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Disease of the Month
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Hantavirus Infection

Walter Muranyi, Udo Bahr, Martin Zeier and Fokko J. van der Woude
JASN December 2005, 16 (12) 3669-3679; DOI: https://doi.org/10.1681/ASN.2005050561
Walter Muranyi
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Udo Bahr
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Martin Zeier
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Fokko J. van der Woude
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Hantavirus has attracted more and more attention as an emerging pathogenic virus in the past decades. It causes two distinct human diseases: Hemorrhagic fever with renal syndrome (HFRS) and human pulmonary syndrome (HPS).

Reports on clinical entities possibly caused by hantaviruses in China and England backdate into the first millennium and the Middle Age, respectively (1,2). However, it lasted until 1951 to 1953 during the Korean War before hantaviruses found global attention. More than 3000 United Nations and US soldiers experienced an acute febrile illness with acute renal failure and shock and a mortality rate of 7% close to a small river called Hantaan (3,4; G. Schreiner, personal communication, Washington, 1993). The causative agent, Hantaan virus (HTNV), was identified in 1978 by Lee et al. (5). Until now, 21 different hantavirus species have been described, and more than 30 genotypes are characterized and can be found all over the world (6,7).

Biology and Epidemiology

Morphology

Hantaviruses comprise one of five genera of the virus family Bunyaviridae (8). They replicate in the cytoplasm of host cells and are composed of a spherical lipid envelope; four viral proteins; and three single-stranded, negative-sensed RNA segments designated S (small), M (medium), and L (large) that are coding for the nucleocapsid protein (NP), the surface envelope glycoproteins G1 and G2, and the RNA-dependent RNA polymerase, respectively (Figure 1). Additional minor open reading frames are present in the genomes of hantaviruses, but to date, no corresponding proteins were identified. NP, the main structural protein, is complexed with the viral RNA genome segments that form helical nucleocapsids (9).

Figure 1.
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Figure 1.

Schematic of hantavirus morphology. The (-)ssRNA segments S (small), M (medium), and L (large) are encoded for the nucleocapsid protein, the glycoproteins G1 and G2, and the RNA-dependent RNA polymerase, respectively.

Host Range

The main natural reservoir of hantaviruses is murid rodents (order Rodentia; family Muridae; subfamilies Murinae, Arvicolinae, and Sigmodontinae). Virus and host share a long period of co-evolution characterized by the absence of any hantavirus-caused disease in infected rodents (10,11). Originally, it was thought that one rodent species is the predominant host for one hantavirus species, but recently more and more studies reveal that there might be multiple rodent hosts for individual virus species and multiple viruses in a single host species (12–14). In addition, numerous studies have reported hantavirus infections to be present in animal species other than rodents, for example, in cattle, moose, cat, dog, etc. However, the question of whether these animals are infected accidentally or represent further natural reservoirs has not yet been answered (15). The distribution of single hantavirus species correlates with the geographic extension of their hosts (Table 1), and hantavirus genotypes of the same geographic area are phylogenetically related (15–17).

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Table 1.

Main natural reservoirs and geographic distribution of pathogenic hantavirusesa

Humans do not belong to the natural host range of hantaviruses, and infection occurs accidentally via virus-containing, aerosolized rodent excretions such as urine, feces, or saliva. People who live or work in close contact with infected rodents are at increased infection risk, and studies usually show higher percentages of seropositive individuals in such groups as compared with control subjects (18,19).

Old World and New World Hantaviruses

The genus Hantavirus is roughly composed of two main groups: Old World and New World hantaviruses. HFRS in humans is caused by pathogenic Old World hantaviruses that include Amur virus, Seoul virus, and HTNV, the epidemiologically most important species, with lethality rates up to 15% in Asia, as well as Dobrava virus (DOBV), Tula virus (TULV), and Puumala virus (PUUV) in Europe; the last one is the main hantavirus species in Europe and induces Nephropathia epidemica (NE), a milder variant of HFRS, with mortality rates of 0.1% (10,20). HFRS affects approximately 200,000 people each year predominantly in Asia. In 2004, 235 cases were reported in Germany according to a recent epidemiologic bulletin of the Robert-Koch Institute.

The first pathogenic New World hantavirus (Sin Nombre virus) was discovered in the early 1990s in the Four Corners region of the United States (21). From this time on, numerous additional pathogenic New World hantaviruses were identified and characterized (Table 1). New World hantaviruses are the causative agent of approximately 300 cases of HPS each year in North and South America, with lethality rates up to 50%.

Human hantavirus infections are assumed to occur accidentally, and men represent a dead end for the hantavirus life cycle. Transfer of virus particles from infected to uninfected people normally does not occur. One exception is the Andes hantavirus strain Sout in Argentina, of which sporadical person-to-person transmissions were reported (22,23). This finding reveals a worrying risk potential of hantaviruses for human health.

Clinical Course of NE, HFRS, and HPS

HFRS and HPS are partly overlapping clinical syndromes. In Europe, the hantavirus serotype Puumala causes NE, a milder variant of HFRS. Viremia occurs after initial infection of alveolar macrophages and life-threatening acute-phase symptoms are induced primarily by infection of vascular endothelial cells of the lung and the kidneys with concomitant loss of barrier function resulting in severely increased endothelial permeability.

NE

NE is characterized by a sudden onset with high fever, headache, backache, and abdominal pain. Transient thrombocytopenia is a typical finding in the early phase of the disease. The occurrence of conjunctival hemorrhages, palatine petechiae, and a truncal petechial rash after 3 or 4 d is possible. Approximately 1% of patients experience severe neurologic manifestations, e.g., seizures or bladder paralysis. The hemorrhages are accompanied by oliguria, azotemia, proteinuria, and hematuria. Within 3 d, the rash disappears and the patients develop polyuria. The convalescent phase extends over several weeks, and sequelae are rare. Severe courses of NE with acute renal failure and lethal outcome range between 0.1 and 1% (24).

HFRS

The incubation period of HFRS is 7 to 36 d. Only 10 to 15% of cases have a severe course, with lethality rates between 6 to 15%. HFRS is characterized by systemic involvement of capillaries and venules. It induces various hemorrhagic manifestations and circulation disorders. Renal involvement is characterized by acute renal failure as a result of interstitial hemorrhage and interstitial infiltrates (24,25).

The clinical course is subdivided into five phases: Febrile, hypotensive, oliguric, diuretic, and convalescent. The onset of HFRS resembles NE with high fever, backache, abdominal pain, chills, myalgia, malaise, and bradycardia over 3 to 4 d. Photophobia, pharynx enanthema, and a diffuse reddening of the face are also observed. On the third to fifth days, petechia develop initially on the palate. At the same time, conjunctival hemorrhages may appear and a temporary impairment of the visual function is reported. The urinary sediment reveals hematuria and atypical gross proteinuria (in some cases >3 g/24 h). The hypotensive phase ranges 3 to 6 d after onset of fever. Shock or hypotension may occur. Laboratory findings in this phase are leukocytosis and thrombocytopenia. Patients show a wide range of renal conditions, including acute tubulointerstitial nephritis, necrotizing glomerulonephritis, and IgA nephropathy. The oliguric phase starts at approximately day 8, and hemorrhagic manifestations become more prominent. The diuretic phase starts at approximately day 11, and the convalescent phase lasts approximately 3 wk to 6 mo.

Sequelae are rare but include chronic renal failure and hypertension. In a series of 46 patients in Tampere (Finland) who had NE 3 to 7 y ago, the patients had higher GFR and filtration fraction, more proteinuria, and a higher ambulatory systolic BP compared with healthy control subjects (26). Furthermore, we studied 42 patients after NE from several areas in Germany and found hypertension or elevated serum creatinine (1.5 mg/dl) (27).

Extrarenal manifestations include acute impairment of visual function, acute myopia, CNS complications with seizures, sometimes myocarditis, and severe gastrointestinal hemorrhages. In addition, thyroid, liver, and pancreas may be affected. Lung involvement but to a lesser extent than in HPS is also observed during HFRS (24,28–30).

