2008 JASN IMPACT FACTOR 7.505	HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP
advanced	CURRENT ISSUE	ARCHIVES	JASN Express	ONLINE SUBMISSION

This Article Full Text Full Text (PDF) All Versions of this Article: ASN.2005101077v1 17/11/3082    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jo, S.-K. Articles by Kim, H. K. Search for Related Content PubMed PubMed Citation Articles by Jo, S.-K. Articles by Kim, H. K.

Heat Preconditioning Attenuates Renal Injury in Ischemic ARF in Rats: Role of Heat-Shock Protein 70 on NF-B–Mediated Inflammation and on Tubular Cell Injury

Division of Nephrology, Department of Internal Medicine, Institute of Renal Disease, Korea University Hospital, Seoul, Korea

Address correspondence to: Dr. Won Yong Cho, Division of Nephrology, Department of Internal Medicine, Korea University Hospital, 126-1, 5Ka, Anam-Dong, Sungbuk-Ku, Seoul, Korea 136-705. Phone: +82-2-920-5599; Fax: +82-2-927-5344; E-mail: wonyong{at}korea.ac.kr

Received for publication October 18, 2005. Accepted for publication August 24, 2006.

Although heat preconditioning has been known to be protective in various types of injury, the precise molecular mechanism for this is unclear. Recent observations that indicate that previous heat shock has an anti-inflammatory, antiapoptotic effect led to this investigation of the in vivo effect of heat preconditioning on NF-B activation and inflammation and also on tubular cell injury in ischemic acute renal failure (ARF). Heat preconditioning provided marked functional protection and also reduced histologic evidence of tubular necrosis. Ischemia/reperfusion–induced NF-B activation was suppressed by heat preconditioning with a subsequent decrease in monocyte chemoattractant protein-1 expression and inflammatory cell infiltration. Heat preconditioning also suppressed the accumulation of phosphorylated inhibitory B (IB) with a resultant depletion of cytoplasmic IB, indicating that heat preconditioning blocked the activation of the IB kinase complex. Tubular cell apoptosis, determined by terminal deoxynucleotidyl transferase–mediated dUTP nick-end labeling staining, also was decreased by heat preconditioning, and this was accompanied by decreased caspase 3 activation. Among several heat-shock proteins (HSP), HSP-70 was induced primarily by heat preconditioning. Inhibition of HSP-70 by quercetin almost completely reversed the functional protection that was provided by heat preconditioning. These data provide evidence that HSP-70 affords protection via inhibition of NF-B–mediated inflammation and also inhibition of the cell death pathway in ischemic ARF. Further elucidation of the cytoprotective mechanism of stress proteins could facilitate new target or drug development in the treatment of ARF.

This article has been cited by other articles:

				H. Sugiura, T. Yoshida, M. Mitobe, S. Yoshida, S. Shiohira, K. Nitta, and K. Tsuchiya Klotho reduces apoptosis in experimental ischaemic acute kidney injury via HSP-70 Nephrol. Dial. Transplant., January 1, 2010; 25(1): 60 - 68. [Abstract] [Full Text] [PDF]

				E. Kansanen, H.-K. Jyrkkanen, O. L. Volger, H. Leinonen, A. M. Kivela, S.-K. Hakkinen, S. R. Woodcock, F. J. Schopfer, A. J. Horrevoets, S. Yla-Herttuala, et al. Nrf2-dependent and -independent Responses to Nitro-fatty Acids in Human Endothelial Cells: IDENTIFICATION OF HEAT SHOCK RESPONSE AS THE MAJOR PATHWAY ACTIVATED BY NITRO-OLEIC ACID J. Biol. Chem., November 27, 2009; 284(48): 33233 - 33241. [Abstract] [Full Text] [PDF]

				R. A. Zager Uremia induces proximal tubular cytoresistance and heme oxygenase-1 expression in the absence of acute kidney injury Am J Physiol Renal Physiol, February 1, 2009; 296(2): F362 - F368. [Abstract] [Full Text] [PDF]

				M. Naito, K. Bomsztyk, and R. A. Zager Renal Ischemia-Induced Cholesterol Loading: Transcription Factor Recruitment and Chromatin Remodeling along the HMG CoA Reductase Gene Am. J. Pathol., January 1, 2009; 174(1): 54 - 62. [Abstract] [Full Text] [PDF]

				Y. Yokomaku, T. Sugimoto, S. Kume, S.-i. Araki, K. Isshiki, M. Chin-Kanasaki, M. Sakaguchi, N. Nitta, M. Haneda, D. Koya, et al. Asialoerythropoietin Prevents Contrast-Induced Nephropathy J. Am. Soc. Nephrol., February 1, 2008; 19(2): 321 - 328. [Abstract] [Full Text] [PDF]

Copyright © 2009 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673