Article Figures & Data
Figures
- Figure 1.
Survival rate as a function of the time in sham-operated rats (—) or rats subjected to 75 min of bilateral clamping of the renal pedicles and treated with vehicle ([UNK]) or insulin-like growth factor I (IGF-I; ○).
- Figure 2.
Mean (±SEM) values of daily serum urea nitrogen (SUN) in sham-operated rats (—) or rats subjected to 75 min of bilateral clamping of the renal pedicles and treated with vehicle ([UNK]) or IGF-I (○).
- Figure 3.
Representative hematoxylin and eosin—stained renal sections from untreated (A) and IGF-I—treated (B) rats, illustrating more severe lesions of acute tubular necrosis in treated rats. Neutrophil infiltration in two representative kidney sections of corticomedullary and medullary areas of untreated (C, E) and IGF-I—treated (D, F) rats, respectively. Infiltration of neutrophils, seen as deep-blue dots, clearly is more prominent in the treated rats, particularly at the medulla. Magnification, ×280.
- Figure 4.
Expression of IGF-I mRNA in kidney tissue of the three groups of rats. Sham, sham-operated rats; ARF, rats with acute renal failure treated with vehicle; ARF+IGF-I, rats with acute renal failure treated with IGF-I; a, significantly different from Sham group. Bar graph shows IGF-I mRNA levels (mean ± SEM) quantified as described in the Materials and Methods section. On the bottom, a representative RNase protection assay experiment is shown.
- Figure 5.
Expression of IGF-I receptor (IGF-I/R) mRNA in kidney tissue of the three groups of rats. Sham, sham-operated rats; ARF, rats with acute renal failure treated with vehicle; ARF+IGF-I, rats with acute renal failure treated with IGF-I; a, significantly different from Sham group. Bar graph shows IGF-I/R mRNA levels (mean ± SEM) quantified as described in the Materials and Methods section. On the bottom, a representative RNase protection assay experiment is shown.
Tables
- Table 1.
Concentrations (±SEM) of serum urea nitrogen and creatinine in the three groups of ratsa
Group Serum Urea Nitrogen (mg/dl) Serum Creatinine (mg/dl) Day 0 Day 1 Day 2 Day 0 Day 1 Day 2 aSham, sham-operated rats; ARF, rats with acute renal failure treated with vehicle; ARF+IGF-I, rats with acute renal failure treated with insulin-like growth factor I (IGF-I). bSignificantly different (P < 0.05) from the Sham group. Sham 12 ± 1 11 ± 0 10 ± 1 0.2 ± 0.0 0.2 ± 0.0 0.2 ± 0.0 ARF 11 ± 0 114 ± 7b 171 ± 14b 0.2 ± 0.0 2.1 ± 0.2b 3.5 ± 0.3b ARF+IGF-I 11 ± 0 123 ± 7b 173 ± 14b 0.2 ± 0.0 2.5 ± 0.2b 2.8 ± 0.3b Group Score BrdU-LI (% of Cells) aData are ± SEM from five rats per group. BrdU-LI, 5-bromo-2′-deoxyuridine labeling index. bSignificantly different from Sham group. cSignificantly different from ARF. Sham 0.0 ± 0.0 0.4 ± 1.7 ARF 1.3 ± 0.2b 10.3 ± 2.6b ARF+IGF-I 1.9 ± 0.1b,c 12.0 ± 1.9b - Table 3.
Number of neutrophils (neutrophils/mm2) and macrophages (macrophages/mm2) at the corticomedullary junction and at the renal medulla in the three groups of rats killed 48 h after the ischemic insulta
Group Corticomedullary Junction Renal Medulla Neutrophils/mm2 Macrophages/mm2 Neutrophils/mm2 Macrophages/mm2 aData are ± SEM from five rats per group. bSignificantly different from Sham group. cSignificantly different from ARF. Sham 0.2 ± 0.0 0.7 ± 0.6 0.8 ± 0.4 0.3 ± 0.1 ARF 37.2 ± 4.1b 28.6 ± 9.4b 42.1 ± 9.6b 1.4 ± 0.1b ARF+IGF-I 52.3 ± 5.2b,c 27.8 ± 5.0b 172.5 ± 30.0b,c 1.7 ± 0.4b
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