HPS

The onset of HPS is characterized by flu-like symptoms such as high fever, myalgia, and headache. The patients develop acute noncardiac pulmonary edema and hypotension within 2 to 15 d. Bilateral infiltrates develop rapidly, sometimes associated with pleural effusions. Neutrophilic leukocytosis, hemoconcentration, thrombocytopenia, and circulating immunoblasts are observed. Severe courses of HPS are associated with increased lactate levels. The mortality rates of HPS are approximately 50%. Patients who survive the acute phase of the disease recover normally within 5 to 7 d without any sequelae (24). Acute renal failure is secondary as a result of shock and respiratory failure.

The medical knowledge about HFRS and HPS has increased substantially in the past years, resulting in the conclusion that both syndromes are partly overlapping. The number of reports on HFRS cases with lung involvement and HPS cases with renal involvement is continuously growing, and it is conceivable that the descriptions of the clinical courses of both syndromes will further converge in the near future.

Pathology of NE, HFRS, and HPS

NE and HFRS

Immunohistochemistry analysis of hantavirus-infected renal tissue reveals interstitial infiltrates with immune cells and interstitial hemorrhage. The most common histopathologic lesion are acute tubulointerstitial nephritis. Tubular epithelial and luminal alterations are present. Intertubular capillaries are congested, and the interstitium is broadened by edema, indicative of a generalized capillary damage. Occasionally, glomerular pathology, e.g., hypercellularity and expansion of the mesangium, are observed, and this is probably the underlying cause of gross proteinuria. Tubular, interstitial, and glomerular histologic damage are associated with the clinical severity of renal failure (Figure 2). It is of note that urinary sediment contains tubular cells with extremely enlarged nucleoli. These cells resemble uroepithelial tumor cells and spontaneously disappear after the disease has subsided (29,31). Recent work has shown that these tubular cells contain hantavirus antigen (32).

Figure 2.
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Figure 2.

(A) Focal mononuclear interstitial infiltration, capillary congestion, and interstitial hemorrhage at the corticomedullary junction. (B) Normal glomeruli. Focal interstitial edema with a mild mononuclear infiltrate and prominent endothelial cells of peritubular capillaries. Magnification, ×100 in A, Masson trichrome stain; ×160 in B, PAS stain.

HPS

Immunohistochemistry analysis of HPS documents the distribution of viral antigens within the endothelium of capillaries throughout various tissues. Infected endothelial cells lack any morphologic changes and show no visible cytopathic effects (CPE). Accumulations of hantaviral antigens are observed in the pulmonary microvasculature and in dendritic cells within the lymphoid follicles of spleen and lymph nodes. In some autopsy cases, endothelial cells in the capillaries of the myocardium and the endocardium bear hantavirus antigen, contributing substantially to severe courses of HPS.

Gross pathologic findings show that the lungs of patients with HPS are dense, rubbery, and heavy, usually weighing twice as much as the average lung. The pathologic lesions are primarily vascular with variable degrees of generalized capillary dilation and edema. Frequently, the lungs reveal a mild to moderate interstitial pneumonitis with variable degrees of congestion, edema, and mononuclear cell infiltration (33).

Pathophysiology of Hantavirus Infection

The main factor that determines the course and the severity of HFRS and HPS is the degree of increased permeability of infected endothelium that shows no histologic signs of damage and no visible CPE. At present, it is poorly understood how pathogenic hantaviruses induce the capillary leakage during the acute phase of the two syndromes and why some hantavirus species are nonpathogenic.

Genetic Predisposition

Patients with certain HLA antigens seem to have a genetic predisposition for severe courses of HFRS and HPS. Patients who bear HLA-B8, DRB1*0301, C4A*Q0, or DQ2 alleles seem to have a significantly higher risk for a severe course of NE (34–36), and the HLA-B35 allele was associated with severe courses of HPS (37). The mechanisms that are involved in these genetic predispositions are unknown.

Hantavirus Replication Cycle

Hantavirus replication takes place in macrophages and vascular endothelial cells, especially in the lung and the kidney (10,38). For pathogenic hantaviruses, the entry into host cells occurs by attachment to αVβ3 integrin on the cellular surface and subsequent endocytosis (39,40). The virion envelope fuses with the endosome membrane in a pH-dependent way, and nucleocapsids are released into the cytoplasm. Thereafter, the viral RNA-dependent RNA polymerase directs transcription of viral genes and replication of the viral RNA genome segments. The viral NP and RNA polymerase mRNA are translated at free ribosomes, whereas the glycoprotein mRNA is translated into the endoplasmic reticulum. G1 and G2 glycoproteins are transported to the Golgi complex for final glycosylation. Large intracellular inclusion bodies, probably composed of NP, are formed in the cytoplasm. It is assumed that hantavirions are formed at the membranes of the Golgi complex, followed by budding into the Golgi cisternae, migration in secretory vesicles to the plasma membrane, and release by exocytosis. Several in vitro studies have shown that this hantaviral life cycle does not induce any visible CPE in endothelial cells. Host cells are not lysed by infection with pathogenic hantaviruses, and no increased permeability is induced in endothelial cell cultures (41,42). Apoptosis and expression of apoptosis-related genes in cells that were infected with pathogenic hantaviruses was reported for cultured VeroE6 and human embryonic kidney cells; however, in vivo, there is no evidence for programmed cell death in infected endothelial cells (43–46). These data indicate that increased endothelial permeability during HFRS and HPS might be the result of the infection with pathogenic hantaviruses in combination with additional factors that are specific for the in vivo situation and that are not present in in vitro cell cultures. In this context, it is assumed that antiviral processes in the infected cells and immune mechanisms may play a key role in the development of vascular dysfunction (10,47).

Innate Antiviral Immune Responses

Infection with hantaviruses induces innate immune responses in host cells, whereas pathogenic hantaviruses seem to be able to evade these responses to a certain degree. Different types of interferons are expressed, and IFN-inducible genes are activated. The expression of the IFN-inducible MxA protein is delayed in cells that are infected with pathogenic hantaviruses in comparison with nonpathogenic hantaviruses. Similarly, levels of antigen-presenting molecules, e.g., HLA class I, are elevated; however, the upregulation proceeds more slowly after infection with pathogenic than with nonpathogenic hantaviruses (48–51). Other innate antiviral mechanisms that are induced during hantavirus infection include activation of the complement system of the classical and alternative route, e.g., with elevated titers of soluble terminal complex SC5b-9 and higher C4d/C4 ratios during NE caused by PUUV (52). Natural killer cells, known as effector and regulatory cells in innate and adaptive immunity in terms of production of cytotoxic molecules and secretion of cytokines and chemokines (10), are assumed to migrate into hantavirus-infected tissues (53,54).

Humoral Immune Response

The adaptive immune system counters a hantavirus infection via a humoral and a cellular response. In the course of the humoral immune response, all types of Ig are expressed during HFRS and HPS. Elevated titers of total serum and virus-specific IgA, the main immunologic component of the mucosa, were detected during the acute phase of both syndromes (55,56). Total and virus-specific IgE titers were found to be increased before and during the acute phase of HFRS. It is conceivable that IgE participates in hantavirus pathophysiology by activation of IL-1β and TNF-α secretion that could influence permeability of infected endothelium; however, it was not possible to find a correlation between IgE levels and HFRS severity (10,57,58). High titers of virus-specific IgM against viral NP, G1, and G2 are produced during and after the acute phase of HFRS and HPS, whereas the hantavirus NP is regarded as the major viral antigen (10,55,56,59,60). Virus-specific IgG, the most abundant antibody of total Ig against hantaviruses, is also predominantly directed against viral NP and appears during the acute phase of HFRS and HPS, whereas further increasing titers can be observed during the early convalescent phase (10,56,61).

