Up Front MattersBrief Reviews

Development of the Renal Arterioles

Maria Luisa S. Sequeira Lopez and R. Ariel Gomez
JASN December 2011, 22 (12) 2156-2165; DOI: https://doi.org/10.1681/ASN.2011080818

Abstract

The kidney is a highly vascularized organ that normally receives a fifth of the cardiac output. The unique spatial arrangement of the kidney vasculature with each nephron is crucial for the regulation of renal blood flow, GFR, urine concentration, and other specialized kidney functions. Thus, the proper and timely assembly of kidney vessels with their respective nephrons is a crucial morphogenetic event leading to the formation of a functioning kidney necessary for independent extrauterine life. Mechanisms that govern the development of the kidney vasculature are poorly understood. In this review, we discuss the anatomical development, embryological origin, lineage relationships, and key regulators of the kidney arterioles and postglomerular circulation. Because renal disease is associated with deterioration of the kidney microvasculature and/or the reenactment of embryonic pathways, understanding the morphogenetic events and processes that maintain the renal vasculature may open new avenues for the preservation of renal structure and function and prevent the progression of renal disease.

The mechanisms that govern the development of the kidney vasculature are poorly understood. In this brief review, we discuss the anatomical development, embryological origin, lineage relationships, and key regulators of the kidney arterioles and postglomerular circulation. For other important regulatory molecules and mechanisms already demonstrated for nonrenal vessels, as well as for the development of the glomerular capillaries, the reader is referred to some excellent reviews.1–14

In vivo, vascularization of the kidney is synchronized with epithelial nephrogenesis. Nephrogenesis of the definitive kidney results from the reciprocal inductive interaction between the primitive ureteric bud and the metanephric mesenchyme (Figure 1). The ureteric bud induces the mesenchyme to form tubular and glomerular epithelia. In turn, the surrounding mesenchyme induces the ureter to continue to grow and branch into the renal mesenchyme. As the ureter branches, the mesenchymal cells condense around each ureteric tip. The condensate develops into a vesicle, followed by a comma-shaped body that subsequently develops into an S-shaped body. Simultaneously, the glomerular cells differentiate until they acquire their adult features. In humans, nephrogenesis is complete by 34 to 35 weeks of gestation. In mice and rats, however, nephrogenesis continues after birth for about 3 to 7 days, respectively.

Figure 1.
Figure 1.

Nephrogenesis results from the interaction between the ureteric bud and the metanephric mesenchyme. Schematic of nephrogenesis. See text for details (adapted from reference 62).

Anatomical Development of the Renal Arterial Tree

Using microdissection techniques combined with histologic assessment, we studied the anatomical development of the renal arterial tree in mice and rats throughout embryonic and postnatal life, including adulthood (unpublished observations and Figure 2). Those studies reveal that in the mouse kidney, the first arterioles are seen around 15 to 16 days of gestation. By 18 to 19 days of gestation, there is a basic blueprint of arterial and arteriolar development.15 This is followed in the ensuing days by a burst of branching and elongation of new arterioles that repeat the basic pattern for about a week after birth, resulting in a remarkable increase in the complexity and surface area of the vasculature. These orchestrated series of events require that progenitor cells differentiate, acquire positional information, assemble in the right location within the vessel, and segregate those cells that participate in branching. When this process fails, the consequences are devastating as exemplified by the serious developmental defects described below, which occur (particularly in the newborn period when branching is at its peak) in animals and humans with ablation of renin cell precursors, mutations of the renin-angiotensin system, and lack of microRNAs in the renal vasculature, resulting in early arterial and arteriolar abnormalities that are followed by deterioration of kidney structure and function.15–23 In spite of its importance, very little is known about the fate of the vascular precursors and the mechanisms that lead them to differentiate and assemble into the kidney arterioles.

Figure 2.
Figure 2.

Increased complexity of the renal arterial tree during development. Microdissection of the entire preglomerular arteriolar tree at different developmental time points is shown at the same magnification.

Vascular Progenitors and Arteriolar Development

As mentioned above, for nephrons to function properly, each glomerulus must establish its own circulation. Blood enters the glomerulus through an afferent arteriole that is continued by glomerular capillaries where filtration occurs and leaves the glomerulus through an efferent arteriole (Figure 3). The establishment of these nephrovascular units is a remarkable morphogenetic event requiring spatial and temporal coordination of the cells destined to form these structures. Despite the critical relevance of this process, the actual events and molecules that control the formation of the kidney arterioles are unclear. At the time of the first division of the ureteric bud, within the metanephric mesenchyme (∼E12 mouse, E14 rat), the embryonic kidneys do not have arteriolar vessels. Whereas renal and extrarenal origins have been suggested for the renal vasculature, it is clear that progenitors are present in the metanephric kidney very early, well before vessels can be discerned. It is also clear that those precursors differentiate into all of the cell types necessary for the development of the kidney arterioles, including endothelial cells (ECs), smooth muscle cells (SMCs), and renin cells.24,25 Furthermore, cross-transplantation studies of those embryonic prevascular kidneys under the kidney capsule of a host mouse allowed us and others to demonstrate that precursor cells have the capacity to differentiate, acquire the right positional information, and fully assemble to form the kidney arterioles.24 The origin, lineage relationships, and morphogenesis of the kidney vasculature are also not well understood. Within the stromal compartment, we identified two putative and distinct early progenitor cells that give rise to all cells of the kidney arterioles and their perivascular compartment. Those progenitors are a precursor of hemogenic endothelium—provisionally called renal hemangioblasts—capable of giving rise to erythroid and ECs of the renal arteriole and a Foxd1+ cell from which all other arterial and perivascular/adventitial cells originate (Figure 3).

