Journal of the American Society of Nephrology
2007 JASN IMPACT FACTOR 7.111 HOME   AUTHOR INFO   EDITORIAL BOARD   SUBSCRIBE   FEEDBACK   ALERTS   HELP 
    advanced
CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION


This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pahl, M. V.
Right arrow Articles by Ding, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pahl, M. V.
Right arrow Articles by Ding, Y.

Journal of the American Society of Nephrology, Vol 7, 2249-2253, Copyright © 1996 by American Society of Nephrology

REGULAR ARTICLES

Antithrombin III inhibits mesangial cell proliferation

MV Pahl, ND Vaziri, F Oveisi, J Wang and Y Ding
Department of Medicine, University of California, Irvine Medical Center 92668, USA.

Thrombin stimulates and heparin and heparan sulfate inhibit mesangial cell proliferation. In addition, heparin has been shown to inhibit thrombin-stimulated smooth muscle cell proliferation. The anticoagulant action of heparin is mediated by antithrombin III. This study investigated whether heparin's antiproliferative action is also mediated by antithrombin III. To this end, the effect of antithrombin III on thrombin-stimulated mesangial cell growth was examined. As expected, thrombin stimulated DNA synthesis and cell growth in cultured human mesangial cells. The effect of thrombin on DNA synthesis and mesangial cell proliferation was inhibited by standard heparin and antithrombin III, separately or together. The magnitude of the inhibitory action of antithrombin III was equal to that of equimolar concentrations of heparin and that observed with the combination of the two. Experiments carried out in serum (hence antithrombin III)-free medium revealed that heparin's inhibitory effects are independent of antithrombin III. It was concluded that antithrombin III, an endogenous inhibitor of thrombin's coagulant activity, is an equally effective inhibitor of thrombin's mitogenic action.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
E. P. Girardin, S. HajMohammadi, B. Birmele, A. Helisch, N. W. Shworak, and A. I. de Agostini
Synthesis of Anticoagulantly Active Heparan Sulfate Proteoglycans by Glomerular Epithelial Cells Involves Multiple 3-O-Sulfotransferase Isoforms and a Limiting Precursor Pool
J. Biol. Chem., November 11, 2005; 280(45): 38059 - 38070.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
K. Shirato, H. Osawa, M. Kaizuka, N. Nakamura, T. Sugawara, M. Nakamura, M. Tamura, H. Yamabe, and K. Okumura
Thrombin stimulates production of fibronectin by human proximal tubular epithelial cells via a transforming growth factor-{beta}-dependent mechanism
Nephrol. Dial. Transplant., November 1, 2003; 18(11): 2248 - 2254.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
Y. Ding and N. D. Vaziri
Calcium Channel Blockade Enhances Nitric Oxide Synthase Expression by Cultured Endothelial Cells
Hypertension, October 1, 1998; 32(4): 718 - 723.
[Abstract] [Full Text] [PDF]


HOME CURRENT ISSUE ARCHIVES JASN Express ONLINE SUBMISSION AUTHOR INFO
EDITORIAL BOARD SUBSCRIBE FEEDBACK ALERTS HELP

Copyright © 2008 by the American Society of Nephrology. Online ISSN: 1533-3450 Print ISSN: 1046-6673