Cellular Immune Response

Cytotoxic CD8+ T cells (CTL) are the predominant lymphocytes in the course of the cellular immune response to a hantavirus infection and are assumed to play important roles in virus clearing and HFRS/HPS pathogenicity. Increased numbers of CTL were observed at the onset of HFRS and HPS and were also found in the lungs of patients who died from HPS. The severity of disease generally correlates with the number of CTL (10,37,51). CTL epitopes were identified in all three viral structural proteins, whereas NP again seems to be the predominant immunogenic protein (62,63).

Secretion of Cytokines and Chemokines

Various types of chemokines and cytokines are secreted in variable amounts to regulate the immune response during a hantavirus infection. It is assumed that cytokines/chemokines play an important role in vascular dysfunction during HFRS and HPS. Many cytokines/chemokines, such as TNF-α, are known to increase endothelial permeability in the course of natural immune response mechanisms, e.g., during lymphocyte migration through the vascular walls. Significantly elevated plasma levels of IFN-γ, TNF-α, IL-2, and IL-6 were detected at the onset of the acute phase of HFRS and HPS (51,64,65). Increased titers of TNF-α seem to correlate with a severe course of NE (66). Increased expression of cytokines, especially of TNF-α in the peritubular area of the distal nephron, was reported during HFRS (67,68), and in the lungs of patients with HPS, increased numbers of IFN-γ–, IL-1α–, IL-1β–, IL-2–, IL-4–, IL-6–, and TNF-α/β–producing cells were observed (37). Hantaviruses are also able to infect dendritic cells, resulting in secretion of proinflammatory cytokines, e.g., IFN-α and TNF-α that could also contribute to increased endothelial permeability (65,69). In vitro infection of human lung microvascular endothelial cells with HTNV or Sin Nombre virus generated increased amounts of RANTES and 10-kD IFN-inducible protein (42). A recent in vitro study by Niikura et al. (70) showed that TNF-α–induced increased permeability of endothelial cells is significantly prolonged in HTNV-infected cells in comparison with uninfected cells.

Cellular Target Proteins

The cumulative data about hantavirus pathophysiology so far indicate that a hantavirus infection interferes in a thus far unknown way with vascular permeability regulation during inflammation, resulting in endothelial dysfunction. It is conceivable that this interference is mediated by interactions between viral and cellular proteins that participate in permeability regulation. Several studies identified associations of hantavirus NP with small ubiquitin-like modifier-1, with small ubiquitin-like modifier-1–interacting proteins and with the Fas-mediated apoptosis enhancer Daxx (71–74); however, none of these proteins is involved in permeability regulation in endothelial cells.

A much more promising candidate is αVβ3-integrin, the cellular surface receptor for pathogenic hantaviruses. αVβ3 Integrin participates in the regulation of cell-to-cell adhesion, platelet aggregation, and maintenance of vascular barrier function. The binding of hantaviruses to αVβ3 integrin inhibits β3 integrin–directed endothelial cell migration. Furthermore, it was shown that hantaviruses bind to so-called plexin-semaphorin-integrin domains that are present on the surface of inactive αVβ3 integrin molecules. These interactions are assumed to inhibit regular αVβ3 integrin functions and probably interfere with endothelial permeability regulation (39,40,74–76). A further study identified immunoreceptor tyrosine-based activation motifs within the G1 cytoplasmic tail of all HPS-causing hantaviruses. These G1 immunoreceptor tyrosine-based activation motifs bind key cellular kinases that regulate immune and endothelial cell functions. The implications of these interactions are not clear, but an influence on permeability regulation is possible (77).

Laboratory Diagnosis of Hantavirus Infections

Diagnosis of hantaviruses is usually made on the basis of clinical and serologic findings. Hantavirus should be performed in a patient with fever, lumbago, renal failure, and recent outdoor activities. In the early course of the disease, thrombocytopenia is detectable. An ELISA-based detection of NP-specific IgM antibodies is usually performed for laboratory diagnosis of an acute hantavirus infection (78). The highest titers are demonstrable between 8 and 25 d after onset of disease. It is important to note for the differential diagnosis of Puumala and Hantaan virus infections that PUUV NP-specific ELISA cross-reacts with HTNV NP, whereas HTNV NP-specific ELISA shows virtually no cross-reaction with PUUV NP (29). In addition, immunochromatographic assays (79) and reverse transcriptase–PCR have been used increasingly in recent years, but they have not yet become widely accepted as standard clinical laboratory tests (80,81).

Therapy for Hantavirus Infections

At present, there are no antiviral drugs that are applicable to cure hantavirus infections. The treatment of patients with HFRS or HPS is restricted to supportive procedures to keep under control the symptoms, which may be life-threatening. Patients are normally supervised in an emergency department or intensive care unit for close monitoring and care until the patient’s immune system has cleared the virus and the convalescent phase begins.

Ribavirin (1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide), a guanosine-analog, was shown to possess anti-hantaviral activity. Controlled trials in the early 1990s reported on decreased virus titers, higher survival rates, and decreased morbidity both in murine models and in patients with HFRS (82,83). However, newer ribavirin trials in patients with HPS did not confirm the promising results (10,84,85). Recently, it was shown that ribavirin inhibits the production of hantavirus progeny in vitro. The antiviral activity was due to incorporation of ribavirin into nascent RNA, resulting in high mutation frequencies (9.5/1000 nucleotides) and, hence, in the synthesis of transcriptionally defect viral RNA (86). The study showed that hantavirus RNA-dependent RNA polymerase is susceptible to drugs that lead to error catastrophes during the viral replication cycle. These insights allow new strategies for the development of therapeutic procedures that include the incorporation of lethal mutations during hantavirus replication (87).

Normally, a viral infection induces specific antiviral processes in target cells; among them is the expression of interferons and IFN-inducible genes. In VeroE6 cells, it was shown that pretreatment with human IFN-α, -β, and -γ leads to an inhibition of HTNV, PUUV, and TULV replication (88). The IFN-inducible human MxA protein, an intracytoplasmic GTPase of the dynamin superfamily, shows antiviral activity against a wide range of RNA viruses, including hantaviruses. Viral replication induces the expression of the MxA protein that was shown to interfere with the replication cycle of hantaviruses (46,89,90). However, up to now, no study has indicated how interferons and IFN-inducible proteins could serve as therapeutic agents during hantavirus infections.

Recently, Klingstrom et al. (91) passively immunized cynomolgus macaques with neutralizing mAb and subsequently challenged them with wild-type PUUV. A delayed onset of viremia and seroconversion was observed, and one of the immunized monkeys showed neither symptoms nor elevated levels of IL-6, IL-10, and TNF-α. The efficiency of passive immunization was also confirmed in an earlier study in a Syrian hamster model for lethal HPS using antibodies against Andes virus glycoproteins that were induced by DNA vaccines (92). Future clinical trials have to show whether passive immunization could represent a therapeutic instrument for the treatment of acute HFRS or HPS in humans.

In a recent Chinese study, it was reported that intracellularly applied single-chain Fv of mAb against HTNV NP was able to bind to the hantavirus nucleocapsid protein in the cytoplasm of infected cells. The method could represent a new therapeutic approach in the future (93).

Unusual approaches such as the therapeutic assessment of plant compounds directed against phleboviruses (plant viruses of the family Bunyaviridae) (94) or the application of integrated traditional Chinese medicine (95) were pursued to identify a treatment for hantavirus infections; however, up to now, none of these studies has provided a crucial breakthrough in HFRS or HPS therapy. It is conceivable that the elucidation of the molecular mechanisms of hantavirus pathophysiology or, more precise, the clarification of cellular processes that participate in endothelial dysfunction during HFRS and HPS in the future will provide new targets for effective therapeutic strategies.

