2008 JASN IMPACT FACTOR 7.505	HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP
advanced	CURRENT ISSUE	ARCHIVES	JASN Express	ONLINE SUBMISSION

This Article Full Text Full Text (PDF) All Versions of this Article: ASN.2008030310v1 20/4/831    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huang, Y. Articles by Womer, K. L. Search for Related Content PubMed PubMed Citation Articles by Huang, Y. Articles by Womer, K. L. Related Collections Related Article

Kidney-Derived Stromal Cells Modulate Dendritic and T Cell Responses

Yanfei Huang^*, Peter Johnston, Borui Zhang^*, Asif Zakari^*, Tayseer Chowdhry^*, Rachel Ruckdeschel Smith, Eduardo Marbán, Hamid Rabb^* and Karl L. Womer^*

Divisions of ^* Nephrology and Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland

Correspondence: Dr. Karl L. Womer, Ross Building 947, 720 Rutland Avenue, Baltimore, MD 21205. Phone: 410-502-3707; Fax: 410-502-5944; E-mail: kwomer1{at}jhmi.edu

Received for publication March 19, 2008. Accepted for publication November 26, 2008.

Multipotent mesenchymal stromal cells from the bone marrow ameliorate acute kidney injury through a mechanism other than transdifferentiation into renal tissue. Stromal cells exert immunoregulatory effects on dendritic and T cells, both of which are important in the pathophysiology of immune-mediated kidney injury. We hypothesized that similar cells with immunoregulatory function exist within the adult kidney. We isolated murine kidney-derived cells with morphologic features, growth properties, and an immunophenotype characteristic of mesenchymal stromal cells. These cells lacked lineage markers and could be differentiated into mesodermal cell lineages, including osteocytes and adipocytes. Furthermore, these kidney-derived cells induced the generation of bone marrow–derived dendritic cells with significantly reduced MHC II expression, increased CD80 expression, increased IL-10 production and the inability to stimulate CD4⁺ T cell proliferation in allogeneic and nominal antigen-specific cultures. Experiments in mixed and transwell cultures demonstrated that the production of soluble immune modulators, such as IL-6, was responsible for these effects on dendritic cell differentiation and maturation. Contact-dependent mechanisms, however, inhibited mitogenic T cell proliferation. In summary, kidney-derived cells may suppress inflammation in the kidney in vivo; a better understanding of their biology could have therapeutic implications in a wide variety of immune-mediated kidney diseases.

Related Article

Interfacing Kidney Stroma with Dendritic Cells

Zae Kim and Peter J. Nelson
J. Am. Soc. Nephrol. 2009 20: 685-686. [Full Text] [PDF]

This article has been cited by other articles:

				S. Agrawal, P. Gollapudi, R. Elahimehr, M. V. Pahl, and N. D. Vaziri Effects of end-stage renal disease and haemodialysis on dendritic cell subsets and basal and LPS-stimulated cytokine production Nephrol. Dial. Transplant., November 9, 2009; (2009) gfp580v1. [Abstract] [Full Text] [PDF]

				Z. Kim and P. J. Nelson Interfacing Kidney Stroma with Dendritic Cells J. Am. Soc. Nephrol., April 1, 2009; 20(4): 685 - 686. [Full Text] [PDF]

Copyright © 2009 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673