Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Salahudeen, A.
Right arrow Articles by Roberts, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salahudeen, A.
Right arrow Articles by Roberts, J., 2nd

Journal of the American Society of Nephrology, Vol 6, 1300-1303, Copyright © 1995 by American Society of Nephrology

REGULAR ARTICLES

Hydrogen peroxide induces 21-aminosteroid-inhibitable F2-isoprostane production and cytolysis in renal tubular epithelial cells

A Salahudeen, K Badr, J Morrow and J Roberts 2nd
Department of Medicine, University of Mississippi Medical Center, Jackson 39216-4505, USA.

F2-isoprostanes are the newly identified reactive oxygen species- catalyzed peroxidation products of arachidonate. The infusion of these prostaglandin F2-like prostanaoids into the rat kidney induces profound parallel reductions in RBF and GFR, suggesting that these metabolites may be partly responsible for the hemodynamic alterations seen in free radical-linked acute renal injury models. The present study examined directly in renal proximal tubular (LLC-PK1) cells whether hydrogen peroxide, a reactive oxygen species implicated in many models of acute renal injury, induces F2-isoprostane production and whether its production can be inhibited by the recently synthesized lipid peroxidation inhibitor 21-aminosteroid (lazaroid U-74389G). The incubation of LLC-PK1 cell layers with hydrogen peroxide for 3 h resulted in a dose-related six-fold increase in F2-isoprostane production, measured by the gas chromatographic-mass spectroscopic method. The preincubation of cells with 21-aminosteroid prevented hydrogen peroxide-induced F2-isoprostane production, a finding also demonstrable with other lipid peroxidation inhibitors, e.g., 2-methyl aminochroman (U-83836E) and diphenyl-p-phenylenediamine. Besides inhibiting isoprostane production, 21-aminosteroid reduced hydrogen peroxide-induced lipid degradation and peroxidation, and protected the cells against hydrogen peroxide-induced cytolysis. The novel finding that hydrogen peroxide induces 21-aminosteroid-inhibitable F2- isoprostane production in renal epithelial cells supports the in vivo report that its levels are elevated in reactive oxygen species-linked renal injury models such as ischemia-reperfusion. Besides direct cell injury, lipid peroxidation by generating F2-isoprostanes may further contribute to renal dysfunction through a vasoconstrictive mechanism. Thus, the inhibition of excess F2-isoprostane production may be one of the additional mechanisms, besides cytoprotection, by which antioxidants ameliorate renal dysfunction in experimental models of acute renal injury.


This article has been cited by other articles:


Home page
 Eur Heart JHome page
A. Svatikova, R. Wolk, L. O. Lerman, L. A. Juncos, E. L. Greene, J. P. McConnell, and V. K. Somers
Oxidative stress in obstructive sleep apnoea
Eur. Heart J., November 2, 2005; 26(22): 2435 - 2439.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. D. Imig
Eicosanoid regulation of the renal vasculature
Am J Physiol Renal Physiol, December 1, 2000; 279(6): F965 - F981.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
X. Hou, F. Gobeil Jr, K. Peri, G. Speranza, A. M. Marrache, P. Lachapelle, J. Roberts II, D. R. Varma, S. Chemtob, and E. F. Ellis
Augmented Vasoconstriction and Thromboxane Formation by 15-F2t-Isoprostane (8-Iso-Prostaglandin F2{alpha}) in Immature Pig Periventricular Brain Microvessels • Editorial Comment
Stroke, February 1, 2000; 31(2): 516 - 524.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Barlow, P. A. Dennery, M. K. Shigenaga, M. A. Smith, J. D. Morrow, L. J. Roberts II, A. Wynshaw-Boris, and R. L. Levine
Loss of the ataxia-telangiectasia gene product causes oxidative damage in target organs
PNAS, August 17, 1999; 96(17): 9915 - 9919.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
I. Lahaie, P. Hardy, X. Hou, H. Hassessian, P. Asselin, P. Lachapelle, G. Almazan, D. R. Varma, J. D. Morrow, L. J. Roberts II, et al.
A novel mechanism for vasoconstrictor action of 8-isoprostaglandin F2alpha on retinal vessels
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 1998; 274(5): R1406 - R1416.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. F. Reckelhoff, V. Kanji, L. C. Racusen, A. M. Schmidt, S. Du Yan, J. Morrow, L. J. Roberts II, and A. K. Salahudeen
Vitamin E ameliorates enhanced renal lipid peroxidation and accumulation of F2-isoprostanes in aging kidneys
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 1998; 274(3): R767 - R774.
[Abstract] [Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673