Prevention of Hantavirus Infections

Because infection with some hantavirus species results in high morbidity and mortality rates and in view of the present situation of missing effective antiviral drugs, it is of particular importance to try to prevent an infection. On the one hand, it is indicated to avoid places where murid rodents live in large quantities to avert contact with virus-containing rodent excretions. This includes keeping homes and the near surrounding area rodent-free, for example, by eliminating crawl spaces and debris and removing food sources to make homes and work areas unattractive for rodents. On the other hand, many research efforts have been made in the past years to develop an effective and safe vaccine against hantaviruses applying vaccination techniques varying from killed virus to recombinant DNA technology. In Korea, a formalin-inactivated HTNV vaccine, Hantavax (Korea Green Cross, Seoul, Korea), that is produced from rodent brain–derived virus, is commercially available. Hantavax was shown to induce high titers of IgG-specific antibodies in almost 100% of human volunteers after three vaccinations accompanied by the production of neutralizing antibodies in approximately 80% of test individuals; however, the antibody titers declined very rapidly within months, and boosters yielded no satisfactory protection rates (96–99). Further studies confirmed that Hantavax elicited only protection rates between 30 and 50% for longer time periods (100). In another study, a VeroE6 cell culture–derived, formaldehyde-inactivated HTNV vaccine showed significantly higher antibody titer and protection rates in Balb/c mice in comparison with Hantavax; however, protection rates in humans were also very low (101). To this day, there are no hantavirus vaccines that are based on inactivated viruses and that elicit satisfactory protection rates in humans (10,98,102).

In addition to inactivated whole-virus particles, single viral components (the viral structural proteins NP, G1, and G2) were obtained with recombinant DNA technology, expressed in several cell culture systems and organisms, and tested for their immunogenicity and protective potential. For example, recombinant PUUV NP expressed in yeast induced protective immunity in bank voles (103), and recombinant NP of DOBV expressed in yeast induced high antibody titers in Balb/c and C57BL/6 mice (104). PUUV NP was expressed successfully in transgenic tobacco and potato plants by our group but failed to induce an antibody response in mice when administered as an oral vaccine (105,106). Recently, recombinant NP of DOBV was tested in combination with various adjuvants for immunogenicity and protective efficacy in C57/BL6 mice. The study identified Freund’s adjuvant as the additive of choice because mice that were vaccinated with this adjuvant in combination with the DOBV NP showed a protection rate from challenge of 75%, whereas the usage of other adjuvants such as Alum, which induces strong Th2-type immune responses, did not result in protective immunity (107).

Furthermore, known immunogenic epitopes of PUUV, DOBV, and HTNV NP were incorporated into chimeric hepatitis B virus core particles and elicited high antibody titers and protective immunity in bank voles (108,109). In addition, life recombinant viruses that express and carry hantavirus structural proteins were constructed. For example, HTNV NP, G1, and G2 expressed with baculovirus and vaccinia virus vectors were shown to induce protection after a Hantaan virus challenge in hamster and mouse models (110–112). A vaccinia-vectored Hantaan virus vaccine was tested in a Phase II, double-blinded, placebo-controlled clinical trial among 142 volunteers. Neutralizing antibodies to Hantaan virus were detected in 72% of the test individuals (113).

Finally, plasmid-based DNA vaccines, which express hantavirus structural proteins, were tested for their immunogenic and protective potential. Many groups introduced the coding sequences of the structural proteins of various pathogenic hantaviruses into usually CMV-based eukaryotic expression vectors and tested the immunogenic potential of these DNA vaccines in mouse, hamster, and Rhesus macaque models. The DNA vaccines always induced high antibody titers often of the neutralizing type (10,92,114–117). Despite the extensive work of many research groups on the field of hantavirus vaccine development and the presence of promising data in animal models, there is still no worldwide approved and commercially available vaccine against hantaviruses, and it seems unlikely that this situation will change in the near future.

Conclusion

Since the discovery of HTNV as the causative agent of HFRS, much knowledge about the various hantaviruses and their manifestations in animals and humans has been gathered. NE, HFRS, and HPS are human diseases, caused by hantaviruses, which may be encountered by clinical nephrologists. The diagnosis rests on serologic evidence. Supportive therapy is dependent on the Hantavirus strain and clinical symptoms, especially important in HFRS and HPS, for which correction of bleeding, maintenance of BP, and treatment of renal or respiratory insufficiency may be indicated. It is hoped that a better understanding of viral biology and pathophysiology will lead to more effective and specific therapeutic modalities in the future.

Acknowledgments

We are deeply grateful to Prof. Konrad Andrassy and Prof. Gholamreza Darai for generous support and helpful critical comments.

Footnotes

  • Published online ahead of print. Publication date available at www.jasn.org.