Figure 3.
Figure 3.

Two main progenitors give rise to the renal arteriole: the hemangioblast and the Foxd1+ progenitor cell. Solid arrows indicate current knowledge from others and our lab. Dashed arrows indicate possible lineage relationships. Phenotypic markers are indicated in gray. The renin cell precursor gives rise to a subset of vascular SMCs.

Hemogenic Progenitors.

It is now accepted that hematopoietic stem cells (HSCs) and ECs originate during embryogenesis from a common progenitor, the hemangioblast.26,27 These cells consist of a subpopulation of the primitive streak mesoderm that migrates to the yolk sac where they establish the primitive hematopoietic system. Primitive erythroid progenitors expand within the yolk sac and at embryonic day (E)8.5 enter the newly developing circulation and continue to mature. As soon as the liver starts to form, they home to it, where they complete their maturation and enucleate.28 On the other hand, definitive hematopoiesis is established within the embryo proper in the para-aortic splachnopleural region by regional hemangioblasts or hemogenic endothelium, which supports the development of HSCs. Primitive hematopoiesis generates mainly nucleated primitive erythrocytes, whereas definitive hematopoiesis generates all hematopoietic lineages, including nucleated and enucleated definitive erythrocytes and HSCs with a long-term repopulating activity. Using multiple techniques including Tie2.Cre;R26R lineage tracing, electron microscopy, laser capture microdissection, organ culture, and cross-transplantation experiments, we showed there is a lineage relationship between ECs and HSCs during embryonic development and the presence of HSCs budding from the endothelium.29 Recently, those studies were confirmed by others using an inducible VE-cadherin.Cre mouse line.30 Furthermore, time-lapse imaging with live markers and genetic analysis of differentiating ESCs also confirms that even non-aortic-derived ECs are hemogenic.31,32 Our studies also suggest the intrinsic capacity of the metanephros for hemo-vasculogenesis, the concomitant development of a blood vessel with its own blood.29 Our conceptualization of the contribution of hemogenic precursors to the development of the kidney arterioles is shown in Figure 3.

Progenitors of Mural Cells, Pericytes, and Interstitial Cells.

Foxd1 is a winged-helix transcription factor expressed in developing ventral diencephalon and in stromal cells of the developing kidney.33 Recently, we traced the lineage of Foxd1 cells within the kidney using a FoxD1.GFP.Cre knock-in mouse34 and found that Foxd1+ cells differentiate into renin cells, which in turn differentiate into a subset of vascular SMCs and mesangial cells, and the rest of SMCs, not originating from renin cells, mural/interstitial pericytes, and adventitial fibroblasts (unpublished, Figure 3). Whereas the lineage relationship among all of these cell types still needs to be examined in detail, those studies suggest that Foxd1+ cells are upstream in the differentiation pathway of mural cells of the arteriole.

Embryonic Origin of the Renal Vasculature: Renal, Extrarenal, or Both?

Whether the renal vessels originate from outside or within the kidney has been a long-standing controversial issue. Interspecies cross transplantation experiments of prevascular embryonic kidneys were originally performed into quail chorioallantoic membrane because of the presence of a characteristic quail nuclear marker that could easily track the contribution of the host tissue.25,35 Those experiments show the presence of host-derived ECs and mesangial cells within the embryonic kidney and suggest an extrarenal source for ECs and mesangial cells. The development of genetically-labeled mice with traceable reporters allowed further experiments in mice. When embryonic kidneys are transplanted into the anterior chamber of the eye or under the kidney capsule of adult hosts, the embryonic kidneys develop a proper vasculature with all of the vascular cell types (ECs, SMCs, and renin cells) originating from within the donor embryonic kidney (Figure 4).24,36 However, when prevascular embryonic kidneys are transplanted under the kidney capsule of newborn mice, cells derived from the host, still undergoing nephrogenesis, contribute to the ECs of developing vessels and glomeruli.36 Those studies suggest that when the embryonic kidney is transplanted into sites actively undergoing vascular development (such as the avian chorioallantoic membrane or the nephrogenic cortex of the newborn mouse), vascular precursors from the host respond to cues from the transplanted, developing metanephric mesenchyme, migrate, and differentiate into ECs and form part of the transplanted kidney vasculature. Thus, the presence, stage of development, and location of vascular progenitors determines where the vasculature originates. Within the embryo proper, the embryonic kidney is surrounded by a loose developing mesenchyme that also contains vascular precursors. It may be possible that some extrarenal vascular precursors originate from outside the embryonic kidney and migrate to form renal vessels. Therefore, renal vessels may have a dual, chimeric origin as discussed below.

Figure 4.
Figure 4.

The embryonic kidney possesses precursors for the renal arterioles. Embryonic kidney (embryonic day 12) section from a ROSA 26 mouse transplanted under the kidney capsule of an adult wild-type mouse for 7 days subjected to the 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-gal) reaction and immunostained for α-smooth muscle actin, which labels the SMCs of the arterioles and developing interstitial pericytes. Glomeruli were properly vascularized, and all of the vascular cell types originated from within the donor embryonic kidney.

