Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tanner, G. A.
Right arrow Articles by McAteer, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanner, G. A.
Right arrow Articles by McAteer, J. A.

Journal of the American Society of Nephrology, Vol 6, 1230-1241, Copyright © 1995 by American Society of Nephrology

REGULAR ARTICLES

An in vitro test of the cell stretch-proliferation hypothesis of renal cyst enlargement

GA Tanner, PF McQuillan, MR Maxwell, JK Keck and JA McAteer
Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis 46202, USA.

In renal cystic disease, fluid accumulation within cyst lumens might stretch cyst walls and in this way stimulate cell proliferation. To test this idea, the effects of mechanical stretch on Madin-Darby canine kidney cells grown as cysts in a hydrated collagen gel or as monolayers on collagen-coated Flexcell membranes were examined. The percentage of cells synthesizing DNA (labeling index) was determined by measuring bromodeoxyuridine incorporation and counting cell numbers. The distension of single cysts for 1 h by the intraluminal injection of saline failed to produce a significant increase in labeling index. The exposure of cysts for 2.5 h to 1 mM dibutyryl cAMP + 0.1 mM isobutylmethylxanthine led to a 37% increase in luminal surface area (due to stimulated fluid secretion) and a 30% increase in labeling index. The stretch (25%) of Madin-Darby canine kidney monolayers approximately doubled the labeling index between 12 and 24 h after starting the stretch. After 48 h, the cell population density was significantly increased (P < 0.001), from 41.9 +/- 0.2 (SE; N = 12) to 48.2 +/- 0.5 (N = 12) cells/10,000 microns2. The labeling index increased linearly with applied stretch, from 7.2 +/- 0.3% (N = 36) with no stretch to 16.2 +/- 1.0% (N = 6) with 30% stretch. Stretch had to be maintained for 8 h or more to produce an increase in labeling index at 18 h. No evidence was obtained for the release of a diffusible growth factor by stretched monolayers. The increase in labeling index induced by stretch was unaffected by 50 microM gadolinium, a stretch- activated channel blocker, but was abolished by 5 micrograms/mL cytochalasin B, an actin microfilament-disrupting agent. It was concluded that prolonged stretch stimulates renal epithelial cells to synthesize DNA. This supports the idea that increased wall tension in renal cysts may stimulate cell proliferation and, thereby, may contribute to cyst enlargement.


This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
B. Yang, N. D. Sonawane, D. Zhao, S. Somlo, and A. S. Verkman
Small-Molecule CFTR Inhibitors Slow Cyst Growth in Polycystic Kidney Disease
J. Am. Soc. Nephrol., July 1, 2008; 19(7): 1300 - 1310.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. R. Amura, K. S. Brodsky, B. Gitomer, K. McFann, G. Lazennec, M. T. Nichols, A. Jani, R. W. Schrier, and R. Brian Doctor
CXCR2 agonists in ADPKD liver cyst fluids promote cell proliferation
Am J Physiol Cell Physiol, March 1, 2008; 294(3): C786 - C796.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Hufnagel, A. A. Teleman, H. Rouault, S. M. Cohen, and B. I. Shraiman
On the mechanism of wing size determination in fly development
PNAS, March 6, 2007; 104(10): 3835 - 3840.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S. Omori, M. Hida, H. Fujita, H. Takahashi, S. Tanimura, M. Kohno, and M. Awazu
Extracellular Signal-Regulated Kinase Inhibition Slows Disease Progression in Mice with Polycystic Kidney Disease
J. Am. Soc. Nephrol., June 1, 2006; 17(6): 1604 - 1614.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
J. Galle, M. Loeffler, and D. Drasdo
Modeling the Effect of Deregulated Proliferation and Apoptosis on the Growth Dynamics of Epithelial Cell Populations In Vitro
Biophys. J., January 1, 2005; 88(1): 62 - 75.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. P. Yang, A. S. Woolf, H. T. Yuan, R. J. Scott, R. A. Risdon, M. J. O'Hare, and P. J. D. Winyard
Potential Biological Role of Transforming Growth Factor-{beta}1 in Human Congenital Kidney Malformations
Am. J. Pathol., November 1, 2000; 157(5): 1633 - 1647.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
L. P. SULLIVAN, D. P. WALLACE, and J. J. GRANTHAM
Epithelial Transport in Polycystic Kidney Disease
Physiol Rev, October 1, 1998; 78(4): 1165 - 1191.
[Abstract] [Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673