Journal of the American Society of Nephrology, Vol 3, 1131-1138, Copyright © 1992 by American Society of Nephrology

REGULAR ARTICLES

Vasoactive substances induce cytoskeletal changes in cultured rat glomerular epithelial cells

R Sharma, HB Lovell, TB Wiegmann and VJ Savin
Department of Medicine, University of Kansas Medical Center, Kansas City 66103.

Angiotensin II (ANG II), atrial natriuretic peptide III (ANP), and sodium nitroprusside (SNP) alter capillary hydraulic conductivity in isolated glomeruli. These agents also affect cyclic nucleotide levels of glomerular epithelial cells (GEC). ANG II increases cAMP, whereas ANP and SNP increase cGMP. The effects of these vasoactive substances on GEC cytoskeleton were tested by incubating cells from primary cultures or an established cell line with each agent. Changes in the cytoskeleton were assessed by staining for F-actin with Bodipy phallacidin and for tubulin with NBD-colcemid. Control cells exhibited short bundles of F-actin or stress fibers near the base of the cells. These were frequently arranged in parallel and occasionally appeared to radiate from the center of the cell. Microtubules were arranged in a fine network throughout the cell with increased density adjacent to the nucleus and within the nucleolus. Incubation of GEC with 10(-7) M ANG II, cholera toxin, or 8Br-cAMP for 2 at 37 degrees C resulted in rearrangement of F-actin into distinct stellate patterns with a decrease in the relative intensity of the peripheral staining, all concurrent with a fivefold increase in intracellular cAMP. The incubation of GEC with 10(-6) M ANP or 10(-7) M SNP for 2 h at 37 degrees C resulted in apparent disassembly of stress fibers, sparse and more diffuse fluorescence, and some increase of fluorescence at the periphery of the cells, all concurrent with a 10-fold increase in intracellular cGMP. Cytochalasin D incubation led to complete disassembly of actin filaments.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				S. D. Crowley, C. W. Frey, S. K. Gould, R. Griffiths, P. Ruiz, J. L. Burchette, D. N. Howell, N. Makhanova, M. Yan, H.-S. Kim, et al. Stimulation of lymphocyte responses by angiotensin II promotes kidney injury in hypertension Am J Physiol Renal Physiol, August 1, 2008; 295(2): F515 - F524. [Abstract] [Full Text] [PDF]

				D. Macconi, M. Abbate, M. Morigi, S. Angioletti, M. Mister, S. Buelli, M. Bonomelli, P. Mundel, K. Endlich, A. Remuzzi, et al. Permselective Dysfunction of Podocyte-Podocyte Contact upon Angiotensin II Unravels the Molecular Target for Renoprotective Intervention Am. J. Pathol., April 1, 2006; 168(4): 1073 - 1085. [Abstract] [Full Text] [PDF]

				M. C. Liebau, D. Lang, J. Bohm, N. Endlich, M. J. Bek, I. Witherden, P. W. Mathieson, M. A. Saleem, H. Pavenstadt, and K.-G. Fischer Functional expression of the renin-angiotensin system in human podocytes Am J Physiol Renal Physiol, March 1, 2006; 290(3): F710 - F719. [Abstract] [Full Text] [PDF]

				M. P. Winn, N. Daskalakis, R. F. Spurney, and J. P. Middleton Unexpected Role of TRPC6 Channel in Familial Nephrotic Syndrome: Does It Have Clinical Implications? J. Am. Soc. Nephrol., February 1, 2006; 17(2): 378 - 387. [Full Text] [PDF]

				S. Hoffmann, D. Podlich, B. Hahnel, W. Kriz, and N. Gretz Angiotensin II Type 1 Receptor Overexpression in Podocytes Induces Glomerulosclerosis in Transgenic Rats J. Am. Soc. Nephrol., June 1, 2004; 15(6): 1475 - 1487. [Abstract] [Full Text] [PDF]

				D. M. Attia, O. Feron, R. Goldschmeding, L. H. Radermakers, N. D. Vaziri, P. Boer, J.-L. Balligand, H. A. Koomans, and J. A. Joles Hypercholesterolemia in Rats Induces Podocyte Stress and Decreases Renal Cortical Nitric Oxide Synthesis via an Angiotensin II Type 1 Receptor-Sensitive Mechanism J. Am. Soc. Nephrol., April 1, 2004; 15(4): 949 - 957. [Abstract] [Full Text] [PDF]

				L. C. Martineau, L. I. McVeigh, B. J. Jasmin, and C. R. J. Kennedy p38 MAP kinase mediates mechanically induced COX-2 and PG EP4 receptor expression in podocytes: implications for the actin cytoskeleton Am J Physiol Renal Physiol, April 1, 2004; 286(4): F693 - F701. [Abstract] [Full Text] [PDF]

				H. Pavenstadt, W. Kriz, and M. Kretzler Cell Biology of the Glomerular Podocyte Physiol Rev, January 1, 2003; 83(1): 253 - 307. [Abstract] [Full Text] [PDF]

				G. Ding, K. Reddy, A. A. Kapasi, N. Franki, N. Gibbons, B. S. Kasinath, and P. C. Singhal Angiotensin II induces apoptosis in rat glomerular epithelial cells Am J Physiol Renal Physiol, July 1, 2002; 283(1): F173 - F180. [Abstract] [Full Text] [PDF]

				A. K. Kiemer, N. C. Weber, R. Furst, N. Bildner, S. Kulhanek-Heinze, and A. M. Vollmar Inhibition of p38 MAPK Activation via Induction of MKP-1: Atrial Natriuretic Peptide Reduces TNF-{alpha}-Induced Actin Polymerization and Endothelial Permeability Circ. Res., May 3, 2002; 90(8): 874 - 881. [Abstract] [Full Text] [PDF]

				N. ENDLICH, K. R. KRESS, J. REISER, D. UTTENWEILER, W. KRIZ, P. MUNDEL, and K. ENDLICH Podocytes Respond to Mechanical Stress In Vitro J. Am. Soc. Nephrol., March 1, 2001; 12(3): 413 - 422. [Abstract] [Full Text]

				H. Pavenstadt Roles of the podocyte in glomerular function Am J Physiol Renal Physiol, February 1, 2000; 278(2): F173 - F179. [Abstract] [Full Text] [PDF]

				H. Kurihara, N. Sunagawa, T. Kobayashi, K. Kimura, N. Takasu, and T. Shike Monoclonal antibody P-31 recognizes a novel intermediate filament-associated protein (p250) in rat podocytes Am J Physiol Renal Physiol, May 1, 1998; 274(5): F986 - F997. [Abstract] [Full Text] [PDF]

				M. Sharma, R. Sharma, Andrew. S. Greene, E. T. McCarthy, and V. J. Savin Documentation of angiotensin II receptors in glomerular epithelial cells Am J Physiol Renal Physiol, March 1, 1998; 274(3): F623 - F627. [Abstract] [Full Text] [PDF]

				A. K. Kiemer, N. C. Weber, R. Furst, N. Bildner, S. Kulhanek-Heinze, and A. M. Vollmar Inhibition of p38 MAPK Activation via Induction of MKP-1: Atrial Natriuretic Peptide Reduces TNF-{alpha}-Induced Actin Polymerization and Endothelial Permeability Circ. Res., May 3, 2002; 90(8): 874 - 881. [Abstract] [Full Text] [PDF]

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673