Angiogenesis versus Vasculogenesis

Both angiogenesis and vasculogenesis have been described as major processes for vessel formation, and both are likely to occur during normal development as well as in pathologic conditions. Vasculogenesis consists in the local differentiation and assembly of endothelial precursors into endothelial tubes followed by recruitment of local mesenchymal cells that differentiate into SMCs, which coat the growing endothelial tube and provide stability to the newly formed arteriole. Angiogenesis is the formation of new vessels from preexisting ones and involves the sprouting, migration, and proliferation of already differentiated ECs followed by recruitment of perivascular cells along the way. Whereas both processes are markedly different, some studies support the notion that the kidney arterioles actually form by a combination of vasculogenesis and angiogenesis.37 We generated chimeric mice by injecting either ROSA26 ES cells (all cells are blue upon 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside [X-gal] reaction) into wild-type blastocysts or vice versa and examined their kidneys at different stages of development. By assessing the contribution of labeled versus unlabeled cells to a particular vessels or nephron segment, the technique permits us to infer its clonality or lack thereof. Our unpublished experiments suggest that the renal vasculature develops by a combination of both processes, with a prominence of vasculogenesis in the early embryonic kidney and a strong angiogenic component during the elongation of the vasculature at later stages. Interestingly, whereas the endothelial layer of the vessels appears to be clonal, the smooth muscle layer of the arterial tree is usually chimeric, indicating that differentiation of the smooth muscle wall occurs by vasculogenesis (Figure 5).

Figure 5.
Figure 5.

The renal arterial tree develops by vasculogenesis. This kidney section from a ROSA26/129S SvEv chimeric mouse subjected to the 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-gal) reaction shows a renal artery formed by contribution of both blastocyst and injected ESC-derived cells, suggesting a vasculogenic origin.

Although vasculogenesis and angiogenesis were originally thought to occur in the developing embryo, there now is evidence that these processes also occur during physiologic (endometrial angiogenesis) and pathologic (tumor vascularization) stresses of adult life. It is possible therefore that vasculogenesis and angiogenesis are activated during remodeling of the vasculature after pathologic conditions including ischemic renal injury.38

Regulation of Vascular Development

The major mechanisms that control vascular development in other systems have been the subject of excellent reviews1–14 and in the case of arteriolar development involve an active cross-talk between pericytes and ECs. Pericytes coat endothelial tubes, providing mechanical stability for the mature vessel, but they also participate in the regulation of blood flow. During arteriolar development, pericytes stimulate EC proliferation and migration. In turn, ECs activate the pericyte precursor cell population.11 The paracrine interactions between these two cell types involve vascular endothelial growth factor (VEGF), angiopoietins, PDGF-B, TGFβ, Notch, Ephrins, and sphingosine 1 phosphate (S1P) signaling pathways, among others. VEGF (A–D and placental growth factor) and its tyrosine kinase receptors are key regulators of vascular development (vasculogenesis, angiogenesis, and lymphangiogenesis). VEGF-A induces the migration and proliferation of pericytes and stimulates EC synthesis of nitric oxide, which also stimulates pericytes recruitment. Interestingly, pericytes and renin cells (closely related to pericytes) produce VEGF, which in turn may stabilize ECs in the renal growing arteriole.

The angiopoietin system also plays a critical role in vascular development. It is composed by two glycoproteins (angiopoietin-1 and -2) with opposite effects that bind to the endothelial cell-specific tyrosine-protein kinase receptor (TEK).39–41 Angiopoietin-1 mediates reciprocal interactions between the developing endothelium and perivascular cells. Deletion of angiopoietin-1 or TEK results in embryonic lethality caused by cardiovascular abnormalities. It has been suggested that the role of angiopoietin-1 in the maintenance of vascular integrity was by recruitment of pericytes surrounding the capillary tubes. However, a recent thorough study using inducible conditional deletion of angiopoietin-1 at different developmental stages and in different cell compartments demonstrates, when the deletion occurs between E10.5 and E12.5, that vascular abnormalities include an increase in the number and diameter of developing vessels, but recruitment of pericytes is not affected. Whereas deletion of angiopoietin-1 after E13.5 did not show an evident phenotype, in response to injury or microvascular stress there is an increase in angiogenesis and fibrosis resulting in an accelerated progression of the disease, resulting in more profound organ damage.42

PDGF-B is secreted by endothelial cells, which promotes pericyte proliferation by acting on PDGF-B receptor. In fact, mice deficient for PDGF-B or its receptor die in utero with widespread vascular defects consisting of vessel dilation and microaneurisms: sprouting capillaries in these null mice cannot attract pericytes.43,44 Furthermore, within the kidney, the lack of PDGF-B or its receptor fail to develop mesangial cells (which are a subtype of pericytes) and show abnormal glomerular capillaries.45

TGFβ signaling is also involved in vascular development. Both ECs and vascular SMCs express TGFβ receptors. Activation of this pathway results in the differentiation of mesenchymal progenitors to pericytes. Endothelial or vascular SMC deletion of TGFβ receptors results in early vascular defects and embryonic lethality.46

The S1P pathway is also a key regulator of EC cell migration and growth. S1P is a bioactive sphingolipid metabolite crucial in many biologic processes, including angiogenesis. S1P generated by two sphingosine kinases, SphK1 and SphK2, activates a family of five G-protein–coupled receptors, S1P1–S1P5. The variety of responses mediated by S1P receptors depends on the type of receptor and its coupled downstream effectors expressed in a given cell. Deletion of S1P1 in mice results in embryonic lethality at E12.5 to 14.5 caused by abnormal formation of blood vessels, possibly because of failure of migration and/or differentiation of vascular SMCs and pericytes, demonstrating the critical role of S1P1 in vascular maturation.47 However, the role of this pathway in the development of the renal vasculature has not yet been investigated.

