Immunology and Pathology

Lipoxins, Aspirin-Triggered Epi-Lipoxins, Lipoxin Stable Analogues, and the Resolution of Inflammation: Stimulation of Macrophage Phagocytosis of Apoptotic Neutrophils In Vivo

Siobhan Mitchell, Graham Thomas, Killeen Harvey, David Cottell, Keira Reville, Giovanni Berlasconi, Nicos A. Petasis, Lars Erwig, Andrew J. Rees, John Savill, Hugh R. Brady and Catherine Godson
JASN October 2002, 13 (10) 2497-2507; DOI: https://doi.org/10.1097/01.ASN.0000032417.73640.72

Abstract

ABSTRACT. Lipoxins (LX) are eicosanoids with antiinflammatory activity in glomerulonephritis (GN) and inflammatory diseases, hypersensitivity, and ischemia reperfusion injury. It has been demonstrated that LXA4 stimulates non-phlogistic phagocytosis of apoptotic polymorphonuclear neutrophils (PMN) by monocyte-derived macrophages (Mφ) in vitro, suggesting a role for LX as endogenous pro-resolution lipid mediators. It is here reported that LXA4, LXB4, the aspirin-triggered LX (ATL) epimer, 15-epi-LXB4, and a stable synthetic analogue 15(R/S)-methyl-LXA4 stimulate phagocytosis of exogenously administered excess apoptotic PMN by macrophages (Mφ) in vivo in a classic model of acute inflammation, namely thioglycollate-induced peritonitis. Significant enhancement of phagocytosis in vivo was observed with 15-min exposure to LX and with intraperitoneal doses of LXA4, LXB4, 15(R/S)-methyl-LXA4, and 15-epi-LXB4 of 2.5 to 10 μg/kg. Non-phlogistic LX-stimulated phagocytosis by Mφ was sensitive to inhibition of PKC and PI 3-kinase and associated with increased production of transforming growth factor–β1 (TGF-β1). LX-stimulated phagocytosis was not inhibited by phosphatidylserine receptor (PSR) antisera and was abolished by prior exposure of Mφ to β1,3-glucan, suggesting a novel Mφ-PMN recognition mechanism. Interestingly, the recently described peptide agonists of the LXA4 receptor (MYFINITL and LESIFRSLLFRVM) stimulated phagocytosis through a process associated with increased TGF-β1 release. These data provide the first demonstration that LXA4, LXB4, ATL, and LX stable analogues rapidly promote Mφ phagocytosis of PMN in vivo and support a role for LX as rapidly acting, pro-resolution signals in inflammation. Engagement of the LXR by LX generated during cell-cell interactions in inflammation and by endogenous LXR peptide agonists released from distressed cells may be an important stimulus for clearance of apoptotic cells and may be amenable to pharmacologic mimicry for therapeutic gain.E-mail: cgodson@mater.ie

Rapid, efficient and tightly regulated recruitment and clearance of polymorphonuclear neutrophil (PMN) at sites of inflammation are essential components of effective host defense. Evidence from in vitro models and from histopathology suggests that tissue damage mediated by PMN is limited by apoptosis and subsequent phagocytosis of the apoptotic PMN by macrophages (Mφ) and “nonprofessional” phagocytes (1). A direct role for PMN in tissue injury in inflammation and ischemia reperfusion injury of the kidney and other organs is well established (2). Impaired clearance of apoptotic cells by Mφ has been implicated in the pathogenesis of chronic inflammatory conditions, including glomerulonehritis (GN) and systemic lupus erythematosus (SLE) (3). The endogenous signals that promote clearance of apoptotic PMN from an inflammatory focus are still being defined. By dissecting out the mediator systems that regulate this process, it may be possible to design new pro-resolution strategies for inflammatory diseases.

Lipoxins (LX), an acronym for lipoxygenase interaction products, are endogenously produced eicosanoids with anti-inflammatory actions in GN and other diseases (4). LX are formed principally by transcellular metabolism initiated by sequential oxygenation of arachidonic acid by either 15- and 5-lipoxygenases or 5- and 12-lipoxygenases (5). Lipoxin biosynthesis has been demonstrated in a range of inflammatory diseases, including experimental immune complex glomerulonephritis in rats and mice (6,8,9). Platelet-PMN biosynthetic interactions provide the dominant pathway for LX formation in the initial stages of immune complex–mediated glomerulonephritis and are likely superseded by leukocyte interactions with cytokine-activated resident tissue cells as the inflammatory process evolves (9,10). LX were originally proposed to act as “braking signals” in inflammation on the basis of their ability to inhibit neutrophil chemotaxis, adhesion, and transmigration across endothelia and epithelia (reviewed in reference 4). LXA4 and LXB4 inhibit LTB4-induced PMN chemotaxis and PMN-endothelial cell adhesion supported by CD11/CD18 integrins (7). LXA4 and LXB4 also attenuate LTC4- and LTD4-triggered endothelial hyperadhesiveness for PMN, in part by inhibiting mobilization of P-selectin from Weibel Palade bodies to the endothelial cell surface (6). More recently, the repertoire of antiinflammatory actions of LX has been expanded to include attenuation of cytokine and chemokine release (11,12), synoviocyte metalloproteinases (13), eosinophil trafficking (14), inhibition of mesangial cell proliferation (15), and stimulation of phagocytosis of apoptotic PMN in vitro (16).

Lipoxin A4 [(5S,6R,15S)-trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid] and its positional isomer lipoxin B4 [(5S,14R,15S)-trihydroxy-6,8,12-trans-10-cis-eicosatetraenoic acid] are the two principal species formed in mammalian cells (reviewed in reference 4). The rapid metabolic degradation of native LX by dehydrogenation at C15 and possibly ω-oxidation at C20 has prompted the design and synthesis of stable analogues, which retain many of the properties of the native LX, including inhibition of PMN adhesion and transmigration (17,4). In a cytokine primed milieu, the catalytic activity of aspirin-acetylated cyclooxygenase type 2 (COX-2) is shifted from a cyclooxygenase to 15R lipoxygenase. In this context, 15(R)-HETE is produced and can then be metabolized to 15 epimers of LXA4 and LXB4 during cell-cell interactions (18). These aspirin-triggered lipoxins (ATL) also possess antiinflammatory activity (19,20).

Here we demonstrate that LX, ATL, 15-epi-LXB4, and the stable synthetic LX analogue 15(R/S)-methyl-LXA4 promote Mφ phagocytosis of apoptotic PMN in vivo. We expand on the signal transduction pathways mediating LX-stimulated phagocytosis and further define the adhesive requirements necessary for efficient phagocytosis. We demonstrate that recently described peptide agonists of the lipoxin receptor (ALXR) can also promote non-phlogistic phagocytosis of apoptotic PMN associated with transforming growth factor–β1 (TGF-β1) release. These data expand on the antiinflammatory repertoire of LX and provide the first evidence of rapid regulation of PMN clearance by endogenous lipid mediators in vivo. This raises the possibility that such a role may be played not only by lipoxygenase-derived lipid mediators but also by necrotactic peptides released by dying cells. The data suggest that it may be possible to actively promote the resolution of inflammatory disease by harnessing the system pharmacologically.

