Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hise, M. K.
Right arrow Articles by Fontana, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hise, M. K.
Right arrow Articles by Fontana, J. A.

Journal of the American Society of Nephrology, Vol 4, 62-68, Copyright © 1993 by American Society of Nephrology

REGULAR ARTICLES

Insulin-like growth factor-I receptor and binding proteins in rat kidney after nephron loss

MK Hise, JS Lahn, ZM Shao, NM Mantzouris and JA Fontana
Department of Internal Medicine, University of Maryland Medical School, Baltimore.

Insulin-like growth factor (IGF)-binding proteins and the IGF-I receptor in rat kidney were studied to gain perspective on their potential roles in the control of renal cell mass. Thirty days after nephrectomy (UNx), membranous whole-kidney binding of IGF-I averaged 9.5 +/- 1.0 pmol/kidney, whereas binding to sham-operated controls (SNx) averaged 6.3 +/- 0.8 pmol/kidney (N = 6; P < 0.01). Scatchard analysis of IGF-I binding per milligram of protein to glomerular membranes or proximal tubule basolateral membranes (BLM) at Day 30 did not reveal significance differences in Bmax or KD between SNx and UNx; however, protein per glomerulus increased from 361 +/- 33 ng/glomerulus in SNx animals to 826 +/- 123 ng/glomerulus in UNx rats (N = 6; P < 0.002). Histomorphometrics documented an increase in proximal tubule circumference per cell and an increase in the basolateral/basement membrane ratio. IGF-I affinity labeling studies demonstrated three proteins in both glomerular membranes and proximal tubule BLM; molecular weight approximately 140,000 d, the alpha subunit of the IGF- I receptor, a protein > 200,000 d, and a protein approximately 31,000 d that was immunostained with IGF-binding protein-5 antibodies. Differences in expression between SNx and UNx were not observed at Day 30 in either glomeruli or BLM. These studies suggest that cytosolic hypertrophy of proximal nephron structures is accompanied by membrane hypertrophy with a fixed density of IGF-I receptor and IGF-binding protein-5.


This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
M. R. Hammerman
`The growth hormone–insulin-like growth factor axis in kidney re-revisited
Nephrol. Dial. Transplant., August 1, 1999; 14(8): 1853 - 1860.
[Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673