Renin-Angiotensin in Kidney Vascular Development.

We have previously shown the association of renin-expressing cells with branching and elongation of the renal arterial tree.48 Development of a new arterial branch is preceded by the appearance of renin-expressing cells at the point of branching, followed by out pouching and elongation of a new arteriole covered almost exclusively with renin cell progenitors. As the vessels mature, renin cells differentiate into SMCs and mesangial cells. Finally when elongation is complete, a few renin cells remain near the glomerulus, the juxtaglomerular cells usually found in the adult animal. These experiments suggest that renin cells either directly or indirectly regulate the branching and elongation of the renal arterial tree through local generation of angiotensin. Support for this hypothesis is derived from separate experiments described below.

Treatment of rats during the first 12 days of postnatal life, when arteriolar branching is at its peak, with the AT1 blocker losartan results in marked impairment of kidney vascular development characterized by fewer, shorter, and thicker radial and afferent arterioles. This is accompanied by reduced glomerular size and tubular atrophy with attendant reduction in overall kidney growth. Interestingly, inhibition of angiotensin generation in rana catesbiana tadpoles undergoing prometamorphosis results in even more marked renal abnormalities with persistence of pronephric tubes and areas of undifferentiated mesenchyme.49 These experiments, as well as those of other investigators, indicate that not only is angiotensin II necessary for nephrovascular development but also its vascular growth actions are conserved across the phylogenetic scale.49 In agreement with those findings, deletion of any of the genes of the renin-angiotensin system (angiotensin-converting enzyme, renin, angiotensinogen, and AT1A+B) results in similar abnormalities (Figure 6). The growth effects of angiotensin II are not limited to the renal arterioles and extend to the postglomerular circulation. Recent work from Madsen et al.50 indicate that treatment with candesartan for 2 weeks reduces the length, volume, and surface area of capillaries in both the cortex and the medulla and inhibits the proper organization of vasa recta bundles. These findings are accompanied by inhibition of VEGF, angiopoietin-1 and -2, and the angiopoietin receptor Tie-2. As a result, medullary abnormalities ensue, encompassing hypoplasia of the papilla, thickening of vessels, and accumulation of αSMCs in the outer medullary interstitium. Functionally, this is reflected by a marked reduction in renal blood flow. As the authors suggest, it seems that postnatal development of the kidney depends on appropriate regulation of angiogenesis.

Figure 6.
Figure 6.

Gene deletion of any component of the renin angiotensin system results in renal arteriolar abnormalities. Representative Masson's Trichrome staining showing a marked increase in the size of the vascular wall and diminished lumen.

Role of the cAMP Pathway.

We have previously shown that cAMP signaling is necessary for renin cell identity and is also linked to development of kidney arterioles. Ligand binding of G-protein–coupled receptors results in activation of adenylyl cyclases, which in turn generate cAMP from ATP. cAMP activates protein kinase A, which in turn phosphorylates cAMP response element–binding (CREB) protein, a transcription factor that regulates the promoter activity of many genes, including the renin gene. Recently, in vitro and in vivo studies demonstrate the crucial role of the cAMP pathway on the acquisition and maintenance of the renin cell identity.51,52

Activation of the cAMP pathway was proven to be of importance not only for the well known stimulation of renin release but also for vascular development early in fetal life. Conditional deletion of Gαs in cells from the renin lineage such as arteriolar SMCs and mesangial cells results in almost complete absence of renin-expressing cells in the fetal kidney accompanied by blunted development of the preglomerular arterial tree.53 Thus, the cAMP pathway is an early regulator of the development and maturation of mural cells of the kidney. Whether the effect is direct in the SMC or as a result of diminished expression of renin and/or the presence of renin cells remains unclear. Interestingly, studies performed on β1/β2-adrenergic receptor-deficient mice show a marked reduction of renin expression in the vasculature in the embryo without changes in the number of juxtaglomerular cells in the adult, suggesting a distinct responsiveness to β-adrenergic stimulation between these two cell populations. The vasculature in those mice appears normal.54 Given that deletion of a more downstream element of the cAMP pathway, as in the case of Gαs, does impair vascular development, it seems that other mechanisms may compensate for the absence of the β receptors, whereas deletion of more proximal elements (with respect to the renin gene) of the cAMP pathway have a more profound phenotypic consequence. In fact, deletion of CBP and p300, two well known coactivators of CREB possessing histone acetyl transferase activity, results in severe vascular and nephron alterations accompanying a nearly complete depletion of renin cells. Overall, the aforementioned studies indicate that the cAMP pathway is fundamental in the differentiation of mural cells (renin and SMCs) of the renal arterioles.

Role of MicroRNAs in Vascular Development.

