Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshizawa, N.
Right arrow Articles by Boyle, M. D.P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshizawa, N.
Right arrow Articles by Boyle, M. D.P.
J Am Soc Nephrol 15:1785-1793, 2004
© 2004 American Society of Nephrology

BASIC SCIENCE

Nephritis-Associated Plasmin Receptor and Acute Poststreptococcal Glomerulonephritis: Characterization of the Antigen and Associated Immune Response

Nobuyuki Yoshizawa*, Kazuo Yamakami*, Masayuki Fujino*, Takashi Oda*, Kikuko Tamura{dagger}, Koichi Matsumoto{ddagger}, Tetsuzo Sugisaki§ and Michael D.P. Boyle

Departments of *Public Health and {dagger}Pediatrics, National Defense Medical College, Saitama, {ddagger}Second Department of Internal Medicine, Nihon University School of Medicine, and §Department of Nephrology, Showa University School of Medicine, Tokyo, Japan; and Department of Biology, Juniata College, Huntington, Pennsylvania.

Correspondence to Dr. Nobuyuki Yoshizawa, Department of Public Health, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan. Phone: 81-4-2995-1575; Fax: 81-4-2996-5196; E-mail: yoshizawa-npr{at}umin.ac.jp

ABSTRACT. The role of nephritis-associated antigen as a virulence factor for acute poststreptococcal glomerulonephritis (APSGN) remains to be fully clarified. Nephritis-associated plasmin receptor (NAPlr) was previously isolated from group A streptococcus (GAS) and shown to bind plasmin(ogen). The nucleotide sequence of the naplr gene from GAS isolates obtained from patients with APSGN was determined. The sequence of the putative open reading frame (1011 bp) showed 99.8% identity among isolated strains. Homology screen revealed an exact match with streptococcal glyceraldehyde-3-phosphate dehydrogenase (GAPDH). NAPlr exhibited GAPDH activity in zymography, and it activated the complement pathway in vitro. In APSGN kidney biopsy specimens, NAPlr was observed mainly in the early stage of the disease (1 to 14 d after onset) but was not colocalized with either C3 or IgG as assessed by double immunofluorescence staining. Sera of patients with APSGN, patients with GAS infection without renal involvement, nonrenal pediatric patients, and healthy adults as controls were assayed for anti-NAPlr antibody titers. Anti-NAPlr antibodies were present most frequently in APSGN sera, and antibody titers were also significantly higher than in patients with GAS infection alone or in other control patients. Moreover, antibody titers remained elevated during the entire 10-yr follow-up period.




This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
B. Rodriguez-Iturbe and J. M. Musser
The Current State of Poststreptococcal Glomerulonephritis
J. Am. Soc. Nephrol., October 1, 2008; 19(10): 1855 - 1864.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
T. Oda, K. Tamura, N. Yoshizawa, T. Sugisaki, K. Matsumoto, M. Hattori, T. Sawai, T. Namikoshi, M. Yamada, Y. Kikuchi, et al.
Elevated urinary plasmin activity resistant to {alpha}2-antiplasmin in acute poststreptococcal glomerulonephritis
Nephrol. Dial. Transplant., July 1, 2008; 23(7): 2254 - 2259.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
T. Oda, N. Yoshizawa, K. Yamakami, A. Ishida, O. Hotta, S. Suzuki, and S. Miura
Significance of glomerular cell apoptosis in the resolution of acute post-streptococcal glomerulonephritis
Nephrol. Dial. Transplant., March 1, 2007; 22(3): 740 - 748.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
A. Servais, V. Fremeaux-Bacchi, M. Lequintrec, R. Salomon, J. Blouin, B. Knebelmann, J.-P. Grunfeld, P. Lesavre, L.-H. Noel, and F. Fakhouri
Primary glomerulonephritis with isolated C3 deposits: a new entity which shares common genetic risk factors with haemolytic uraemic syndrome
J. Med. Genet., March 1, 2007; 44(3): 193 - 199.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. R. Graham, K. Virtaneva, S. F. Porcella, D. J. Gardner, R. D. Long, D. M. Welty, W. T. Barry, C. A. Johnson, L. D. Parkins, F. A. Wright, et al.
Analysis of the Transcriptome of Group A Streptococcus in Mouse Soft Tissue Infection
Am. J. Pathol., September 1, 2006; 169(3): 927 - 942.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
T. Oda, K. Yamakami, F. Omasu, S. Suzuki, S. Miura, T. Sugisaki, and N. Yoshizawa
Glomerular Plasmin-Like Activity in Relation to Nephritis-Associated Plasmin Receptor in Acute Poststreptococcal Glomerulonephritis
J. Am. Soc. Nephrol., January 1, 2005; 16(1): 247 - 254.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
B. Rodriguez-Iturbe
Nephritis-Associated Streptococcal Antigens: Where Are We Now?
J. Am. Soc. Nephrol., July 1, 2004; 15(7): 1961 - 1962.
[Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673