Received August 15, 2007
Accepted on February 2, 2008

BASIC RESEARCH

Acute Kidney Injury Leads to Inflammation and Functional Changes in the Brain

Manchang Liu ^*1, Yideng Liang , Srinivasulu Chigurupati , Justin D. Lathia , Mikhail Pletnikov , Zhaoli Sun , Michael Crow ^*, Christopher A. Ross , Mark P. Mattson , and Hamid Rabb ^*

Departments of *Medicine, Psychiatry and Behavioral Sciences, and Surgery, Johns Hopkins University School of Medicine, and Laboratory of Neurosciences, National Institute on Aging Intramural Research Program, Baltimore, Maryland

¹ To whom correspondence should be addressed. E-mail: manchang{at}jhmi.edu.

	Abstract

Although neurologic sequelae of acute kidney injury (AKI) are well described, the pathogenesis of acute uremic encephalopathy is poorly understood. This study examined the short-term effect of ischemic AKI on inflammatory and functional changes of the brain in mice by inducing bilateral renal ischemia for 60 min and studying the brains 24 h later. Compared with sham mice, mice with AKI had increased neuronal pyknosis and microgliosis in the brain. AKI also led to increased levels of the proinflammatory chemokines keratinocyte-derived chemoattractant and G-CSF in the cerebral cortex and hippocampus and increased expression of glial fibrillary acidic protein in astrocytes in the cortex and corpus callosum. In addition, extravasation of Evans blue dye into the brain suggested that the blood-brain barrier was disrupted in mice with AKI. Because liver failure also leads to encephalopathy, ischemic liver injury was induced in mice with normal renal function; neuronal pyknosis and glial fibrillary acidic protein expression were not increased, suggesting differential effects on the brain depending on the organ injured. For evaluation of the effects of AKI on brain function, locomotor activity was studied using an open field test. Mice subjected to renal ischemia or bilateral nephrectomy had moderate to severe declines in locomotor activity compared with sham-operated mice. These data demonstrate that severe ischemic AKI induces inflammation and functional changes in the brain. Targeting these pathways could reduce morbidity and mortality in critically ill patients with severe AKI.