Endogenous miRNAs are small, noncoding RNAs of about 18 to 22 nucleotides in length. miRNAs exhibit tissue and cell specificity, and some are regulated developmentally. They control a myriad of cellular processes and are involved in cell differentiation and morphogenetic events. The generation of mature miRNAs requires the sequential processing of a looped primary transcript (pri-miR, 100-1000 nucleotides) to a single-stranded miR by two RNase III endonuclease complexes: Drosha-DGCR8 in the nucleus and Dicer in the cytoplasm. miRNAs mediate post-transcriptional events including mRNA degradation and translational repression. They may also have chromatin effects that ultimately influence gene expression.

Deletion of Dicer in renin cells results in a severe reduction in the number of renin-expressing cells accompanied by decreased circulating renin, hypotension, and striking nephrovascular abnormalities characterized by a unique type of striped cortico-medullary fibrosis.23 Within the fibrotic stripes, renal arterioles are unusually formed, replaced by interstitial cells in different states of development, phenotypic conversion, and /or degeneration.23 The mechanisms for the peculiar striped fibrosis remain to be studied, but it is likely to result from the combination of abnormal vessel architecture, which together with arterial hypotension leads to localized tissue hypoperfusion and ischemia along the path where the renal vessels traverse from the juxtamedullary area through the renal cortex (Figure 7).23

Figure 7.
Figure 7.

Schematic of possible mechanisms for the pathogenesis of striped renal fibrosis in mice with deletion of Dicer in the renin cell lineage (from reference 23).

Contrary to the phenotype encountered in mice with targeted deletions of the renin angiotensin system genes,17–22 in which SMCs proliferate around the blood vessel (Figure 6), Dicer−/− animals do not have arteriolar thickening, displaying instead adventitial fibroplasia.23 The reasons for the apparent decrease in SMCs is unlikely to be due to decreased renin because mice with homozygous deletion of the renin gene also have arteriolar thickening.17,18 The answer to this question is suggested from experiments in which the renin cells were ablated using diphtheria toxin targeted to the renin locus. Mice expressing diphtheria toxin A in renin cells have thin vessels and do not show hyperplasia of SMCs surrounding the arterioles (Figure 8).16 These experiments suggest that renin cells produce factors that stimulate the abnormal concentric growth of the kidney interlobular and afferent arterioles. In fact, recent gene profiling indicates that renin cells are capable of producing more than 14 different well known angiogenic and trophic factors, including VEGF, angiopoietin, IGF2, and autotaxin, among others (Figure 9).55 These factors may contribute in health to the branching and elongation of the renal arterial tree and in pathologic conditions to aberrant, circumferential growth of the renal arterioles as demonstrated in situations where there is an absence of angiotensin II actions in the presence of renin cell hyperplasia, ischemia, or exogenous activation of renal vasoconstrictors as described above.

Figure 8.
Figure 8.

Renin cells per se are responsible for the thickening of the arterial wall. (A) Kidney sections from mice with deletion of angiotensinogen (Atg−/−) and ablation of renin cells (DTA/DTA) immunostained for α-smooth muscle actin showing an enlargement and increase in thickness of the arteriolar wall in Atg−/−, whereas in the ones with ablation of renin cells, the vessel wall is not thickened (from reference 16). (B) Schematic showing the localization of renin cells (with dark dots) surrounding the thickened smooth muscle layer (red circles) of the arteriolar wall in the Atg−/−, whereas the DTA/DTA lacks renin cells and the smooth muscle layer remains thin.

Figure 9.
Figure 9.

The renin cell expresses angiogenic and trophic factors (circled in red).

Vascular Development and Renal Disease

In adults, the incidence of chronic renal disease is about 16%.56 The number of patients with end-stage renal disease continues to increase by 8 to 14% per year and is expected to double during the next 15 years. Many kidney diseases have either primary or secondary vascular lesions. Renal disease is associated with alterations in vascular remodeling, and progression of renal disease is accompanied by deterioration of kidney microvessels and development of glomerular and tubulointerstitial fibrosis.38,56 Thus, understanding the contribution of the pre- and postglomerular circulation to the progression of renal disease may help identify potential therapeutic targets. Alterations in the fate of vascular cells is a cause of renal interstitial fibrosis resulting from ureteral obstruction, a situation where pericytes and ECs phenotypically switch to become fibroblasts.34,57,58 However, other cells types participate in this process, including tubular cells, which have been shown to undergo epithelial-mesenchymal transition.59,60 The contribution of the resident interstitial fibroblast to renal fibrosis in models of vascular disease is unclear.

Vascular rarefaction and dysfunction in both small renal and systemic arteries also precedes renal end-organ damage in spontaneous models of hypertension-associated renal damage.61 It is unknown what mechanisms establish phenotypic variability in the vascular beds and how these might function for the maintenance of a healthy vascular system. When formation of the kidney vasculature is altered, the consequences are devastating, as exemplified by the serious developmental defects described above, occurring in animals and humans with ablation of renin cell precursors, mutations of the renin-angiotensin system, and a lack of miRNAs in the renal vasculature, resulting in early arterial and arteriolar abnormalities that are followed by the deterioration of kidney structure and function.15–23

Generation of new vessels in the kidney is not confined to embryonic development but seems to be a mechanism also activated in response to injury. Given the fact that a variety of renal diseases have abnormalities in the renal vasculature, understanding the mechanisms underlying the formation and maintenance of the renal vasculature is a logical step in developing strategies to preserve renal structure and function.

