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Molecular Medicine, Genetics, and Development
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Inducible Podocyte-Specific Gene Expression in Transgenic Mice

Tetsuya Shigehara, Concepcion Zaragoza, Chagriya Kitiyakara, Hideko Takahashi, Huiyan Lu, Marcus Moeller, Lawrence B. Holzman and Jeffrey B. Kopp
JASN August 2003, 14 (8) 1998-2003; DOI: https://doi.org/10.1681/ASN.V1481998
Tetsuya Shigehara
*Kidney Disease Section, Metabolic Diseases Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland; and †Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan.
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Concepcion Zaragoza
*Kidney Disease Section, Metabolic Diseases Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland; and †Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan.
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Chagriya Kitiyakara
*Kidney Disease Section, Metabolic Diseases Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland; and †Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan.
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Hideko Takahashi
*Kidney Disease Section, Metabolic Diseases Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland; and †Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan.
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Huiyan Lu
*Kidney Disease Section, Metabolic Diseases Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland; and †Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan.
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Marcus Moeller
*Kidney Disease Section, Metabolic Diseases Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland; and †Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan.
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Lawrence B. Holzman
*Kidney Disease Section, Metabolic Diseases Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland; and †Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan.
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Jeffrey B. Kopp
*Kidney Disease Section, Metabolic Diseases Branch, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland; and †Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan.
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Abstract

ABSTRACT. The podocyte plays a key role in glomerular function and glomerular disease. To facilitate studies of podocyte function, we have developed a transgenic mouse model with inducible expression in the podocyte. The tetracycline-inducible transgenic system facilitates gene expression with restricted cellular distribution and tight temporal control. Recently, Bujard and colleagues have developed a functionally improved reverse tetracycline–controlled transcriptional activator (rtTA) with substantially lower background in the off state (the absence of tetracycline) and greater inducibility in the on state (the presence of tetracycline). We used the human podocin (NPHS2) gene promoter to control expression of the rtTA cassette and bred these mice with a reporter mouse line that contains the cytomegalovirus minimal promoter and tetO promoter elements together with LacZ, encoding β-galactosidase. Dual transgenic mice, bearing both podocin-rtTA and tetO-LacZ transgenes, had no detectable expression in kidney or other organs in the absence of tetracycline. Administration of tetracycline in the drinking water was associated with podocyte expression of β-galactosidase, in a fashion that was time dependent (maximal at 1 wk) and dose-dependent (maximal at 2 mg/ml). Podocyte expression was confirmed in two ways: histochemical staining for β-galactosidase and double-immunostaining using the podocyte marker WT-1 and β-galactosidase. This transgenic system should aid future investigations of podocyte function. E-mail: jbkopp@nih.gov

Received March 4, 2003. Accepted April 22, 2003.

  • © 2003 American Society of Nephrology
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Journal of the American Society of Nephrology: 14 (8)
Journal of the American Society of Nephrology
Vol. 14, Issue 8
1 Aug 2003
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Inducible Podocyte-Specific Gene Expression in Transgenic Mice
Tetsuya Shigehara, Concepcion Zaragoza, Chagriya Kitiyakara, Hideko Takahashi, Huiyan Lu, Marcus Moeller, Lawrence B. Holzman, Jeffrey B. Kopp
JASN Aug 2003, 14 (8) 1998-2003; DOI: 10.1681/ASN.V1481998

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Inducible Podocyte-Specific Gene Expression in Transgenic Mice
Tetsuya Shigehara, Concepcion Zaragoza, Chagriya Kitiyakara, Hideko Takahashi, Huiyan Lu, Marcus Moeller, Lawrence B. Holzman, Jeffrey B. Kopp
JASN Aug 2003, 14 (8) 1998-2003; DOI: 10.1681/ASN.V1481998
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More in this TOC Section

  • Use of the Tetracycline System for Inducible Protein Synthesis in the Kidney
  • Milder Presentation of Recessive Polycystic Kidney Disease Requires Presence of Amino Acid Substitution Mutations
Show more Molecular Medicine, Genetics, and Development

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