Materials and Methods

Materials

RPMI 1640 and phosphate-buffered saline (PBS) were obtained from Life Technologies (Grand Island, NY). LXA4 and LXB4 were obtained from Cascade Biologicals (Berkshire, UK). 15(R/S)-methyl-LXA4, 16-phenoxy-LXA4 and the aspirin-triggered 15-epi-LXB4 were prepared by total organic synthesis (21). CM-orange (CMO, cell tracker orange) was from Molecular Probes (Oregon, US). Rat cytokines tumor necrosis factor–α (TNF-α), TGF-β, interferon-γ(IFN-γ), and TGF-β1 and MCP-1 human enzyme-linked immunosorbent assay (ELISA) kits were obtained from R&D Systems (Oxon, UK). The anti-CD36 monoclonal antibody SMφ (IgM) was provided by Dr. Nancy Hogg (ICRF, London) (22). Anti-αvβ3 mouse monoclonal antibody (23C6) was purchased from Serotec (Oxford, UK). FITC-conjugated anti-CD36 (FA6–152) and anti-αvβ3 (anti-CD51/61) (AMF-7) monoclonal antibodies were from Beckman Coulter (Luton, UK). Phosphatidylserine receptor (PSR) antisera were a gift from Dr. Valerie Fadok (National Jewish Medical and Research Center, Denver, CO) (23);isotype-matched control IgM was obtained from Calbiochem (Nottingham, UK). β1,3-glucan was purchased from Accurate Chemicals (Westbury, NY). The MHC-binding peptide (MYFINILTL) (MHC bp) and MMK-1 peptide (LESIFRSLLFRVM) were gifts from Prof. Charles N. Serhan (Brigham and Women’s Hospital, Harvard Medical School, Boston, MA.) (19). All other chemicals were obtained from Sigma (Poole, UK).

Human Leukocyte Preparation and Culture

Human monocytes and PMN were isolated from peripheral venous blood drawn from healthy volunteers who had provided informed written consent subsequent to approval from institutional ethics committees. PMN were isolated by density gradient centrifugation and dextran sedimentation and aged for 24 h; apoptosis was verified by flow cytometry as previously documented (16). Mφ were prepared from monocytes collected over Ficoll-Paque as previously reported (16). Adherent monocytes were cultured for 5 to 7 d in RPMI supplemented with 10% autologous serum and 1% penicillin-streptomycin.

Mφ Phagocytosis of Apoptotic PMN In Vitro

Mφ were treated with reagents as indicated and washed with RPMI 1640 before coincubation with aged PMN in 24-well tissue culture plates (4 × 106 PMN/ml of RPMI per well) at 37°C for 30 min. Phagocytosis was assayed by myeloperoxidase (MPO) staining of co-cultures fixed with 2.5% glutaraldehyde as previously reported (16). Mφ were routinely negative for peroxidase staining. For each experiment, the number of Mφ containing one or more PMN in at least five fields (minimum of 400 cells) was expressed as a percentage of the total number of Mφ in duplicate wells. Phagocytosis of apoptotic PMN was confirmed by electron microscopy.

Electron Microscopy

The coincubation cultures was washed with PBS, detached, and centrifuged at 500 ×g for 15 min. The cell pellet was fixed in 2.5% glutaraldehyde in PBS for 1 h at room temperature. Glutaraldehyde was removed, and the pellet was washed three times in 6.8% sucrose/PBS. One percent osmium tetraoxide was then added for 1 h at room temperature before a washing step with 6.8% sucrose/PBS. The pellet was then dehydrated in ascending grades of ethanol and embedded in epoxy resin at 60°C. For light microscopy, 1-μm sections were cut with a glass knife and stained with toluene blue. Selected areas were trimmed, and ultrathin sections were cut at 50 nm using a diamond knife; sections were picked up on 100 mesh copper grids and stained with uranyl acetate and lead citrate. These were examined and photographed in a JOEL 2000 EX Temscan at 80KV and using an objective aperture of 100 μm.

Inhibitor and Antibody Studies

Mφ were washed with RPMI 1640 before treatments with the stable cell-permeable cAMP analogue 8-Br-cAMP (2 mM) (24), the protein kinase A inhibitor Rp-cAMP (100 μM) (16), the PI 3-kinase inhibitor LY 294002 (10 μM) (15), the PKC inhibitor GF 109203X (10 μM) (15), the MAP kinase inhibitor PD 98059 (10 μM) (15), or appropriate vehicles for 15 min at 37°C before stimulation with LXA4 or LXB4 (1 nM; 15 min; 37°C). The treated cells were washed with RPMI 1640 before coincubation with aged PMN and phagocytosis assayed as described above.

Antibody blockade of cell surface adhesion molecules was affected by exposure of Mφ to anti-CD51/61 mAb (20 μg/ml) and anti-CD36 mAb (SMφ) ascitic fluid (22) (125 μl/ml) on ice for 20 min. Mφ were then treated with LX (1nM; 15 min; 37°C) or control before coincubation with apoptotic PMN for 30 min, and phagocytosis was assayed as above.

In experiments investigating the role of the phosphatidylserine receptor (PSR), Mφ were treated with PSR antisera (200 μg/ml) and isotype control human IgM on ice for 30 min before treatment with LX and subsequent coincubation with PMN (23). Phagocytosis was assessed by staining the co-cultures for MPO activity as above.

Induction of the PS Recognition System in Mφ

Treatment of Mφ with β1,3-glucan confers PSR dependence on Mφ phagocytosis of apoptotic PMN (25). On day 5, human Mφwere treated with β1,3-glucan (25 μg/ml) for 48 h before washing twice with RPMI before treatments with LX assay of phagocytosis.

Rat Bone Marrow–Derived Macrophages: Isolation and Cell Culture

Rat bone marrow–derived Mφ (BMDM) were isolated by standard procedures (26). Briefly, bone marrow cells from the dissected femurs of male Sprague-Dawley rats were flushed with complete medium through a 25-gauge needle to form a single-cell suspension. Cells were cultured in 75-mm tissue culture flasks in Dulbecco’s modified Eagle medium (DMEM) containing 2 mM glutamine, 10% fetal calf serum (FCS), 1% penicillin-streptomycin, and 10% conditioned medium from L929 cells as a source of macrophage-colony stimulating factor (M-CSF). After 7 d, cells were plated into 24-well plates at a concentration of 5 ×105 cells/well and rested for 24 h in M-CSF–free medium. Cells were stimulated with murine TNF-α (10 ng/ml for 24 h), IFN-γ/TNF-α (10 ng/ml for 4 h and 10 ng/ml for 20 h), or TGF-β (5 ng/ml for 24 h) before LXA4 treatment (1 nM for 15 min). Phagocytosis of aged human PMN was assayed as above.

Assay of TGF-β1 and MCP-1 Release from Mφ

TGF-β1 and MCP-1 were assayed in supernatants from co-cultures of PMN-Mφ by ELISA according to the manufacturer’s instruction (R&D systems).