Future Opportunities

It is now more accepted that both ECs and HSCs derive from a common precursor cell, the hemangioblast. However, the mechanisms by which the early ECs and blood cells subsequently develop further diversity remain unknown. Some of these mechanisms are important for the maintenance of healthy blood and vascular systems and for injury repair in adult life. Thus, defining the mechanisms that regulate differentiation of this population of progenitor cells could lead to the discovery of new treatments for congenital and acquired blood and blood vessel diseases, including those related to the kidney and the systemic circulation. Thus, understanding the molecular cues for progenitor differentiation, vascular maturation, and branching are fundamental biologic questions with applicability to multiple renal and extrarenal disease processes. By providing new and fundamental knowledge regarding the mechanisms whereby progenitor cells contribute to the endowment of renal vascular cells and how those cells differentiate and assemble to form and maintain the renal arterioles, a frontier basically unexplored has the potential to benefit children and adults with congenital and acquired kidney diseases, vascular diseases, and hypertension.

DISCLOSURES

None.

Acknowledgments

This work was supported by National Institutes of Health Grants KO8 DK75481 (to M.L.S.S.L.) and R37 HL066242 and RO1 HL096735 (to R.A.G).

Footnotes

  • Published online ahead of print. Publication date available at www.jasn.org.

REFERENCES

  1. 1.↵
    1. Gaengel K,
    2. Genove G,
    3. Armulik A,
    4. Betsholtz C
    : Endothelial-mural cell signaling in vascular development and angiogenesis. Arterioscler Thromb Vasc Biol 29: 630–638, 2009
    OpenUrlAbstract/FREE Full Text
  2. 2.↵
    1. Gridley T
    : Notch signaling in vascular development and physiology. Development 134: 2709–2718, 2007
    OpenUrlAbstract/FREE Full Text
  3. 3.↵
    1. Kono M,
    2. Allende ML,
    3. Proia RL
    : Sphingosine-1-phosphate regulation of mammalian development. Biochim Biophys Acta 1781: 435–441, 2008
    OpenUrlPubMed
  4. 4.↵
    1. ten DP,
    2. Arthur HM
    : Extracellular control of TGFbeta signalling in vascular development and disease. Nat Rev Mol Cell Biol 8: 857–869, 2007
    OpenUrlCrossRefPubMed
  5. 5.↵
    1. Illi B,
    2. Colussi C,
    3. Rosati J,
    4. Spallotta F,
    5. Nanni S,
    6. Farsetti A,
    7. Capogrossi MC,
    8. Gaetano C
    : NO points to epigenetics in vascular development. Cardiovasc Res 90: 447–456, 2011
    OpenUrlAbstract/FREE Full Text
  6. 6.↵
    1. Coultas L,
    2. Chawengsaksophak K,
    3. Rossant J
    : Endothelial cells and VEGF in vascular development. Nature 438: 937–945, 2005
    OpenUrlCrossRefPubMed
  7. 7.↵
    1. Schulte-Merker S,
    2. Sabine A,
    3. Petrova TV
    : Lymphatic vascular morphogenesis in development, physiology, and disease. J Cell Biol 193: 607–618, 2011
    OpenUrlAbstract/FREE Full Text
  8. 8.↵
    1. Patel-Hett S,
    2. PA DA
    : Signal transduction in vasculogenesis and developmental angiogenesis. Int J Dev Biol 55: 353–363, 2011
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Caporali A,
    2. Emanueli C
    : MicroRNA regulation in angiogenesis. Vascul Pharmacol 2011, doi:10.1016/j.vph.2011.06.006
  10. 10.↵
    1. Carmeliet P,
    2. Jain RK
    : Molecular mechanisms and clinical applications of angiogenesis. Nature 473: 298–307, 2011
    OpenUrlCrossRefPubMed
  11. 11.↵
    1. Ribatti D,
    2. Nico B,
    3. Crivellato E
    : The role of pericytes in angiogenesis. Int J Dev Biol 55: 261–268, 2011
    OpenUrlCrossRefPubMed
  12. 12.↵
    1. Argraves KM,
    2. Wilkerson BA,
    3. Argraves WS
    : Sphingosine-1-phosphate signaling in vasculogenesis and angiogenesis. World J Biol Chem 1: 291–297, 2010
    OpenUrlCrossRefPubMed
  13. 13.↵
    1. Quaggin SE,
    2. Kreidberg JA
    : Development of the renal glomerulus: Good neighbors and good fences. Development 135: 609–620, 2008
    OpenUrlAbstract/FREE Full Text
  14. 14.↵
    1. Vaughan MR,
    2. Quaggin SE
    : How do mesangial and endothelial cells form the glomerular tuft? J Am Soc Nephrol 19: 24–33, 2008
    OpenUrlAbstract/FREE Full Text
  15. 15.↵
    1. Tomanek RJ
    1. Gomez RA,