  • © 2005 American Society of Nephrology

References

  1. ↵
    Bridson E: The English “sweate” (Sudor Anglicus) and Hantavirus pulmonary syndrome. Br J Biomed Sci 58: 1–6, 2001
    OpenUrlPubMed
  2. ↵
    McCaughey C, Hart CA: Hantaviruses. J Med Microbiol 49: 587–599, 2000
    OpenUrlPubMed
  3. ↵
    Johnson KM: Hantaviruses: History and overview. Curr Top Microbiol Immunol 256: 1–14, 2001
    OpenUrlCrossRefPubMed
  4. ↵
    Smadel JE: Epidemic hemorrhagic fever. Am J Public Health 43: 1327–1330, 1953
  5. ↵
    Lee HW, Lee PW, Johnson KM: Isolation of the etiologic agent of Korean hemorrhagic fever. J Infect Dis 137: 298–308, 1978
    OpenUrlCrossRefPubMed
  6. ↵
    Tidona CA, Darai G: The Springer Index of Viruses, Springer-Verlag, Berlin, 2002
  7. ↵
    van Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB: Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses, Academic Press, San Diego, 2000
  8. ↵
    Schmaljohn CS, Dalrymple JM: Analysis of Hantaan virus RNA: Evidence of a new genus of Bunyaviridae. Virology 131: 482–491, 1983
    OpenUrlCrossRefPubMed
  9. ↵
    Plyusnin A, Vapalahti O, Vaheri A: Hantaviruses: Genome structure, expression and evolution. J Gen Virol 77: 2677–2687, 1996
    OpenUrlCrossRefPubMed
  10. ↵
    Maes P, Clement J, Gavrilovskaya I, Van Ranst M: Hantaviruses: Immunology, treatment, and prevention. Viral Immunol 17: 481–497, 2004
    OpenUrlCrossRefPubMed
  11. ↵
    Ulrich R, Hjelle B, Pitra C, Kruger DH: Emerging viruses: The case ‘hantavirus.’ Intervirology 45: 318–327, 2002
    OpenUrlCrossRefPubMed
  12. ↵
    Nemirov K, Henttonen H, Vaheri A, Plyusnin A: Phylogenetic evidence for host switching in the evolution of hantaviruses carried by Apodemus mice. Virus Res 90: 207–215, 2002
    OpenUrlCrossRefPubMed
  13. Scharninghausen JJ, Faulde M, Cavaljuga S: Hantavirus host/virus interactions within Southeast Europe. Bosn J Basic Med Sci 4: 13–18, 2004
    OpenUrlPubMed
  14. ↵
    Weidmann M, Schmidt P, Vackova M, Krivanec K, Munclinger P, Hufert FT: Identification of genetic evidence for Dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR and TaqMan RT-PCR. J Clin Microbiol 43: 808–812, 2005
    OpenUrlAbstract/FREE Full Text
  15. ↵
    Zeier M, Handermann M, Bahr U, Rensch B, Müller S, Kehm R, Muranyi W, Darai G: New ecological aspects of Hantavirus infection: A change of a paradigm and a challenge of prevention—A review. Virus Genes 30: 157–180, 2005
    OpenUrlCrossRefPubMed
  16. Plyusnin A, Morzunov SP: Virus evolution and genetic diversity of hantaviruses and their rodent hosts. Curr Top Microbiol Immunol 256: 47–75, 2001
    OpenUrlPubMed
  17. ↵
    Plyusnin A: Genetics of hantaviruses: Implications to taxonomy. Arch Virol 147: 665–682, 2002
    OpenUrlCrossRefPubMed
  18. ↵
    Deutz A, Fuchs K, Schuller W, Nowotny N, Auer H, Aspock H, Stunzner D, Kerbl U, Klement C, Kofer J: Seroepidemiological studies of zoonotic infections in hunters in southeastern Austria—Prevalences, risk factors, and preventive methods. Berl Munch Tierarztl Wochenschr 116: 306–311, 2003
    OpenUrlPubMed
  19. ↵
    Zoller L, Faulde M, Meisel H, Ruh B, Kimmig P, Schelling U, Zeier M, Kulzer P, Becker C, Roggendorf M, et al.: Seroprevalence of hantavirus antibodies in Germany as determined by a new recombinant enzyme immunoassay. Eur J Clin Microbiol Infect Dis 14: 305–313, 1995
    OpenUrlCrossRefPubMed
  20. ↵
    Vapalahti O, Mustonen J, Lundkvist A, Henttonen H, Plyusnin A, Vaheri A: Hantavirus infections in Europe. Lancet Infect Dis 3: 653–661, 2003
    OpenUrlCrossRefPubMed
  21. ↵
    Hjelle B, Jenison S, Torrez-Martinez N, Yamada T, Nolte K, Zumwalt R, MacInnes K, Myers G: A novel hantavirus associated with an outbreak of fatal respiratory disease in the southwestern United States: Evolutionary relationships to known hantaviruses. J Virol 68: 592–596, 1994
    OpenUrlAbstract/FREE Full Text
  22. ↵
    Padula PJ, Edelstein A, Miguel SD, Lopez NM, Rossi CM, Rabinovich RD: Hantavirus pulmonary syndrome outbreak in Argentina: Molecular evidence for person-to-person transmission of Andes virus. Virology 241: 323–330, 1998
    OpenUrlCrossRefPubMed
  23. ↵
    Pinna DM, Martinez VP, Bellomo CM, Lopez C, Padula P: New epidemiologic and molecular evidence of person to person transmission of hantavirus Andes Sout. Medicina (B Aires) 64: 43–46, 2004
  24. ↵
    Beers MH, Berkow R: Infectious diseases; Viral diseases. In: The Merck Manual of Diagnosis and Therapy, 17th Ed., Indianapolis, Wiley Publishers, 2005
  25. ↵
    Sirotin BZ, Keiser NP: On the history of the study of haemorrhagic fever with renal syndrome in eastern Russia. Nephrol Dial Transplant 16: 1288–1289, 2001
    OpenUrlCrossRefPubMed
  26. ↵
    Makela S, Ala-Houhala I, Mustonen J, Koivisto AM, Kouri T, Turjanmaa V, Vapalahti O, Vaheri A, Pasternack A: Renal function and blood pressure five years after Puumala virus-induced nephropathy. Kidney Int 58: 1711–1718, 2000
    OpenUrlCrossRefPubMed
  27. ↵
    Becker C, Goubeaud G, Zeier M, Zoller L: [Hantavirus infection and chronic renal failure]. Medizinische Welt 44: 569–573, 1993
    OpenUrl
  28. ↵
    Patnaik M, Velosa JA, Peter JB: Hantavirus-specific IgG, IgM, and IgA in acute and chronic renal disease versus congenital renal disease in the United States. Am J Kidney Dis 33: 734–737, 1999
    OpenUrlPubMed
  29. ↵
    Zeier M, Ritz E: [Hantavirus-induced acute renal failure]. Internist 37: 1092–1095, 1996
    OpenUrlPubMed
  30. ↵
    Zeier M, Zoller L, Haussmann W, Andrassy K, Ritz E: [The clinical picture and therapy of Hantaan virus infection]. Dtsch Med Wochenschr 115: 1678–1681, 1990
    OpenUrlPubMed
  31. ↵
    Mustonen J, Helin H, Pietila K, Brummer-Korvenkontio M, Hedman K, Vaheri A, Pasternack A: Renal biopsy findings and clinicopathologic correlations in nephropathia epidemica. Clin Nephrol 41: 121–126, 1994
    OpenUrlPubMed
  32. ↵
    Groen J, Bruijn JA, Gerding MN, Jordans JG, Moll van Charante AW, Osterhaus AD: Hantavirus antigen detection in kidney biopsies from patients with nephropathia epidemica. Clin Nephrol 46: 379–383, 1996
    OpenUrlPubMed
  33. ↵
    Death-Valley, US: Hantavirus: Technical information. Available: http://www.death-valley.us/hanta11.html. Accessed June 2005
  34. ↵
    Cebalo L, Dusek T, Kuzman I, Markotic A: Grading the severity of disease in patients with Puumala or Dobrava virus infections from 1995 to 2000 in Croatia. Acta Med Croatica 57: 355–359, 2003
    OpenUrlPubMed
  35. Mustonen J, Partanen J, Kanerva M, Pietila K, Vapalahti O, Pasternack A, Vaheri A: Genetic susceptibility to severe course of nephropathia epidemica caused by Puumala hantavirus. Kidney Int 49: 217–221, 1996
    OpenUrlCrossRefPubMed
  36. ↵
    Plyusnin A, Horling J, Kanerva M, Mustonen J, Cheng Y, Partanen J, Vapalahti O, Kukkonen SK, Niemimaa J, Henttonen H, Niklasson B, Lundkvist A, Vaheri A: Puumala hantavirus genome in patients with nephropathia epidemica: Correlation of PCR positivity with HLA haplotype and link to viral sequences in local rodents. J Clin Microbiol 35: 1090–1096, 1997