Assessment of LX-Stimulated Mφ Phagocytosis of Apoptotic PMN In Vivo

Ten-week-old BALB/C mice were housed and treated under UK Home Office-approved conditions in University of Edinburgh, Faculty of Medicine animal facility. An enriched population of inflammatory Mφ was induced in the peritoneal cavity of groups of male BALB/C adult mice by a single intraperitoneal injection of 1 ml of 3% (wt/vol) thioglycollate 5 d before assay (3). On day 4, human PMN were isolated from peripheral blood of healthy volunteers. PMN were labeled with CM-orange (CMO, cell tracker orange) and aged in culture for 24 h to render a portion apoptotic. LXA4, LXB4, 15(R/S)-methyl-LXA4, 16-phenoxy-LXA4, and 15-epi-LXB4 were administered intraperitoneally 15 min before instillation of a bolus of labeled, apoptotic PMN (approximate 3:1 ratio of PMN to Mφ) (3). After 30 min, mice were sacrificed and peritoneal cells were harvested by lavage. Cytospin preparations of the lavages were fixed, and the number of Mφcontaining ingested CMO-positive, apoptotic PMN was determined by fluorescence microscopy and expressed relative to total number of Mφrecovered.

Statistics

Statistical significance was determined by t tests or one-way ANOVA where appropriate.

Results

Both Lipid and Peptide Agonists for LX Receptors Stimulate Non-Phlogistic Phagocytosis of Apoptotic PMN

Figure 1A shows LX-stimulated Mφ phagocytosis of apoptotic PMN as demonstrated by electron microscopy. As previously reported for LXA4 in vitro (16), LXB4 stimulated phagocytosis of apoptotic PMN in this model system, indicating that this pro-resolution effect is shared by the two major LX generated endogenously in mammalian systems. LXA4 and LXB4 demonstrated similar dose-response relationships with an EC50 of 0.5 × 10−9 M and a maximum stimulation observed at 1 nM (Figure 1B). Interestingly, additivity was not observed with simultaneous exposure of Mφ to LXA4 and LXB4 (data not shown), indicating the possibility of a common signaling pathway resulting in enhanced phagocytosis. Aspirin acetylation of cyclooxygenase-2 (COX-2) promotes the formation of 15 epi-LX in the context of neutrophil-endothelial cell interactions. The aspirin-triggered epi lipoxins (ATL) are implicated in the antiinflammatory actions of aspirin in vivo. In this study, the ATL 15 epi-LXA4 also stimulated macrophage phagocytosis (Figure 1B). Recent data indicate that, in addition to LXA4 and the stable synthetic analogues binding to the ALXR, specific peptides can also bind and elicit responses (19,27). We assessed the effects of such agents on stimulation of Mφ phagocytosis of apoptotic PMN. Treatment of Mφ for 15 min with either MHC bp or MMK-1 at 1 nM significantly enhanced phagocytosis (2-fold and 1.7-fold, respectively) in vitro, and these actions were not additive with LXA4 (Figure 1C) when used at a suboptimal concentration (0.5 nM)

Figure

Figure 1. Lipoxins (LX), aspirin-triggered LX (ATL), and peptide agonists promote non-phlogistic macrophage (Mφ) phagocytosis of apoptotic polymorphonuclear neutrophils (PMN). (A) Mφ were treated with LXA4 (1nM for 15 mins) before coincubation with aged PMN for 30 min. Electron microscopy was performed as described in Materials and Methods. (B) LXB4 and the aspirin-triggered 15-epi-LXB4 stimulate Mφphagocytosis. Mφ were treated with LXB4 (1 nM) or 15-epi-LXB4 (1 ×10−11 M) for 15 min before coincubation with aged PMN for 30 min. Data are fold induction ± SEM (n = 4);*P < 0.05 versus control. (C) Peptide agonists of LXA4 receptor (ALXR) stimulate Mφphagocytosis. Mφ were treated with MHC bp or MMK-1 synthetic peptide (1 nM for 15 min) alone or in combination with LXA4 (1 nM) for 15 min before coincubation with aged PMN for 30 min. Data are mean ± SEM (n = 4);*P < 0.05 versus control. (D) LXA4 and peptide agonists stimulate the release of transforming growth factor–β1 (TGF-β1). Mφ were treated with LXA4, MHC bp, and MMK-1 (1 nM) for 15 min at 37°C before coincubation with apoptotic PMN. After 30 min, supernatants were collected and TGF-β1 production was measured by enzyme-linked immunosorbent assay (ELISA). Data are expressed as mean TGF-β1 production (pg/ml) ± SD. (E) LXA4 and peptide agonists do not provoke release of MCP-1. Mφ were treated with LXA4, MHC bp, and MMK-1 (1 nM) for 15 min at 37°C before coincubation with apoptotic PMN. After 30 min, supernatants were collected and MCP-1 production was measured by ELISA. Data are expressed as mean MCP-1 production (pg/ml) ±SD.

In contrast to phagocytosis of opsonized particles, non-phlogistic Mφ phagocytosis of apoptotic PMN may contribute to the resolution of inflammation, being characteristically coupled with TGF-β1 release and not associated with release of proinflammatory cytokines such as IL-8 and MCP-1 (28). To investigate whether peptide and LX-mediated phagocytosis might promote the resolution of inflammation, Mφ were treated with LXA4, MHC bp, and MMK-1 (1 nM for 15 min) before coincubation with apoptotic PMN, and TGF-β1 and MCP-1 production was assayed by ELISA. LXA4 treatment induced a sixfold increase in TGF-β1 production (Figure 1D). Time course analysis of TGF-β1 release after LX-stimulated Mφ ingestion of apoptotic PMN showed an increase from 30 min to 18 h (data not shown). Interestingly, the peptides MHC bp and MMK-1 also evoked an increase in TGF-β1 production (Figure 1D). In contrast, LXA4, MHC bp, and MMK-1 treatment of Mφ did not elicit MCP-1 release after 30 min or 18 h Mφ-PMN coincubation (Figure 1E).

LX-Stimulated Phagocytosis: The Role of cAMP, PKC, PI 3-kinase, and MAP Kinase

The signal transduction pathways mediating Mφphagocytosis of apoptotic PMN are poorly understood. A role for cyclicAMP in this process has been demonstrated. Consistent with previous findings for LXA4 (16), LXB4-stimulated phagocytosis was inhibited by the cell-permeable analogue 8-Br-cAMP and mimicked by the PKA inhibitor Rp-cAMP (vehicle, 34.5 ± 5.35; LXB4, 64.5 ±7.5;* 8-Br-cAMP, 21.6 ± 3.2; 8-Br-cAMP + LXB4, 17.63 ± 2.7; Rp-cAMP, 50.0 ± 2.98;* Rp-cAMP +LXB4, 68.0 ± 5.3;* data are % phagocytosis ±SEM; n = 4 *P < 0.05 versus control).

Given the important role of PI 3-kinase, PKC and erk as modulators of Mφ cytoskeletal and/or adhesive events critical to phagocytic activity (1), we explored their role in LX-stimulated phagocytosis of apoptotic PMN. Previous exposure of Mφ to the PKC inhibitor GF 109203X blunted LX-triggered phagocytosis (Table 1). The PI 3-kinase inhibitor LY 294002 also reduced LX-stimulated phagocytosis. In contrast, the MAP kinase inhibitor PD 98059 was without effect on LX-stimulated phagocytosis (control, 11.5 ± 3.4; LXB4, 21.5 ± 5.3; PD, 11.3 ± 3.6; PD +LXB4, 22.1 ± 4.6; % phagocytosis ± SEM). Interestingly, the serine-threonine phosphatase inhibitor okadaic acid inhibited LX-stimulated phagocytosis, suggesting a modulatory role for phosphatases in the resolution process (Table 1).

