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The E-Selectin Ligand Basigin/CD147 Is Responsible for Neutrophil Recruitment in Renal Ischemia/Reperfusion

Noritoshi Kato^*,, Yukio Yuzawa, Tomoki Kosugi^*,, Akinori Hobo^*,, Waichi Sato, Yuko Miwa^*, Kazuma Sakamoto^*, Seiichi Matsuo and Kenji Kadomatsu^*

Departments of ^* Biochemistry and Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan

Correspondence: Kenji Kadomatsu, Department of Biochemistry, Nagoya University Graduate School of Medicine, 65 Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan. Phone: +81-52-744-2060; Fax: +81-52-744-2065; E-mail: kkadoma{at}med.nagoya-u.ac.jp

Received for publication September 12, 2008. Accepted for publication February 27, 2009.

E-selectin and its ligands are essential for extravasation of leukocytes in inflammation. Here, we report that basigin (Bsg)/CD147 is a ligand for E-selectin that promotes renal inflammation in ischemia/reperfusion. Compared with wild-type mice, Bsg-deficient (Bsg^–/–) mice demonstrated striking suppression of neutrophil infiltration in the kidney after renal ischemia/reperfusion. Although E-selectin expression increased similarly between the two genotypes, Bsg^–/– mice exhibited less renal damage, suggesting that Bsg on neutrophils contribute to renal injury in this model. Neutrophils expressed Bsg with N-linked polylactosamine chains and Bsg^–/– neutrophils showed reduced binding to E-selectin. Bsg isolated from HL-60 cells bound to E-selectin, and tunicamycin treatment to abolish N-linked glycans from Bsg abrogated this binding. Furthermore, Bsg^–/– neutrophils exhibited reduced E-selectin-dependent adherence to human umbilical vein endothelial cells in vitro. Injection of labeled neutrophils into mice showed that Bsg^–/– neutrophils were less readily recruited to the kidney after renal ischemia/reperfusion than Bsg^+/+ neutrophils, regardless of the recipient's genotype. Taken together, these results indicate that Bsg is a physiologic ligand for E-selectin that plays a critical role in the renal damage induced by ischemia/reperfusion.

Copyright © 2009 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673