serum vasopressin level, aquaporin 2, or Na+-K+-2Cl- co-transporter NKCC2/BSC1 protein abundances or UT-A1 mRNA abundance in any of the groups. In conclusion, either mineralocorticoids or glucocorticoids can downregulate UT-A1 protein. The decrease in UT-A1 does not require both steroid hormones, and each works through a different receptor.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
J. D. Klein, B. P. Murrell, S. Tucker, Y.-H. Kim, and J. M. Sands
Urea transporter UT-A1 and aquaporin-2 proteins decrease in response to angiotensin II or norepinephrine-induced acute hypertension
Am J Physiol Renal Physiol, November 1, 2006; 291(5): F952 - F959.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. Y. Yang, W. Y. Tam, S. Tam, H. Guo, X. Wu, G. Li, J. F. L. Chau, J. D. Klein, S. K. Chung, J. M. Sands, et al.
Genetic restoration of aldose reductase to the collecting tubules restores maturation of the urine concentrating mechanism
Am J Physiol Renal Physiol, July 1, 2006; 291(1): F186 - F195.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
N. Konno, S. Hyodo, K. Matsuda, and M. Uchiyama
Effect of osmotic stress on expression of a putative facilitative urea transporter in the kidney and urinary bladder of the marine toad, Bufo marinus
J. Exp. Biol., April 1, 2006; 209(7): 1207 - 1216.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S.-W. Lim, K.-H. Han, J.-Y. Jung, W.-Y. Kim, C.-W. Yang, J. M. Sands, M. A. Knepper, K. M. Madsen, and J. Kim
Ultrastructural localization of UT-A and UT-B in rat kidneys with different hydration status
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2006; 290(2): R479 - R492.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Inoue, S. D. Kozlowski, J. D. Klein, J. L. Bailey, J. M. Sands, and S. M. Bagnasco
Regulated expression of renal and intestinal UT-B urea transporter in response to varying urea load
Am J Physiol Renal Physiol, August 1, 2005; 289(2): F451 - F458.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
R. A. Fenton, A. Flynn, A. Shodeinde, C. P. Smith, J. Schnermann, and M. A. Knepper
Renal Phenotype of UT-A Urea Transporter Knockout Mice
J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1583 - 1592.
[Abstract] [Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673