View this table:

Table 1. The effect of PKC, PI 3-kinase, and phosphatase inhibition on LX- stimulated phagocytosisa

Enhancement of Mφ Phagocytosis of Apoptotic PMN In Vitro: A PSR-Independent Mechanism

In the present study, LXB4-stimulated phagocytosis, similar to the LXA4-induced response (16), was inhibited by mAbs against Mφ CD36 and αvβ3 integrins (control, 14.5 ± 3.25; LXB4, 28.6 ± 6.16;*anti-CD36 mAb, 13.93 ± 3.49; anti-CD36 mAb +LXB4, 14.60 ± 4.44; anti-CD51/61 mAb, 14.10 ±1.83; anti-CD51/61 mAb + LXB4, 12.58 ± 2.36; % phagocytosis ± SEM; n = 3;*P < 0.05 versus control). The Mφ PSR is an important molecule in Mφ recognition of apoptotic cells in many systems (23). Here we evaluated the role of the PSR in LXA4 and LXB4-stimulated phagocytosis using PSR antisera. PSR antisera (200 μg/ml) did not inhibit LX-stimulated phagocytosis (Figure 2A). Exposure of Mφ to β1,3-glucan renders Mφ-PMN interaction PSR-dependent (25). Here we report that treatment of Mφ with 25 μg/ml β1,3-glucan (48 h) abolished both LXA4 and LXB4-stimulated phagocytosis (Figure 2B). To verify that both the PSR antisera and β1,3-glucan were functional in our system and capable of eliciting previously reported effects (23,25,28,29), we measured TGF-β1 release after 18-h treatment with PSR antisera or β1,3-glucan; both agents induced significant TGF-β1 production (Figure 2C).

Figure

Figure 2. LX-stimulated phagocytosis is phosphatidylserine receptor–independent. (A) Phosphatidylserine receptor (PSR) antisera did not block LX-stimulated phagocytosis. Mφ were treated with PSR antisera (200 μg/ml) or isotype control Ab on ice for 30 min before treatment with LXA4 (1 nM for 15 mins at 37°C) and subsequent coincubation with apoptotic PMN. Data are mean % phagocytosis ± SEM (n = 3);*P < 0.05. (B) β1,3-glucan treatment abolishes sensitivity of Mφ to LX-stimulated phagocytosis. Mφ were treated with 25 μg/ml β1,3-glucan for 48 h before treatment with LXA4 and LXB4 (1 nM for 15 mins at 37°C) and coincubation with apoptotic PMN for 30 min. Data are expressed as mean phagocytosis ± SEM (n = 4);*P < 0.05 versus control. (C) PSR Ab and β1,3-glucan treatment of Mφ elicits TGF-β1 release. TGF-β1 release from Mφ treated with control, PSR Ab (200 μg/ml), isotype control Ab, and β1,3-glucan (25 μg/ml) for 18 h at 37°C was determined by ELISA. Data are expressed as mean cytokine production ±SD.

LX Reprograms BMDM Phenotype

BMDM can be programmed to develop distinct phenotypes in response to exposure to specific cytokines, including TGF-β, IFN-γ/TNF-α, and TNF-α(30). IFN-γ/TNF-αstimulate BMDM to adopt a cytotoxic phenotype characterized by sustained NO production and a diminished phagocytic capacity (30). Exposure of rat BMDM to TNF-α stimulates phagocytosis of apoptotic PMN and is not associated with NO release, characteristic of a reparative phenotype. Here we investigated whether LX could stimulate phagocytosis in such cytokine-primed BMDM. LXA4 and stable LX analogues significantly stimulated naïve rat BMDM to phagocytose apoptotic PMN (Figure 3A).

Figure

Figure 3. LX stimulate phagocytosis of bone marrow–derived Mφ (BMDM) under basal conditions and after cytokine programming. (A) BMDM were treated with LXA4 (1 nM for 24 h), 15(R/S)-methyl-LXA4, and 16-phenoxy-LXA4 (1 ×10−11 M for 24 h). BMDM were treated with (B) tumor necrosis factor–α (TNF-α) (10 ng/ml for 24 h) or (C) interferon-γ (IFN-γ)/TNF-α (10 ng/ml for 4 h and 10 ng/ml for 20 h) before treatment with LXA4 (1 nM for 15 mins), 15(R/S)-methyl-LXA4 or 16-phenoxy-LXA4 (1 ×10−11 M for 15 mins). After 30-min coincubations with aged PMN, phagocytosis was assessed by staining the cocultures for MPO activity. A minimum of 400 cells were counted to determine the number of Mφ that had ingested one or more PMN. Results are expressed as fold/basal phagocytosis ± SEM (n = 4);*P < 0.05 versus control.

After programming by cytokine-programmed cohorts (Figure 3, B and C): TNF-α treatment (10 ng/ml for 24 h) stimulated a significant increase in phagocytosis that was further enhanced by stimulation with LXA4 (1 ×10−9 M for 15 min), 15(R/S)-methyl-LXA4 (1 × 10−11 M for 15 min), or 16-phenoxy-LXA4 (1 ×10−11 M for 15 min) (Figure 3B). Exposure to IFN-γ/TNF-α (10 ng/ml for 4 h and 10 ng/ml for 24 h) reduced the phagocytic capacity of BMDM by approximately 25%, which is compatible with previous data (30). Intriguingly, subsequent exposure of such treated MDM to LXA4 (1 × 10−9 M for 15 min), 15(R/S)-methyl-LXA4 (1 × 10−11 M for 15 min), or 16-phenoxy-LXA4 (1 ×10−11 M for 15 min) rescued this phenotype and a significant increase in phagocytosis was observed comparable to naïve BMDM (Figure 3C).

LX Stimulate Mφ Phagocytosis of Exogenously Administered Apoptotic PMN In Vivo

To determine if LX stimulate Mφ phagocytosis of apoptotic PMN in vivo, we employed the mouse (BALB/C) thioglycollate-elicited peritonitis model (3). An enriched population of inflammatory Mφ were elicited with thioglycollate, and mice were then treated with a bolus intraperitoneal injection of LXA4 (1 or 2 μg for 15 min) before instillation of CMO-labeled apoptotic human PMN. It was necessary to use this approach given the difficulties in assessing endogenous generation and processing of apoptotic cells during acute inflammation. Thiry minutes after the instillation of apoptotic PMN intraperitoneally, lavaged cells were assayed by fluorescence microscopy. Figure 4A shows CMO-labeled apoptotic PMN, autofluorescence lavaged macrophages, and lavaged macrophages from LX-treated mice. LXA4 (1 μg and 2 μg) stimulated a 1.8-fold and a 2.2-fold increase in phagocytosis, respectively, as demonstrated in Figure 4B. Significant enhancement of phagocytosis was also seen in LXA4-treated mice when lavages were performed 15 min and 30 min after the instillation of apoptotic PMN (Figure 4C). The ability of LXB4, the 15 epimeric ATL, and stable synthetic LXA4 analogue to influence clearance of apoptotic PMN at an inflammatory site was also investigated. As seen with LXA4, LXB4 (1 μg) promoted phagocytosis in vivo an action shared with the aspirin-triggered epimer 15-epi-LXB4 (0.5 μg) and 15(R/S)-methyl-LXA4 (0.5 μg), which secured a twofold increase in phagocytosis (Figure 5). In aggregate, these data provide the first demonstration that native LX, ATL, and LX stable analogues promote clearance of apoptotic PMN in vivo and support their role as rapidly acting endogenous pro-resolution agents in inflammation.

