Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


Published ahead of print on April 6, 2005
J Am Soc Nephrol 16: 1723-1732, 2005
© 2005 American Society of Nephrology
doi: 10.1681/ASN.2004121089
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
ASN.2004121089v1
16/6/1723    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fang, T.-C.
Right arrow Articles by Poulsom, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fang, T.-C.
Right arrow Articles by Poulsom, R.

Pathophysiology of Renal Disease and Progression

Proliferation of Bone Marrow-Derived Cells Contributes to Regeneration after Folic Acid-Induced Acute Tubular Injury

Te-Chao Fang*,{dagger},{ddagger}, Malcolm R. Alison*,{dagger}, H. Terence Cook§, Rosemary Jeffery*, Nicholas A. Wright*,{dagger} and Richard Poulsom*,{dagger}

* Histopathology Unit, Cancer Research UK, London, United Kingdom; {dagger} Institute of Cell and Molecular Science, Queen Mary’s School of Medicine and Dentistry, University of London, United Kingdom; {ddagger} Division of Nephrology, Tzu Chi General Hospital, Hualien, Taiwan; and § Division of Investigative Science, Imperial College, University of London, London, United Kingdom

Address correspondence to: Prof. Richard Poulsom, Histopathology Unit, Cancer Research UK, London Research Institute, 44 Lincoln’s Inn Fields, London WC2A 3PX, UK. Phone: +44-20-7269-3438; Fax: +44-20-7269-3491; E-mail: richard.poulsom{at}cancer.org.uk

Received for publication December 15, 2004. Accepted for publication February 28, 2005.

Studies of tissue from recipients of bone marrow transplantation or organ allograft suggest that bone marrow-derived cells (BMDC) may differentiate into a variety of nonhematologic tissues, including renal tubular epithelium. The aims of this study were to examine whether BMDC contribute to recovery after acute renal injury and to assess the effects of cytokine mobilization on regeneration. Female mice (6 wk old) were lethally irradiated and transplanted with male bone marrow (BM) cells and later assigned into control, folic acid-treatment, and folic acid-treatment with granulocyte-colony stimulating factor (G-CSF), and control with G-CSF. Tritiated thymidine was given 1 h before death. Kidney sections were stained for a tubular epithelial marker, Y chromosome (in situ hybridization), periodic acid-Schiff staining, and subjected to autoradiography. Renal tubular epithelial cells in S-phase were scored as female (indigenous) or male (BM-derived). This is the first report to show that BMDC can respond by engrafting the renal tubules and undergo DNA synthesis after acute renal injury. BMDC contributed to the renal tubular epithelial cell population, although most (90%) renal tubular regeneration came from female indigenous cells. Some evidence was found for cell fusion between indigenous renal tubular cells and BMDC, but this was infrequent and the significance and consequences of cell fusion in the kidney are unresolved. G-CSF treatment nearly doubled the frequency of thymidine-labeled BM-derived tubular cells and might facilitate the recovery of renal tubular epithelium.




This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
G. Stokman, J. C. Leemans, I. Stroo, I. Hoedemaeker, N. Claessen, G. J. D. Teske, J. J. Weening, and S. Florquin
Enhanced mobilization of bone marrow cells does not ameliorate renal fibrosis
Nephrol. Dial. Transplant., February 1, 2008; 23(2): 483 - 491.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Ohnishi, S. Mizuno, and T. Nakamura
Inhibition of tubular cell proliferation by neutralizing endogenous HGF leads to renal hypoxia and bone marrow-derived cell engraftment in acute renal failure
Am J Physiol Renal Physiol, February 1, 2008; 294(2): F326 - F335.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
L. Li, P. Truong, P. Igarashi, and F. Lin
Renal and Bone Marrow Cells Fuse after Renal Ischemic Injury
J. Am. Soc. Nephrol., December 1, 2007; 18(12): 3067 - 3077.
[Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
R. Poulsom, E. I. Prodromidi, C. D. Pusey, and H. T. Cook
Cell therapy for renal regeneration--time for some joined-up thinking?
Nephrol. Dial. Transplant., December 1, 2006; 21(12): 3349 - 3353.
[Full Text] [PDF]

Home page
 Stem CellsHome page
E. I. Prodromidi, R. Poulsom, R. Jeffery, C. A. Roufosse, P. J. Pollard, C. D. Pusey, and H. T. Cook
Bone Marrow-Derived Cells Contribute to Podocyte Regeneration and Amelioration of Renal Disease in a Mouse Model of Alport Syndrome
Stem Cells, November 1, 2006; 24(11): 2448 - 2455.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. H. Little
Regrow or Repair: Potential Regenerative Therapies for the Kidney
J. Am. Soc. Nephrol., September 1, 2006; 17(9): 2390 - 2401.
[Abstract] [Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673