    OpenUrlAbstract/FREE Full Text
  37. ↵
    Kilpatrick ED, Terajima M, Koster FT, Catalina MD, Cruz J, Ennis FA: Role of specific CD8+ T cells in the severity of a fulminant zoonotic viral hemorrhagic fever, hantavirus pulmonary syndrome. J Immunol 172: 3297–3304, 2004
    OpenUrlAbstract/FREE Full Text
  38. ↵
    Yanagihara R, Silverman DJ: Experimental infection of human vascular endothelial cells by pathogenic and nonpathogenic hantaviruses. Arch Virol 111: 281–286, 1990
    OpenUrlCrossRefPubMed
  39. ↵
    Gavrilovskaya IN, Brown EJ, Ginsberg MH, Mackow ER: Cellular entry of hantaviruses which cause hemorrhagic fever with renal syndrome is mediated by beta3 integrins. J Virol 73: 3951–3959, 1999
    OpenUrlAbstract/FREE Full Text
  40. ↵
    Mackow ER, Gavrilovskaya IN: Cellular receptors and hantavirus pathogenesis. Curr Top Microbiol Immunol 256: 91–115, 2001
    OpenUrlCrossRefPubMed
  41. ↵
    Kanerva M, Mustonen J, Vaheri A: Pathogenesis of Puumala and other hantavirus infections. Rev Med Virol 8: 67–86, 1998
    OpenUrlCrossRefPubMed
  42. ↵
    Sundstrom JB, McMullan LK, Spiropoulou CF, Hooper WC, Ansari AA, Peters CJ, Rollin PE: Hantavirus infection induces the expression of RANTES and IP-10 without causing increased permeability in human lung microvascular endothelial cells. J Virol 75: 6070–6085, 2001
    OpenUrlAbstract/FREE Full Text
  43. ↵
    Kang JI, Park SH, Lee PW, Ahn BY: Apoptosis is induced by hantaviruses in cultured cells. Virology 264: 99–105, 1999
    OpenUrlCrossRefPubMed
  44. Li XD, Kukkonen S, Vapalahti O, Plyusnin A, Lankinen H, Vaheri A: Tula hantavirus infection of Vero E6 cells induces apoptosis involving caspase 8 activation. J Gen Virol 85: 3261–3268, 2004
    OpenUrlCrossRefPubMed
  45. Li XD, Lankinen H, Putkuri N, Vapalahti O, Vaheri A: Tula hantavirus triggers pro-apoptotic signals of ER stress in Vero E6 cells. Virology 333: 180–189, 2005
    OpenUrlCrossRefPubMed
  46. ↵
    Markotic A, Hensley L, Geisbert T, Spik K, Schmaljohn C: Hantaviruses induce cytopathic effects and apoptosis in continuous human embryonic kidney cells. J Gen Virol 84: 2197–2202, 2003
    OpenUrlCrossRefPubMed
  47. ↵
    Cosgriff TM, Lewis RM: Mechanisms of disease in hemorrhagic fever with renal syndrome. Kidney Int Suppl 35: S72–S79, 1991
    OpenUrlPubMed
  48. ↵
    Geimonen E, Neff S, Raymond T, Kocer SS, Gavrilovskaya IN, Mackow ER: Pathogenic and nonpathogenic hantaviruses differentially regulate endothelial cell responses. Proc Natl Acad Sci U S A 99: 13837–13842, 2002
    OpenUrlAbstract/FREE Full Text
  49. Khaiboullina SF, Rizvanov AA, Deyde VM, St Jeor SC: Andes virus stimulates interferon-inducible MxA protein expression in endothelial cells. J Med Virol 75: 267–275, 2005
    OpenUrlCrossRefPubMed
  50. Kraus AA, Raftery MJ, Giese T, Ulrich R, Zawatzky R, Hippenstiel S, Suttorp N, Kruger DH, Schonrich G: Differential antiviral response of endothelial cells after infection with pathogenic and nonpathogenic hantaviruses. J Virol 78: 6143–6150, 2004
    OpenUrlAbstract/FREE Full Text
  51. ↵
    Linderholm M, Ahlm C, Settergren B, Waage A, Tarnvik A: Elevated plasma levels of tumor necrosis factor (TNF)-alpha, soluble TNF receptors, interleukin (IL)-6, and IL-10 in patients with hemorrhagic fever with renal syndrome. J Infect Dis 173: 38–43, 1996
    OpenUrlCrossRefPubMed
  52. ↵
    Paakkala A, Mustonen J, Viander M, Huhtala H, Pasternack A: Complement activation in nephropathia epidemica caused by Puumala hantavirus. Clin Nephrol 53: 424–431, 2000
    OpenUrlPubMed
  53. ↵
    Khaiboullina SF, St Jeor SC: Hantavirus immunology. Viral Immunol 15: 609–625, 2002
    OpenUrlCrossRefPubMed
  54. ↵
    Linderholm M, Bjermer L, Juto P, Roos G, Sandstrom T, Settergren B, Tarnvik A: Local host response in the lower respiratory tract in nephropathia epidemica. Scand J Infect Dis 25: 639–646, 1993
    OpenUrlPubMed
  55. ↵
    Bostik P, Winter J, Ksiazek TG, Rollin PE, Villinger F, Zaki SR, Peters CJ, Ansari AA: Sin nombre virus (SNV) Ig isotype antibody response during acute and convalescent phases of hantavirus pulmonary syndrome. Emerg Infect Dis 6: 184–187, 2000
    OpenUrlPubMed
  56. ↵
    Groen J, Gerding M, Jordans JG, Clement JP, Osterhaus AD: Class and subclass distribution of Hantavirus-specific serum antibodies at different times after the onset of nephropathia epidemica. J Med Virol 43: 39–43, 1994
    OpenUrlPubMed
  57. ↵
    Alexeyev OA, Ahlm C, Billheden J, Settergren B, Wadell G, Juto P: Elevated levels of total and Puumala virus-specific immunoglobulin E in the Scandinavian type of hemorrhagic fever with renal syndrome. Clin Diagn Lab Immunol 1: 269–272, 1994
    OpenUrlAbstract/FREE Full Text
  58. ↵
    Borish L, Williams J, Johnson S, Mascali JJ, Miller R, Rosenwasser LJ: Anti-inflammatory effects of nedocromil sodium: Inhibition of alveolar macrophage function. Clin Exp Allergy 22: 984–990, 1992
    OpenUrlCrossRefPubMed
  59. ↵
    Clement J, McKenna P, Groen J, Osterhaus A, Colson P, Vervoort T, van der Groen G, Lee HW: Epidemiology and laboratory diagnosis of hantavirus (HTV) infections. Acta Clin Belg 50: 9–19, 1995
    OpenUrlPubMed
  60. ↵
    Lundkvist A, Horling J, Niklasson B: The humoral response to Puumala virus infection (nephropathia epidemica) investigated by viral protein specific immunoassays. Arch Virol 130: 121–130, 1993
    OpenUrlCrossRefPubMed
  61. ↵
    Lewis RM, Lee HW, See AF, Parrish DB, Moon JS, Kim DJ, Cosgriff TM: Changes in populations of immune effector cells during the course of haemorrhagic fever with renal syndrome. Trans R Soc Trop Med Hyg 85: 282–286, 1991
    OpenUrlCrossRefPubMed
  62. ↵
    de Carvalho Nicacio C, Sallberg M, Hultgren C, Lundkvist A: T-helper and humoral responses to Puumala hantavirus nucleocapsid protein: Identification of T-helper epitopes in a mouse model. J Gen Virol 82: 129–138, 2001
    OpenUrlCrossRefPubMed
  63. ↵
    Van Epps HL, Schmaljohn CS, Ennis FA: Human memory cytotoxic T-lymphocyte (CTL) responses to Hantaan virus infection: Identification of virus-specific and cross-reactive CD8(+) CTL epitopes on nucleocapsid protein. J Virol 73: 5301–5308, 1999
    OpenUrlAbstract/FREE Full Text
  64. ↵
    Makela S, Mustonen J, Ala-Houhala I, Hurme M, Koivisto AM, Vaheri A, Pasternack A: Urinary excretion of interleukin-6 correlates with proteinuria in acute Puumala hantavirus-induced nephritis. Am J Kidney Dis 43: 809–816, 2004
    OpenUrlCrossRefPubMed
  65. ↵
    Zaki SR, Greer PW, Coffield LM, Goldsmith CS, Nolte KB, Foucar K, Feddersen RM, Zumwalt RE, Miller GL, Khan AS, et al.: Hantavirus pulmonary syndrome. Pathogenesis of an emerging infectious disease. Am J Pathol 146: 552–579, 1995
    OpenUrlPubMed
  66. ↵
    Klingstrom J, Plyusnin A, Vaheri A, Lundkvist A: Wild-type Puumala hantavirus infection induces cytokines, C-reactive protein, creatinine, and nitric oxide in cynomolgus macaques. J Virol 76: 444–449, 2002