Figure

Figure 4. LX rapidly stimulate Mφ phagocytosis of apoptotic PMN in vivo. BALB/C adult mice were treated with thioglycolate (3%) for 5 d before intraperitoneal administration of LX or vehicle (15 min) before instillation of aged human CMO-labeled PMN. Cytospin preparations of peritoneal lavages were prepared, and ingested PMN was quantified by fluorescent microscopy. In each experiment, a minimum of 400 cells was counted and the number of Mφ containing one or more apoptotic PMN was expressed as a percentage of the total number of Mφ. (A) Upper panel, CMO-labeled aged PMN;middle panel, auto-fluorescent lavaged macrophages; lower panel, LXA4-stimulated Mφ phagocytosis of apoptotic PMN in vivo; the thin arrows point to ingested PMN. (B) LXA4 stimulates phagocytosis in vivo. A 250-μl bolus of vehicle or LXA4 (1 μg and 2 μg) was administered intraperitoneally for 15 min before a 30-min PMN coincubation. Phagocytosis was assessed as described above. Data are mean % phagocytosis ± SEM (n = 3); *P < 0.005 versus control. (C) LXA4 significantly stimulates phagocytosis after 15-min and 30-min coincubation with excess apoptotic PMN. Mice were treated with 1 μg LXA4 for 15 min before either a 15-min or a 30-min coincubation with apoptotic PMN in vivo. Phagocytosis was assayed as described above. Data are expressed as mean % phagocytosis ± SEM (n = 3);*P < 0.005;**P < 0.0005 versus control.

Figure

Figure 5. LX-stimulated Mφphagocytosis of apoptotic PMN in vivo. An action shared with the ATL and the LX stable analogue. A 250-μl bolus of control, LXB4 (1 μg,), 15(R/S)-methyl-LXA4 (0.5 μg), and 15-epi-LXB4 (0.5 μg) was given intraperitoneally for 15 min before a 30-min PMN coincubation. Phagocytosis was determined as described in Figure 4. Data are expressed as mean % phagocytosis ± SEM (n = 3 to 5);*P < 0.05;***P < 0.0005 versus control.

Discussion

Here we demonstrate that LX (LXA4, LXB4, ATL, and stable synthetic LX analogues) and the peptide mimetics (MHC bp and MMK-1) can promote non-phlogistic phagocytosis of apoptotic PMN in vitro that is coupled to TGF-β1 release, insensitive to PSR antisera, and involves the CD36-αvβ3 complex. We demonstrate that LX stimulate phagocytosis of exogenously administered excess apoptotic PMN in a murine model in vivo, suggesting that LX rapidly promote the clearance of apoptotic leukocytes within an inflammatory milieu. Non-phlogistic clearance of apoptotic leukocytes by phagocytosis is an important determinant of the resolution of inflammation The benefits of such phagocytosis are not restricted merely to clearance of potentially lytic cells; such phagocytosis may be coupled to the active suppression of inflammation. Ultrastructural studies have demonstrated apoptotic uptake by Mφ in glomerulonephritis (31), whereas the persistence of apoptotic bodies in the glomeruli of C1q-deficient mice is indicative of impaired clearance (32).

To investigate the mechanism whereby LX-stimulated Mφphagocytosis of apoptotic PMN, we have used in vitro models: LX-stimulated human Mφ and cytokine-primed rat BMDM. In preliminary experiments, we investigated whether LX might stimulate phagocytosis of targets other than apoptotic PMN, namely latex beads and apoptotic lymphocytes. However, in our experiments the level of phagocytosis observed basally with such materials is extremely high, and it was not possible to observe an incremental increase in response to LX.

BMDM are essentially uncommitted phagocytes that can develop phenotypically distinct properties after cytokine programming, resulting in the development of inflammatory (TGF-β), cytotoxic (IFN-γ/TNF-α), and reparative (TNF-α) phenotypes (30). Exposure of TNF-α–programmed Mφ to LX further enhances their ability to phagocytose apoptotic PMN, whereas LX rescues the compromised phagocytic activity of TNF-α– and IFN-γ–primed Mφ. These data suggest that LX may stimulate PMN clearance by Mφ within the complex multi-mediator milieu that typifies inflammation in vivo.

To identify the signaling molecules involved in LX-stimulated phagocytosis, we have employed modulators of pathways implicated in LX signaling (15,33) and/or phagocytosis (reviewed in reference 34). We demonstrate a role for PKC and PI 3-kinase in LX-stimulated phagocytosis. It is likely that LXA4 and LXB4 stimulate phagocytosis through novel PKC isoforms (35) on the basis of the contrasting actions of the two LX on calcium mobilization (36). The 3′-phosphorylated substrates of PI 3-kinase are important regulators of several downstream components of phagocytosis, including Rho activation, a consequence of LX stimulation of Mφ(37).

A modulatory role for cAMP is suggested by our observations that both LXA4- (16) and LXB4-stimulated phagocytosis was inhibited by cell-permeable 8 Br-cAMP and mimicked by a PKA inhibitor. In this context, it is noteworthy that phosphorylation of CD36 by PKA may reduce its ability to bind apoptotic cells (16,38);furthermore, phagocytosis is dependent on cytoskeletal rearrangements, which are regulated by monomeric GTPases, including Rho (39), and Rho activation is negatively regulated by PKA (40).

The cell surface molecules involved in Mφ-apoptoticyte recognition include lectins, integrins, such as Mφαvβ3 that co-operate with CD36, PSR, scavenger receptors, and CD14 and specific bridging molecules, including thrombospondin and C1q (1). The ligand for Mφreceptors on apoptotic cells are less well documented; activation of a phospholipid scramblase in apoptotic cells results in exposure of phosphatidylserine (41), a ligand for the recently described Mφ PSR (23). PS-dependent phagocytosis of apoptotic leukocytes by mouse thioglycollate-elicited peritoneal Mφ has previously been shown, whereas quiescent Mφ were reported to utilize an αvβ3-dependent mechanism (1). Recent data indicate that phagocytosis by either activated or quiescent Mφ is PSR-dependent (23,29). Here we report that in monocyte-derived Mφ LX-stimulated phagocytosis required the αvβ3-CD36 complex but was independent of the PSR. This conclusion is based on sensitivity to anti-αvβ3 and CD36 antisera and insensitivity to PSR antisera. Further evidence of PSR independence are provided from β1,3-glucan–treated Mφ. This maneuver subverts the αvβ3-CD36 system to a PS-dependent mechanism (25). LX-dependent phagocytosis was not observed in β1,3-glucan–treated Mφ (importantly, the previously documented effects of stimulation of Mφ with PSR antisera and with β1,3-glucan on stimulating TGF-βrelease (25,28,42) were observed). Therefore, LX-stimulated phagocytosis of apoptotic cells is not necessarily dependent on the PSR. An analogous situation has recently been described by Cocco and Ucker (43) and for dexamethasone-augmented phagocytosis (44). A role for PS in LX-stimulated phagocytosis is not excluded as PS may bind to alternative receptors, e.g., a ligand for the Axl/Tyro3 Mer family of receptor tyrosine kinase growth arrest specific gene 6 (GAS 6) mediates PS binding to Mφ(45), and PS facilitates CD36-TSP-αvβ3–mediated uptake of apoptotic cells (25).

