2007 JASN IMPACT FACTOR 7.111	HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP
advanced	CURRENT ISSUE	ARCHIVES	JASN Express	ONLINE SUBMISSION

This Article Full Text Full Text (PDF) All Versions of this Article: ASN.2004080635v1 16/5/1471    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Agalou, S. Articles by Thornalley, P. J. Search for Related Content PubMed PubMed Citation Articles by Agalou, S. Articles by Thornalley, P. J.

Profound Mishandling of Protein Glycation Degradation Products in Uremia and Dialysis

Stamatina Agalou^*, Naila Ahmed^*, Roya Babaei-Jadidi^*, Anne Dawnay and Paul J. Thornalley^*

^* Department of Biological Sciences, University of Essex, Colchester, Essex; Renal Research Laboratory, St. Bartholomew’s and The Royal London School of Medicine and Dentistry, St. Bartholomew’s Hospital, London, United Kingdom

Address correspondence to: Dr. Paul J. Thornalley, Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex CO4 3SQ, UK. Phone/Fax: +44-1206-873010; thorp{at}essex.ac.uk

Received for publication August 4, 2004. Accepted for publication February 16, 2005.

The aim of this study was to define the severe deficits of protein glycation adduct clearance in chronic renal failure and elimination in peritoneal dialysis (PD) and hemodialysis (HD) therapy using a liquid chromatography-triple quadrupole mass spectrometric detection method. Physiologic proteolysis of proteins damaged by glycation, oxidation, and nitration forms protein glycation, oxidation, and nitration free adducts that are released into plasma for urinary excretion. Inefficient elimination of these free adducts in uremia may lead to their accumulation. Patients with mild uremic chronic renal failure had plasma glycation free adduct concentrations increased up to five-fold associated with a decline in renal clearance. In patients with ESRD, plasma glycation free adducts were increased up to 18-fold on PD and up to 40-fold on HD. Glycation free adduct concentrations in peritoneal dialysate increased over 2- to 12-h dwell time, exceeding the plasma levels markedly. Plasma glycation free adducts equilibrated rapidly with dialysate of HD patients, with both plasma and dialysate concentrations decreasing during a 4-h dialysis session. It is concluded that there are severe deficits of protein glycation free adduct clearance in chronic renal failure and in ESRD on PD and HD therapy.

This article has been cited by other articles:

				E. G. Gerrits, A. J. Smit, and H. J. G. Bilo AGEs, autofluorescence and renal function Nephrol. Dial. Transplant., November 25, 2008; (2008) gfn634v1. [Full Text] [PDF]

				Y. Wang, W. Beck, R. Deppisch, S. M. Marshall, N. A. Hoenich, and M. G. Thompson Differential effects of dialysis and ultrafiltrate from individuals with CKD, with or without diabetes, on platelet phosphatidylserine externalization Am J Physiol Renal Physiol, January 1, 2008; 294(1): F220 - F228. [Abstract] [Full Text] [PDF]

				G. Cohen, G. Glorieux, P. Thornalley, E. Schepers, N. Meert, J. Jankowski, V. Jankowski, A. Argiles, B. Anderstam, P. Brunet, et al. Review on uraemic toxins III: recommendations for handling uraemic retention solutes in vitro towards a standardized approach for research on uraemia Nephrol. Dial. Transplant., December 1, 2007; 22(12): 3381 - 3390. [Full Text] [PDF]

				S. Bengmark Advanced Glycation and Lipoxidation End Products-Amplifiers of Inflammation: The Role of Food JPEN J Parenter Enteral Nutr, September 1, 2007; 31(5): 430 - 440. [Abstract] [Full Text] [PDF]

				C. P. Schmitt, D. von Heyl, S. Rieger, K. Arbeiter, K. E. Bonzel, M. Fischbach, J. Misselwitz, A.-K. Pieper, F. Schaefer, and for the Mid European Pediatric Peritoneal Dialysis Reduced systemic advanced glycation end products in children receiving peritoneal dialysis with low glucose degradation product content Nephrol. Dial. Transplant., July 1, 2007; 22(7): 2038 - 2044. [Abstract] [Full Text] [PDF]

				F. Galli Protein damage and inflammation in uraemia and dialysis patients Nephrol. Dial. Transplant., July 1, 2007; 22(suppl_5): v20 - v36. [Abstract] [Full Text] [PDF]

				L. Segall and A. Covic Cardiovascular disease in haemodialysis and peritoneal dialysis: arguments pro haemodialysis Nephrol. Dial. Transplant., January 1, 2007; 22(1): 59 - 63. [Full Text] [PDF]

				J. M. Bargman Peritoneal dialysis solutions and patient survival: does wishing make it so? Nephrol. Dial. Transplant., October 1, 2006; 21(10): 2684 - 2686. [Full Text] [PDF]

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673