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Figure 6. Effect of doxycycline treatment on mHGF mRNA expression in DT-HGF mice and renal function and survival rates after bilateral ischemia in control mice and DT-HGF mice. (A) RNase protection assay was performed as mentioned in Figures 2 and 4 with total kidney RNA from control (Teto-HGF), DT-HGF, and doxycycline-treated DT-HGF and control (Teto-HGF) littermates. Doxycycline (20 mg/L) dissolved in 5% sucrose was added to the drinking water for the period of time described in the figure. Murine HGF mRNA expression levels in the kidney of DT-HGF mice decrease time-dependently after 7 d of doxycycline administration, reaching basal expression levels similar to control mice. (B) Doxycycline (20 mg/L) was added to the drinking water for 7 d presurgery and during the reperfusion period in the renal ischemia studies. Creatinine was measured in plasma obtained from doxycycline-treated control (Teto-HGF) and DT-HGF mice before the surgery and 48 h after bilateral ischemia. Plasma creatinine in doxycycline-treated DT-HGF mice 48 h after ischemia/reperfusion was significantly increased (*P < 0.05) compared with the presurgery values and was similar to the level in doxycycline-treated control mice. (C) The survival rate after the ischemia/reperfusion process was identical in doxycycline-treated DT-HGF mice (n = 8) and in the doxycycline-treated control (Teto-HGF) mice (n = 12).