The LXA4 receptor (ALXR) (also known as the FPRL-1 receptor) is a G-protein–coupled receptor (46) expressed in diverse cell types (4). LXB4 does not compete with [3H]LXA4 for binding at the ALXR in PMN (47). In addition to binding LXA4 and its analogues, the ALXR has recently been shown to bind specific peptides (19,27), the acute phase protein serum amyloid A (SAA) (48), prion protein (49), and β amyloid (50). More recently, an additional receptor for peptide agonists has been described, namely FPRL-2. FPRL-2 is expressed on monocyte/macrophages but not PMN (51). LX and its analogues act via the ALXR to inhibit PMN chemotaxis. In contrast to the inhibitory effects of LX on PMN function, LX have been reported to activate monocyte chemotaxis, and this effect is shared with the ALXR peptide ligands (18). This contrasting data obtained from peptide and LX ligands in monocytes and PMN suggests involvement of the FPRL-2 receptor (19,27,51). It was of interest to investigate whether peptide agonists of ALXR could mimic the effects of LX on phagocytosis of apoptotic PMN. The peptides investigated were an MHC bp derived from mitochondrial NADPH dehydrogenase and a synthetic rogue peptide (MMK-1) (19,27). Exposure of Mφ to either peptide stimulated phagocytosis to levels comparable to those seen with LX. Interestingly, additivity was not observed on addition of suboptimal concentrations of LX and peptides together, suggesting a common mechanism. The release of N-formylated peptides from mitochondria of damaged cells is a signal for PMN chemotaxis. On the basis of our data, we propose that MHC bp and MMK-1 peptides might also act to stimulate macrophage phagocytosis of apoptotic cells via the ALXR or the more recently described FPRL-2 (51). We have verified the potential for peptide-activated phagocytic activity in the resolution of inflammation, showing that it is coupled to TGF-β1 release.

Our data demonstrating LX-stimulated phagocytosis of apoptotic PMN in vitro suggest a role for this process in promoting the resolution of inflammation in vivo. Consistent with this hypothesis are the data that demonstrate that LXA4 and LXB4 stimulated phagocytosis of human apoptotic PMN in the murine BALB/C model of thioglycollate-induced peritonitis, an established model of inflammation (3,52). Furthermore, the aspirin-triggered 15-epi-LXB4 and the stable LX analogue 15(R/S)-methyl-LXA4 (19) also stimulated phagocytosis. The enhanced efficacy and stability of LX analogues in modulation of leukocyte trafficking in models of inflammation and second organ injury after local and systemic administration has led to the proposal that these compounds might have therapeutic potential (17–20). Consistent with this are our data that highlight the attractiveness of LX stable analogues, which are resistant to metabolic inactivation by monocytes/macrophages yet retain the activity of the native LX (17,19,21). Comparable stimulation of phagocytosis was observed with 0.5-μg doses of 15(R/S)-methyl-LXA4 and 15-epi-LXB4 to that seen with 2-μg doses of the native LXA4. However, it was not feasible to investigate detailed dose-response relationships; therefore, conclusions about relative potency and efficacy in this model cannot be made. In previous reports (19), 10 μg of intravenously administered ATL were found to be effective in modulating leukocyte trafficking; it is obviously difficult to make a direct comparison between the availability via the different routes of administration. As described previously (see above), aspirin acetylation of COX-2 promotes the biosynthesis of epimeric LX (18), which can act locally to downregulate leukocyte activity (18–20) and, as suggested by our data, promote the clearance of apoptotic PMN from an inflammatory site. This may be particularly relevant in inflammation, where COX-2 expression is upregulated (4) and may have clinical implications for the use of COX-2 inhibitors, which leave the 15 lipoxygenase activity of the enzyme intact.

Acknowledgments

We are grateful to Valerie Fadok for providing PSR antisera. We thank Paola Maderna for helpful comments during the preparation of this manuscript. Work in the authors’ labs are supported by the Wellcome Trust (047273 [JS]; 058601 [HRB & CG]), the Health Research Board Ireland (CG, HRB), Enterprise Ireland (CG, HRB), the Punchestown Renal Research Fund (HRB), the National Kidney Research Fund (R22/1/99 [JS]), the EU (JS,CG,HRB), and the NIH (P01DE13499 [NP]).