    OpenUrlAbstract/FREE Full Text
  67. ↵
    Temonen M, Mustonen J, Helin H, Pasternack A, Vaheri A, Holthofer H: Cytokines, adhesion molecules, and cellular infiltration in nephropathia epidemica kidneys: An immunohistochemical study. Clin Immunol Immunopathol 78: 47–55, 1996
    OpenUrlCrossRefPubMed
  68. ↵
    Terajima M, Vapalahti O, Van Epps HL, Vaheri A, Ennis FA: Immune responses to Puumala virus infection and the pathogenesis of nephropathia epidemica. Microbes Infect 6: 238–245, 2004
    OpenUrlCrossRefPubMed
  69. ↵
    Raftery MJ, Kraus AA, Ulrich R, Kruger DH, Schonrich G: Hantavirus infection of dendritic cells. J Virol 76: 10724–10733, 2002
    OpenUrlAbstract/FREE Full Text
  70. ↵
    Niikura M, Maeda A, Ikegami T, Saijo M, Kurane I, Morikawa S: Modification of endothelial cell functions by Hantaan virus infection: Prolonged hyper-permeability induced by TNF-alpha of Hantaan virus-infected endothelial cell monolayers. Arch Virol 149: 1279–1292, 2004
    OpenUrlPubMed
  71. ↵
    Kaukinen P, Vaheri A, Plyusnin A: Non-covalent interaction between nucleocapsid protein of Tula hantavirus and small ubiquitin-related modifier-1, SUMO-1. Virus Res 92: 37–45, 2003
    OpenUrlCrossRefPubMed
  72. Lee BH, Yoshimatsu K, Maeda A, Ochiai K, Morimatsu M, Araki K, Ogino M, Morikawa S, Arikawa J: Association of the nucleocapsid protein of the Seoul and Hantaan hantaviruses with small ubiquitin-like modifier-1-related molecules. Virus Res 98: 83–91, 2003
    OpenUrlCrossRefPubMed
  73. Li XD, Makela TP, Guo D, Soliymani R, Koistinen V, Vapalahti O, Vaheri A, Lankinen H: Hantavirus nucleocapsid protein interacts with the Fas-mediated apoptosis enhancer Daxx. J Gen Virol 83: 759–766, 2002
    OpenUrlCrossRefPubMed
  74. ↵
    Gavrilovskaya IN, Shepley M, Shaw R, Ginsberg MH, Mackow ER: Beta3 Integrins mediate the cellular entry of hantaviruses that cause respiratory failure. Proc Natl Acad Sci U S A 95: 7074–7079, 1998
    OpenUrlAbstract/FREE Full Text
  75. Gavrilovskaya IN, Peresleni T, Geimonen E, Mackow ER: Pathogenic hantaviruses selectively inhibit beta3 integrin directed endothelial cell migration. Arch Virol 147: 1913–1931, 2002
    OpenUrlCrossRefPubMed
  76. ↵
    Raymond T, Gorbunova E, Gavrilovskaya IN, Mackow ER: Pathogenic hantaviruses bind plexin-semaphorin-integrin domains present at the apex of inactive, bent alphavbeta3 integrin conformers. Proc Natl Acad Sci U S A 102: 1163–1168, 2005
    OpenUrlAbstract/FREE Full Text
  77. ↵
    Geimonen E, LaMonica R, Springer K, Farooqui Y, Gavrilovskaya IN, Mackow ER: Hantavirus pulmonary syndrome-associated hantaviruses contain conserved and functional ITAM signaling elements. J Virol 77: 1638–16343, 2003
  78. ↵
    Li Z, Bai X, Bian H: Serologic diagnosis of Hantaan virus infection based on a peptide antigen. Clin Chem 48: 645–647, 2002
    OpenUrlFREE Full Text
  79. ↵
    Hujakka H, Koistinen V, Kuronen I, Eerikainen P, Parviainen M, Lundkvist A, Vaheri A, Vapalahti O, Narvanen A: Diagnostic rapid tests for acute hantavirus infections: Specific tests for Hantaan, Dobrava and Puumala viruses versus a hantavirus combination test. J Virol Methods 108: 117–122, 2003
    OpenUrlCrossRefPubMed
  80. ↵
    Aitichou M, Saleh SS, McElroy AK, Schmaljohn C, Ibrahim MS: Identification of Dobrava, Hantaan, Seoul, and Puumala viruses by one-step real-time RT-PCR. J Virol Methods 124: 21–26, 2005
    OpenUrlCrossRefPubMed
  81. ↵
    Moreli ML, Sousa RL, Figueiredo LT: Detection of Brazilian hantavirus by reverse transcription polymerase chain reaction amplification of N gene in patients with hantavirus cardiopulmonary syndrome. Mem Inst Oswaldo Cruz 99: 633–638, 2004
    OpenUrlPubMed
  82. ↵
    Huggins JW, Kim GR, Brand OM, McKee KT Jr: Ribavirin therapy for Hantaan virus infection in suckling mice. J Infect Dis 153: 489–497, 1986
    OpenUrlCrossRefPubMed
  83. ↵
    Huggins JW, Hsiang CM, Cosgriff TM, Guang MY, Smith JI, Wu ZO, LeDuc JW, Zheng ZM, Meegan JM, Wang QN, et al.: Prospective, double-blind, concurrent, placebo-controlled clinical trial of intravenous ribavirin therapy of hemorrhagic fever with renal syndrome. J Infect Dis 164: 1119–1127, 1991
    OpenUrlCrossRefPubMed
  84. ↵
    Chapman LE, Mertz GJ, Peters CJ, Jolson HM, Khan AS, Ksiazek TG, Koster FT, Baum KF, Rollin PE, Pavia AT, Holman RC, Christenson JC, Rubin PJ, Behrman RE, Bell LJ, Simpson GL, Sadek RF; Ribavirin Study Group: Intravenous ribavirin for hantavirus pulmonary syndrome: Safety and tolerance during 1 year of open-label experience. Antivir Ther 4: 211–219, 1999
    OpenUrlPubMed
  85. ↵
    Chapman LE, Ellis BA, Koster FT, Sotir M, Ksiazek TG, Mertz GJ, Rollin PE, Baum KF, Pavia AT, Christenson JC, Rubin PJ, Jolson HM, Behrman RE, Khan AS, Bell LJ, Simpson GL, Hawk J, Holman RC, Peters CJ; Ribavirin Study Group: Discriminators between hantavirus-infected and -uninfected persons enrolled in a trial of intravenous ribavirin for presumptive hantavirus pulmonary syndrome. Clin Infect Dis 34: 293–304, 2002
    OpenUrlCrossRefPubMed
  86. ↵
    Severson WE, Schmaljohn CS, Javadian A, Jonsson CB: Ribavirin causes error catastrophe during Hantaan virus replication. J Virol 77: 481–488, 2003
  87. ↵
    Jonsson CB, Milligan BG, Arterburn JB: Potential importance of error catastrophe to the development of antiviral strategies for hantaviruses. Virus Res 107: 195–205, 2005
    OpenUrlCrossRefPubMed
  88. ↵
    Tamura M, Asada H, Kondo K, Takahashi M, Yamanishi K: Effects of human and murine interferons against hemorrhagic fever with renal syndrome (HFRS) virus (Hantaan virus). Antiviral Res 8: 171–178, 1987
    OpenUrlCrossRefPubMed
  89. ↵
    Frese M, Kochs G, Feldmann H, Hertkorn C, Haller O: Inhibition of bunyaviruses, phleboviruses, and hantaviruses by human MxA protein. J Virol 70: 915–923, 1996
    OpenUrlAbstract/FREE Full Text
  90. ↵
    Kanerva M, Melen K, Vaheri A, Julkunen I: Inhibition of Puumala and tula hantaviruses in Vero cells by MxA protein. Virology 224: 55–62, 1996
    OpenUrlCrossRefPubMed
  91. ↵
    Klingstrom J, Falk KI, Lundkvist A: Delayed viremia and antibody responses in Puumala hantavirus challenged passively immunized cynomolgus macaques. Arch Virol 150: 79–92, 2005
    OpenUrlCrossRefPubMed
  92. ↵
    Custer DM, Thompson E, Schmaljohn CS, Ksiazek TG, Hooper JW: Active and passive vaccination against hantavirus pulmonary syndrome with Andes virus M genome segment-based DNA vaccine. J Virol 77: 9894–9905, 2003
    OpenUrlAbstract/FREE Full Text
  93. ↵
    Bai WT, Xu ZK, Zhang FL, Luo W, Liu Y, Wu XA, Yan Y: Transient expression and characterization of intracellular single chain Fv against the nucleocapsid protein of Hantavirus. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 20: 705–707, 2004
    OpenUrlPubMed
  94. ↵
    Smee DF, Sidwell RW, Huffman JH, Huggins JW, Kende M, Verbiscar AJ: Antiviral activities of tragacanthin polysaccharides on Punta Toro virus infections in mice. Chemotherapy 42: 286–293, 1996
    OpenUrlPubMed
  95. ↵
