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Multiphoton Imaging Reveals Differences in Mitochondrial Function between Nephron Segments

Andrew M. Hall^*,, Robert J. Unwin, Nadeene Parker^* and Michael R. Duchen^*

^* Department of Cell and Developmental Biology and Centre for Nephrology, University College London, London, United Kingdom

Correspondence: Dr. Andrew M. Hall, Department of Cell and Developmental Biology, UCL, London WC1E 6BT, UK. Phone: +44-0-207-679-7127; Fax: +44-0-207-813-0530; E-mail: andrew.hall{at}ucl.ac.uk

Received for publication July 21, 2008. Accepted for publication January 22, 2009.

Mitochondrial dysfunction may play a role in the pathogenesis of several renal diseases. Although functional roles and metabolic demands differ among tubule segments, relatively little is known about the properties of mitochondria in different parts of the nephron. Clinically, the proximal tubule seems particularly vulnerable to mitochondrial toxicity. In this study, we used multiphoton imaging of live rat kidney slices to investigate differences in mitochondrial function along the nephron. The mitochondrial membrane potential was markedly higher in distal than proximal tubules. Inhibition of respiration rapidly collapsed the membrane potential in proximal tubules, but potential was better maintained in distal tubules. Inhibition of the F₁F_o-ATPase abolished this difference, suggesting that maintenance of potential via ATPase activity is more effective in distal than proximal tubules. Immunostaining revealed that the ratio of the expression of ATPase to IF1, an endogenous inhibitor of the mitochondrial ATPase, was lower in proximal tubules than in distal tubules. Production of reactive oxygen species was higher in proximal than distal cells, but inhibition of NADPH oxidase eliminated this difference. Glutathione levels were higher in proximal tubules. Overall, mitochondria in the proximal tubules were in a more oxidized state than those in the distal tubules. In summary, there are axial differences in mitochondrial function along the nephron, which may contribute to the pattern and pathophysiology of some forms of renal injury.

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Copyright © 2009 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673