References

  1. ↵
    Savill J, Fadok VA: Corpse clearance defines the meaning of cell death. Nature 407: 784–787, 2000
    OpenUrlCrossRefPubMed
  2. ↵
    Johnson R, Klebanoff SJ, Couser WG: Cellular mediators of immune injury: Neutrophils, In: Immunologic Renal Diseases,edited by Neilson EG, Couser WG: Philadelphia, Lippincott-Raven, 1997, pp 547–560
  3. ↵
    Taylor PR, Carugati A, Fadok VA, Cook HT, Andrews M, Carroll MC, Savill JS, Henson PM, Botto M, Walport MJ: A hierarchical role for classical pathway complement proteins in the clearance of apoptotic cells in vivo. J Exp Med 192: 3359–3366, 2000
    OpenUrl
  4. ↵
    McMahon B, Mitchell S, Brady HR, Godson C: Lipoxins: Revelations on resolution. TiPS 22: 391–395, 2001
  5. ↵
    Serhan CN, Hamberg M, Samuellson B: Lipoxins: Novel series of biologically active compounds formed from arachidonic acid in human leukocytes. Proc Natl Acad Sci USA 81: 5335–4339, 1984
    OpenUrlAbstract/FREE Full Text
  6. ↵
    Papayianni A, Serhan CN, Philips ML, Rennke HG, Brady HR: Transcellular biosynthesis of Lipoxin A4 during adhesion of platelets and neutrophils in experimental immune complex glomerulonephritis. Kidney Int 47: 1295–1302, 1994
    OpenUrl
  7. ↵
    Papayianni A Serhan, CN and Brady HR Lipoxin A4 and B4 inhibit leukotriene-stimulated interactions of human neutrophils and endothelial cells. J Immunol 156: 2264–72, 1996
    OpenUrlAbstract
  8. ↵
    Mayadas TN, Mendrick DL, Brady HR, Tang T, Papayianni A, Assmann K: Acute passive anti-glomerular basement membrane nephritis in P-selectin deficient mice. Kidney Int 49: 1342–1349, 1996
    OpenUrlCrossRefPubMed
  9. ↵
    Munger KA, Montero A, Fukunaga M, Uda S, Yura T, Imai E, Kaneda Y, Valdivielso JM, Badr KF: Transfection of rat kidney with human 15-lipoxygenase suppresses inflammation and preserves function in experimental glomerulonephritis. Proc Natl Acad Sci USA 96: 13375–13380, 1999
    OpenUrlAbstract/FREE Full Text
  10. ↵
    Katoh T, Lakkis FG, Makita N, Badr KF: Co-regulated expression of glomerular 12/15-lipoxygenase and interleukin-4 mRNAs in rat nephrotoxic nephritis. Kidney Int 46: 341–349, 1994
    OpenUrlCrossRefPubMed
  11. ↵
    Hachicha M, Pouliot M, Petasis NA, Serhan CN: Lipoxin [LX] A4 and aspirin-triggered 15-epi-LXA4 inhibit tumor necrosis factor alpha-initiated neutrophil responses and trafficking: regulators of a cytokine-chemokine axis. J Exp Med 189: 1923–1929, 1999
    OpenUrlAbstract/FREE Full Text
  12. ↵
    Gronert K, Gewirtz A, Madara JL, Serhan CN: Identification of a human enterocyte lipoxin A4 receptor that is regulated by interleukin[IL]-13 and interferon-γ and inhibits tumor necrosis factor α-induced IL-8 release. J Exp Med 187: 1285–1294, 1998
    OpenUrlAbstract/FREE Full Text
  13. ↵
    Sodin-Semrl S, Taddeo B, Tseng D, Varga J, Fiore S: Lipoxin A4 inhibits IL-1 β-induced IL-6, IL-8 and matrix metalloproteinase-3 production in human synovial fibroblasts and enhances synthesis of tissue inhibitors of metalloproteinases. J Immunol 164: 2660–2666, 2000
    OpenUrlAbstract/FREE Full Text
  14. ↵
    Bandeira-Melo C, Bozza PT, Diaz BL, Cordeiro RS, Jose PJ, Martins MA, Serhan CN: Cutting Edge: Lipoxin (LX) A4 and aspirin-triggered 15-epi-LXA4 block allergen induced eosinophil trafficking. J Immunol 164: 2267–2271, 2000
    OpenUrlAbstract/FREE Full Text
  15. ↵
    McMahon B, Stenson C, McPhilips F, Fanning A, Brady H, Godson C: Lipoxin A4 antagonizes the mitogenic effects of leukotriene D4 in human renal mesangial cells. J Biol Chem 275: 27566–27575, 2000
    OpenUrlAbstract/FREE Full Text
  16. ↵
    Godson C, Mitchell S, Harvey K, Petasis NA, Hogg N, Brady HR: Cutting edge: Lipoxins rapidly stimulate nonphlogistic phagocytosis of apoptotic neutrophils by monocyte-derived macrophages. J Immunol 164: 1663–1667, 2000
    OpenUrlAbstract/FREE Full Text
  17. ↵
    Maddox JF, Colgan SP, Clish CB, Petasis NA, Folkin VV, Serhan CN: LXB4 regulates human monocyte/neutrophil adherence and motility: design of stable LXB4 analoges with increased biological activity. FASEB J 12: 487–494, 1998
    OpenUrlPubMed
  18. ↵
    Claria J, Serhan CN: Aspirin triggers previously undescribed bioactive eicosanoids by human endothelial cell-leukocyte interactions. Proc Natl Acad Sci USA 92: 9475–9479, 1995
    OpenUrlAbstract/FREE Full Text
  19. ↵
    Chiang N, Fierro IM, Gronert K, Serhan CN: Activation of lipoxin A4 receptors by aspirin-triggered lipoxins and select peptides evokes ligand-specific responses in inflammation. J Exp Med 191: 1197–1207, 2000
    OpenUrlAbstract/FREE Full Text
  20. ↵
    Clish CB, O’Brien JA, Gronert K, Stahl GL, Petasis NA, Serhan CN: Local and systemic delivery of a stable-aspirin-triggered lipoxin prevents neutrophil recruitment in vivo. Proc Natl Acad Sci USA 96: 8247–8252, 1999
    OpenUrlAbstract/FREE Full Text
  21. ↵
    Serhan CN, Maddox JF, Petasis NA, Akritopoulou-ZanzeI, Papayianni A, Brady HR, Colgan SP, Madara JL: Design of Lipoxin A4 stable analogues that block transmigration and adhesion of human neutrophils. Biochemistry 34: 14609–14615, 1995
    OpenUrlCrossRefPubMed
  22. ↵
    Savill JS, Hogg N, Ren Y, Haslett C: Thrombospondin cooperates with CD36 and the vitronectin receptor in macrophage recognition of neutrophils undergoing apoptosis. J Clin Invest 90: 1513–15, 1992
  23. ↵
    Fadok VA, Bratton DL, Rose DM, Pearson A, Ezekwitz RAB, Henson PM: A receptor for phosphatidylserine specific clearance of apoptotic cells. Nature 405: 85–90, 2000
    OpenUrlCrossRefPubMed
  24. ↵
    Rossi AG, McCutcheon J, Roy N, Chilvers ER, Haslett C, Dransfield I: Regulation of macrophage phagocytosis of apoptotic cells by cAMP. J Immunol 160: 3562–3568, 1998
    OpenUrlAbstract/FREE Full Text
  25. ↵
    Fadok VA, Warner ML, Bratton DL, Henson PM: CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor [αv: β3]. J Immunol 161: 6250–6257, 1998
    OpenUrlAbstract/FREE Full Text
  26. ↵
    Lake FR, Nobel PW, Henson PM, Richies DW: Functional switching of macrophage responses to tumor necrosis factor-α (TNF-α) by IFNs: Implications for the pleiotropic activities of TNF-α. J Clin Invest 93: 1661–16, 1994
  27. ↵
    Klein C, Paul JI, Sauve K, Schmidt MM, Arcangeli L, Ransom J, Trueheart J, Manfredi JP, Broach JR, Murphy AJ: Identification of surrogate agonists for the human FPRL-1 receptor by autocrine selection in yeast. Nature Biotechnol 16: 1334–1337, 1998
    OpenUrlCrossRefPubMed
  28. ↵