    Chu F, Ji Q, Yan RM, Wang XM, Pei B: A study on detecting specific antibodies of hemorrhagic fever with renal syndrome and treatment with integrated traditional Chinese and western medicine. Zhong Xi Yi Jie He Xue Bao 2: 20–23, 2004
    OpenUrlPubMed
  96. ↵
    Cho HW, Howard CR: Antibody responses in humans to an inactivated hantavirus vaccine (Hantavax). Vaccine 17: 2569–2575, 1999
    OpenUrlCrossRefPubMed
  97. Cho HW, Howard CR, Lee HW: Review of an inactivated vaccine against hantaviruses. Intervirology 45: 328–333, 2002
    OpenUrlCrossRefPubMed
  98. ↵
    Hjelle B: Vaccines against hantaviruses. Expert Rev Vaccines 1: 373–384, 2002
    OpenUrlCrossRefPubMed
  99. ↵
    Park K, Kim CS, Moon KT: Protective effectiveness of hantavirus vaccine. Emerg Infect Dis 10: 2218–2220, 2004
    OpenUrlPubMed
  100. ↵
    Sohn YM, Rho HO, Park MS, Kim JS, Summers PL: Primary humoral immune responses to formalin inactivated hemorrhagic fever with renal syndrome vaccine (Hantavax): Consideration of active immunization in South Korea. Yonsei Med J 42: 278–284, 2001
    OpenUrlPubMed
  101. ↵
    Choi Y, Ahn CJ, Seong KM, Jung MY, Ahn BY: Inactivated Hantaan virus vaccine derived from suspension culture of Vero cells. Vaccine 21: 1867–1873, 2003
    OpenUrlCrossRefPubMed
  102. ↵
    Hooper JW, Li D: Vaccines against hantaviruses. Curr Top Microbiol Immunol 256: 171–191, 2001
    OpenUrlPubMed
  103. ↵
    Dargeviciute A, Brus Sjolander K, Sasnauskas K, Kruger DH, Meisel H, Ulrich R, Lundkvist A: Yeast-expressed Puumala hantavirus nucleocapsid protein induces protection in a bank vole model. Vaccine 20: 3523–3531, 2002
    OpenUrlCrossRefPubMed
  104. ↵
    Geldmacher A, Schmaler M, Kruger DH, Ulrich R: Yeast-expressed hantavirus Dobrava nucleocapsid protein induces a strong, long-lasting, and highly cross-reactive immune response in mice. Viral Immunol 17: 115–122, 2004
    OpenUrlCrossRefPubMed
  105. ↵
    Kehm R, Jakob NJ, Welzel TM, Tobiasch E, Viczian O, Jock S, Geider K, Sule S, Darai G: Expression of immunogenic Puumala virus nucleocapsid protein in transgenic tobacco and potato plants. Virus Genes 22: 73–83, 2001
    OpenUrlCrossRefPubMed
  106. ↵
    Khattak S, Darai G, Rosen-Wolff A: Puumala virus nucleocapsid protein expressed in transgenic plants is not immunogenic after oral administration. Virus Genes 29: 109–116, 2004
    OpenUrlCrossRefPubMed
  107. ↵
    Klingstrom J, Maljkovic I, Zuber B, Rollman E, Kjerrstrom A, Lundkvist A: Vaccination of C57/BL6 mice with Dobrava hantavirus nucleocapsid protein in Freund’s adjuvant induced partial protection against challenge. Vaccine 22: 4029–4034, 2004
    OpenUrlCrossRefPubMed
  108. ↵
    Geldmacher A, Skrastina D, Petrovskis I, Borisova G, Berriman JA, Roseman AM, Crowther RA, Fischer J, Musema S, Gelderblom HR, Lundkvist A, Renhofa R, Ose V, Kruger DH, Pumpens P, Ulrich R: An amino-terminal segment of hantavirus nucleocapsid protein presented on hepatitis B virus core particles induces a strong and highly cross-reactive antibody response in mice. Virology 323: 108–119, 2004
    OpenUrlCrossRefPubMed
  109. ↵
    Ulrich R, Koletzki D, Lachmann S, Lundkvist A, Zankl A, Kazaks A, Kurth A, Gelderblom HR, Borisova G, Meisel H, Kruger DH: New chimaeric hepatitis B virus core particles carrying hantavirus (serotype Puumala) epitopes: Immunogenicity and protection against virus challenge. J Biotechnol 73: 141–153, 1999
    OpenUrlCrossRefPubMed
  110. ↵
    Chu YK, Jennings GB, Schmaljohn CS: A vaccinia virus-vectored Hantaan virus vaccine protects hamsters from challenge with Hantaan and Seoul viruses but not Puumala virus. J Virol 69: 6417–6423, 1995
    OpenUrlAbstract/FREE Full Text
  111. Schmaljohn CS, Chu YK, Schmaljohn AL, Dalrymple JM: Antigenic subunits of Hantaan virus expressed by baculovirus and vaccinia virus recombinants. J Virol 64: 3162–3170, 1990
    OpenUrlAbstract/FREE Full Text
  112. ↵
    Yoshimatsu K, Yoo YC, Yoshida R, Ishihara C, Azuma I, Arikawa J: Protective immunity of Hantaan virus nucleocapsid and envelope protein studied using baculovirus-expressed proteins. Arch Virol 130: 365–376, 1993
    OpenUrlCrossRefPubMed
  113. ↵
    McClain DJ, Summers PL, Harrison SA, Schmaljohn AL, Schmaljohn CS: Clinical evaluation of a vaccinia-vectored Hantaan virus vaccine. J Med Virol 60: 77–85, 2000
    OpenUrlCrossRefPubMed
  114. ↵
    Bharadwaj M, Lyons CR, Wortman IA, Hjelle B: Intramuscular inoculation of Sin Nombre hantavirus cDNAs induces cellular and humoral immune responses in BALB/c mice. Vaccine 17: 2836–2843, 1999
    OpenUrlCrossRefPubMed
  115. Hooper JW, Kamrud KI, Elgh F, Custer D, Schmaljohn CS: DNA vaccination with hantavirus M segment elicits neutralizing antibodies and protects against Seoul virus infection. Virology 255: 269–278, 1999
    OpenUrlCrossRefPubMed
  116. Hooper JW, Custer DM, Thompson E, Schmaljohn CS: DNA vaccination with the Hantaan virus M gene protects Hamsters against three of four HFRS hantaviruses and elicits a high-titer neutralizing antibody response in Rhesus monkeys. J Virol 75: 8469–8477, 2001
    OpenUrlAbstract/FREE Full Text
  117. ↵
    Koletzki D, Schirmbeck R, Lundkvist A, Meisel H, Kruger DH, Ulrich R: DNA vaccination of mice with a plasmid encoding Puumala hantavirus nucleocapsid protein mimics the B-cell response induced by virus infection. J Biotechnol 84: 73–78, 2001
    OpenUrlCrossRefPubMed
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Journal of the American Society of Nephrology: 16 (12)
Journal of the American Society of Nephrology
Vol. 16, Issue 12
1 Dec 2005
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Hantavirus Infection
Walter Muranyi, Udo Bahr, Martin Zeier, Fokko J. van der Woude
JASN Dec 2005, 16 (12) 3669-3679; DOI: 10.1681/ASN.2005050561

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Hantavirus Infection
Walter Muranyi, Udo Bahr, Martin Zeier, Fokko J. van der Woude
JASN Dec 2005, 16 (12) 3669-3679; DOI: 10.1681/ASN.2005050561
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  • Article
    • Biology and Epidemiology
    • Clinical Course of NE, HFRS, and HPS
    • Pathology of NE, HFRS, and HPS
    • Pathophysiology of Hantavirus Infection
    • Laboratory Diagnosis of Hantavirus Infections
    • Therapy for Hantavirus Infections
    • Prevention of Hantavirus Infections
    • Conclusion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data Supps
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More in this TOC Section

  • Exercise in the End-Stage Renal Disease Population
  • Nephronophthisis-Associated Ciliopathies
  • Lipoprotein Metabolism and Lipid Management in Chronic Kidney Disease
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  • T-cell immunoglobulin and mucin (TIM) contributes to Hantaan virus entry into human airway epithelial cells
  • Ribosomal protein S19-binding domain provides insights into hantavirus nucleocapsid protein-mediated translation initiation mechanism
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