    2. Sequeira-Lopez ML
    : Vascular development of the kidney. In: Assembly of the vasculature and its regulation, edited by Tomanek RJ , Boston, Birkhauser, 2002, pp 193–210
  16. 16.↵
    1. Pentz ES,
    2. Moyano MA,
    3. Thornhill BA,
    4. Sequeira Lopez ML,
    5. Gomez RA
    : Ablation of renin-expressing juxtaglomerular cells results in a distinct kidney phenotype. Am J Physiol Regul Integr Comp Physiol 286: R474–R483, 2004
    OpenUrlAbstract/FREE Full Text
  17. 17.↵
    1. Yanai K,
    2. Saito T,
    3. Kakinuma Y,
    4. Kon Y,
    5. Hirota K,
    6. Taniguchi-Yanai K,
    7. Nishijo N,
    8. Shigematsu Y,
    9. Horiguchi H,
    10. Kasuya Y,
    11. Sugiyama F,
    12. Yagami K,
    13. Murakami K,
    14. Fukamizu A
    : Renin-dependent cardiovascular functions and renin-independent blood-brain barrier functions revealed by renin-deficient mice. J Biol Chem 275: 5–8, 2000
    OpenUrlAbstract/FREE Full Text
  18. 18.↵
    1. Takahashi N,
    2. Lopez ML,
    3. Cowhig JE Jr.,
    4. Taylor MA,
    5. Hatada T,
    6. Riggs E,
    7. Lee G,
    8. Gomez RA,
    9. Kim HS,
    10. Smithies O
    : Ren1c homozygous null mice are hypotensive and polyuric, but heterozygotes are indistinguishable from wild-type. J Am Soc Nephrol 16: 125–132, 2005
    OpenUrlAbstract/FREE Full Text
  19. 19.↵
    1. Xu D,
    2. Borges GR,
    3. Grobe JL,
    4. Pelham CJ,
    5. Yang B,
    6. Sigmund CD
    : Preservation of intracellular renin expression is insufficient to compensate for genetic loss of secreted renin. Hypertension 54: 1240–1247, 2009
    OpenUrlAbstract/FREE Full Text
  20. 20.↵
    1. Kim HS,
    2. Krege JH,
    3. Kluckman KD,
    4. Hagaman JR,
    5. Hodgin JB,
    6. Best CF,
    7. Jennette JC,
    8. Coffman TM,
    9. Maeda N,
    10. Smithies O
    : Genetic control of blood pressure and the angiotensinogen locus. Proc Natl Acad Sci U S A 92: 2735–2739, 1995
    OpenUrlAbstract/FREE Full Text
  21. 21.↵
    1. Hilgers KF,
    2. Reddi V,
    3. Krege JH,
    4. Smithies O,
    5. Gomez RA
    : Aberrant renal vascular morphology and renin expression in mutant mice lacking angiotensin-converting enzyme. Hypertension 29: 216–221, 1997
    OpenUrlAbstract/FREE Full Text
  22. 22.↵
    1. Tsuchida S,
    2. Matsusaka T,
    3. Chen X,
    4. Okubo S,
    5. Niimura F,
    6. Nishimura H,
    7. Fogo A,
    8. Utsunomiya H,
    9. Inagami T,
    10. Ichikawa I
    : Murine double nullizygotes of the angiotensin type 1A and 1B receptor genes duplicate severe abnormal phenotypes of angiotensinogen nullizygotes. J Clin Invest 101: 755–760, 1998
    OpenUrlCrossRefPubMed
  23. 23.↵
    1. Sequeira-Lopez ML,
    2. Weatherford ET,
    3. Borges GR,
    4. Monteagudo MC,
    5. Pentz ES,
    6. Harfe BD,
    7. Carretero O,
    8. Sigmund CD,
    9. Gomez RA
    : The microRNA-processing enzyme dicer maintains juxtaglomerular cells. J Am Soc Nephrol 20: 460–461, 2010
    OpenUrl
  24. 24.↵
    1. Sequeira Lopez ML,
    2. Pentz ES,
    3. Robert B,
    4. Abrahamson DR,
    5. Gomez RA
    : Embryonic origin and lineage of juxtaglomerular cells. Am J Physiol Renal Physiol 281: F345–F356, 2001
    OpenUrlAbstract/FREE Full Text
  25. 25.↵
    1. Hyink DP,
    2. Tucker DC,
    3. St John PL,
    4. Leardkamolkarn V,
    5. Accavitti MA,
    6. Abrass CK,
    7. Abrahamson DR
    : Endogenous origin of glomerular endothelial and mesangial cells in grafts of embryonic kidneys. Am J Physiol 270: F886–F899, 1996
    OpenUrlPubMed
  26. 26.↵
    1. Lancrin C,
    2. Sroczynska P,
    3. Serrano AG,
    4. Gandillet A,
    5. Ferreras C,
    6. Kouskoff V,
    7. Lacaud G
    : Blood cell generation from the hemangioblast. J Mol Med 88: 167–172, 2010
    OpenUrlCrossRefPubMed
  27. 27.↵
    1. Sabin F
    : Origin and development of the primitive vessels of the chick and the pig. Contrib Embryol 6: 61–124, 1917
    OpenUrl
  28. 28.↵
    1. Isern J,
    2. Fraser ST,
    3. He Z,
    4. Baron MH
    : Developmental niches for embryonic erythroid cells. Blood Cells Mol Dis 44: 207–208, 2010
    OpenUrlCrossRefPubMed
  29. 29.↵
    1. Sequeira Lopez ML,
    2. Chernavvsky DR,
    3. Nomasa T,
    4. Wall L,
    5. Yanagisawa M,
    6. Gomez RA