    Fadok VA, Bratton DL, Konowal A, Freed PW, Westcott JY, Henson PM: Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGFβ, PGE2, and PAF. J Clin Invest 101: 890–898, 1997
    OpenUrl
  29. ↵
    Fadok V, deCathelineau A, Daleke DL, Henson PM, Bratton DL: Loss of phospholipid asymmetry and surface exposure of phosphatidylserine is required for phagocytosis of apoptotic cells by macrophages and fibroblasts. J Biol Chem 276: 1071–1077, 2001
    OpenUrlAbstract/FREE Full Text
  30. ↵
    Erwig LP, Kluth DC, Walshe GM, Rees AJ: Initial cytokine exposure determines function of macrophages and renders them unresponsive to other cytokines. J Immunol 161: 1983–1988, 1998
    OpenUrlAbstract/FREE Full Text
  31. ↵
    Savill J, Smith J, Sarraf C, Ren Y, Abbott F, Rees A: Glomerular mesangial cells and inflammatory macrophages ingest neutrophils undergoing apoptosis. Kidney Int 42: 924–936, 1992
    OpenUrlCrossRefPubMed
  32. ↵
    Botto M, Dell’Agnola C, Bygrave AE, Thompson EA, Cook HT, Petry F, Loos M, Pandolfi PP, Walport MJ: Homozygous C1q deficiency causes glomerulonephritis associated with multiple apoptotic bodies. Nature Genetics 19: 56–59, 1998
    OpenUrlCrossRefPubMed
  33. ↵
    Maderna P, Godson C, Hannify G, Murphy M, Brady HR: Influence of lipoxin A4 and other lipoxygenase-derived eicosanoids on tissue factor expression. Am J Physiol Cell 279: 945–953, 2000
    OpenUrl
  34. ↵
    Kwiatkowska K, Sobota A: Signalling pathways in phagocytosis. Bioessays 21: 422–431, 1999
    OpenUrlCrossRefPubMed
  35. ↵
    Larsen EC, DiGennaro J, Saito N, Mehta S, Loegering DJ, Mazurkiewicz JE, Lennartz MR: Differential requirement for classic and novel PKC isoforms in respiratory burst and phagocytosis in RAW 264.7 cells. J Immunol 165: 2809–2817, 2000
    OpenUrlAbstract/FREE Full Text
  36. ↵
    Romano M, Maddox J, Serhan CN: Activation of human monocytes and the acute monocytic leukemia cell line (THP-1) by lipoxins involves unique signaling pathways for LXA4 versus LXB4. J Immunol 157: 2149–2154, 1996
    OpenUrlAbstract
  37. ↵
    Maderna P Cottell, D. Berlasconi G Petasis, NA Brady HR, Godson C: Lipoxins induce actin rearrangement in monocytes and macrophages but not in neutrophils. Am J Pathol 160: 2275–2283, 2002
    OpenUrlCrossRefPubMed
  38. ↵
    Ren Y, Silverstein RL, Allen J, Savill JS: CD36 gene transfer confers capacity for phagocytosis of cells undergoing apoptosis. J Exp Med 181: 1857–1862, 1995
    OpenUrlAbstract/FREE Full Text
  39. ↵
    Hall A, Aderem A: Mechanisms of phagocytosis. Curr Opin Immunol 8: 36–40, 1996
    OpenUrlCrossRefPubMed
  40. ↵
    Lang P, Gerbert F, Delespine-Carmagnat M, Stancou R, Pouchelet M, Bertoglio J: Protein kinase A phosphorylation of Rho A mediates the morphological and functional effects of cAMP in cytotoxic lymphocytes. EMBO J 15: 510–519, 1996
    OpenUrlCrossRefPubMed
  41. ↵
    Frasch SC, Henson PM, Kailey JM, Janes DM, Fadox VA, Bratton DL: Regulation of phospholipid scramblase activity during apoptosis and cell activation by protein kinase C delta. J Biol Chem 275: 23065–23073, 2000
    OpenUrlAbstract/FREE Full Text
  42. ↵
    McDonald PP, Fadok VA, Bratton D, Henson PM: Transcriptional and translational regulation of inflammatory mediated production by endogenous TGF-β in macrophages that have ingested apoptotic cells. J Immunol 163: 6164–6171, 1999
    OpenUrlAbstract/FREE Full Text
  43. ↵
    Cocco RE, Ucker DS: Distinct modes of macrophages recognition for apoptotic and necrotic cells are not specified exclusively by phosphatidylserine exposure. Mol Biol Cell 12: 919–930, 2001
    OpenUrlAbstract/FREE Full Text
  44. ↵
    Giles KM, Ross K, Rossi AG, Hotchin NA, Haslett C, Dransfield I: Glucocorticoid augmentation of macrophage capacity for phagocytosis of apoptotic cells is associated with reduced p130Cas expression, loss of paxillin/pyk2 phosphorylation, and high levels of active Rac. J Immunol 167: 976–986, 2001
    OpenUrlAbstract/FREE Full Text
  45. ↵
    Scott R, McMahon EJ, Pop SM, Reap EA, Caricchio R, Cohen PL, Shelton Earp H, Matsushima GK: Phagocytosis and clearance of apoptotic cells is mediated by MER. Nature 411: 207–210, 2001
    OpenUrlCrossRefPubMed
  46. ↵
    Fiore S, Maddox J, Perez HD, Serhan CN: Identification of a human cDNA encoding a functional high affinity lipoxin A4 receptor. J Exp Med 180: 253–260, 1994
    OpenUrlAbstract/FREE Full Text
  47. ↵
    Fiore S, Ryeom SW, Weller PF, Serhan CN: Lipoxin recognition sites. Specific binding of labeled Lipoxin A4 with human neutrophils. J Biol Chem 267: 16168–16179, 1992
    OpenUrlAbstract/FREE Full Text
  48. ↵
    Su SB, Gong W, Gao JL, Shen W, Dunlop NM, Murphy PM, Oppenheim JJ, Wang JM: A seven transmembrane, G- protein coupled receptor, FPRL1 mediates the chemotactic activity of serum amyloid A for human phagocytic cells. J Exp Med 189: 395–402, 1999
    OpenUrlAbstract/FREE Full Text
  49. ↵
    Yingying L, Yazawa H, Gong W, Yu Z, Ferrans VJ, Murphy PM, MingJ: Cutting Edge: The neurotoxic prion peptide fragment PrP106–126 is a chemotactic agonist for the G protein-coupled receptor formyl peptide receptor-like 1. J Immunol 166: 1448–1451, 2001
    OpenUrlAbstract/FREE Full Text
  50. ↵
    Le Y, Gong W, Tiffany HL, Tumanov A, Nedospasov S, Shen W, Dunlop NM, Gao JL, Murphy PM, Oppenheim JJ, Wang JM: Amyloid (beta)42 activates a G-protein-coupled chemoattractant receptor, FPR-like-1. J Neurosci 2, RC123, 2001
    OpenUrl
  51. ↵
    Christophe T, Karlsonn A, Dugave C, Rabiet MJ, Boulay F, Dahlgren C: The synthetic peptide Trp-Lys-Try-Met-Val-Met-NH2 specifically activates PMN through FPRL1/LXA4R and is an agonist for the orphan monocyte-expressed chemoattractant receptor FPRL2. J Biol Chem 276: 21585–21593, 2001
    OpenUrlAbstract/FREE Full Text
  52. ↵
    Huynh, M-L, Fadok, VA Henson, P Phosphatidylserine-dependent ingestion of apoptotic cells promotes TGF-β1 secretion and the resolution of inflammation. J Clin Invest 109: 41–50, 2002
    OpenUrlCrossRefPubMed
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Lipoxins, Aspirin-Triggered Epi-Lipoxins, Lipoxin Stable Analogues, and the Resolution of Inflammation: Stimulation of Macrophage Phagocytosis of Apoptotic Neutrophils In Vivo
Siobhan Mitchell, Graham Thomas, Killeen Harvey, David Cottell, Keira Reville, Giovanni Berlasconi, Nicos A. Petasis, Lars Erwig, Andrew J. Rees, John Savill, Hugh R. Brady, Catherine Godson
JASN Oct 2002, 13 (10) 2497-2507; DOI: 10.1097/01.ASN.0000032417.73640.72
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