    : The embryo makes red blood cell progenitors in every tissue simultaneously with blood vessel morphogenesis. Am J Physiol Regul Integr Comp Physiol 284: R1126–R1137, 2003
    OpenUrlAbstract/FREE Full Text
  30. 30.↵
    1. Zovein AC,
    2. Hofmann JJ,
    3. Lynch M,
    4. French WJ,
    5. Turlo KA,
    6. Yang Y,
    7. Becker MS,
    8. Zanetta L,
    9. Dejana E,
    10. Gasson JC,
    11. Tallquist MD,
    12. Iruela-Arispe ML
    : Fate tracing reveals the endothelial origin of hematopoietic stem cells. Cell Stem Cell 3: 625–636, 2008
    OpenUrlCrossRefPubMed
  31. 31.↵
    1. Lancrin C,
    2. Sroczynska P,
    3. Stephenson C,
    4. Allen T,
    5. Kouskoff V,
    6. Lacaud G
    : The haemangioblast generates haematopoietic cells through a haemogenic endothelium stage. Nature 457: 892–895, 2009
    OpenUrlCrossRefPubMed
  32. 32.↵
    1. Chen MJ,
    2. Yokomizo T,
    3. Zeigler BM,
    4. Dzierzak E,
    5. Speck NA
    : Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter. Nature 457: 887–891, 2009
    OpenUrlCrossRefPubMed
  33. 33.↵
    1. Hatini V,
    2. Huh SO,
    3. Herzlinger D,
    4. Soares VC,
    5. Lai E
    : Essential role of stromal mesenchyme in kidney morphogenesis revealed by targeted disruption of winged helix transcription factor BF-2. Genes Dev 10: 1467–1478, 1996
    OpenUrlAbstract/FREE Full Text
  34. 34.↵
    1. Humphreys BD,
    2. Lin SL,
    3. Kobayashi A,
    4. Hudson TE,
    5. Nowlin BT,
    6. Bonventre JV,
    7. Valerius MT,
    8. McMahon AP,
    9. Duffield JS
    : Fate tracing reveals the pericyte and not epithelial origin of myofibroblasts in kidney fibrosis. Am J Pathol 176: 85–97, 2010
    OpenUrlCrossRefPubMed
  35. 35.↵
    1. Sariola H,
    2. Ekblom P,
    3. Lehtonen E,
    4. Saxen L
    : Differentiation and vascularization of the metanephric kidney grafted on the chorioallantoic membrane. Dev Biol 96: 427–435, 1983
    OpenUrlCrossRefPubMed
  36. 36.↵
    1. Robert B,
    2. St John PL,
    3. Hyink DP,
    4. Abrahamson DR
    : Evidence that embryonic kidney cells expressing flk-1 are intrinsic, vasculogenic angioblasts. Am J Physiol 271: F744–F753, 1996
    OpenUrlPubMed
  37. 37.↵
    1. Gattone VH,
    2. Goldowitz D
    : The renal glomerulus and vasculature in “aggregation” chimeric mice. Nephron 90: 267–272, 2002
    OpenUrlCrossRefPubMed
  38. 38.↵
    1. Chade AR
    : Renovascular disease, microcirculation, and the progression of renal injury: Role of angiogenesis. Am J Physiol Regul Integr Comp Physiol 300: R783–R790, 2011
    OpenUrlAbstract/FREE Full Text
  39. 39.↵
    1. Davis S,
    2. Aldrich TH,
    3. Jones PF,
    4. Acheson A,
    5. Compton DL,
    6. Jain V,
    7. Ryan TE,
    8. Bruno J,
    9. Radziejewski C,
    10. Maisonpierre PC,
    11. Yancopoulos GD
    : Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning. Cell 87: 1161–1169, 1996
    OpenUrlCrossRefPubMed
  40. 40.↵
    1. Maisonpierre PC,
    2. Suri C,
    3. Jones PF,
    4. Bartunkova S,
    5. Wiegand SJ,
    6. Radziejewski C,
    7. Compton D,
    8. McClain J,
    9. Aldrich TH,
    10. Papadopoulos N,
    11. Daly TJ,
    12. Davis S,
    13. Sato TN,
    14. Yancopoulos GD
    : Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science 277: 55–60, 1997
    OpenUrlAbstract/FREE Full Text
  41. 41.↵
    1. Scharpfenecker M,
    2. Fiedler U,
    3. Reiss Y,
    4. Augustin HG
    : The Tie-2 ligand angiopoietin-2 destabilizes quiescent endothelium through an internal autocrine loop mechanism. J Cell Sci 118: 771–780, 2005
    OpenUrlAbstract/FREE Full Text
  42. 42.↵
    1. Jeansson M,
    2. Gawlik A,
    3. Anderson G,
    4. Li C,
    5. Kerjaschki D,
    6. Henkelman M,
    7. Quaggin SE
    : Angiopoietin-1 is essential in mouse vasculature during development and in response to injury. J Clin Invest 121: 2278–2289, 2011
    OpenUrlCrossRefPubMed
  43. 43.↵
    1. Leveen P,
    2. Pekny M,
    3. Gebre-Medhin S,
    4. Swolin B,
    5. Larsson E,
    6. Betsholtz C
    : Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities. Genes Dev 8: 1875–1887, 1994
    OpenUrlAbstract/FREE Full Text
  44. 44.↵
    1. Lindblom P,
    2. Gerhardt H,
    3. Liebner S,
    4. Abramsson A,
    5. Enge M,
    6. Hellstrom M,
    7. Backstrom G,
    8. Fredriksson S,
    9. Landegren U,
    10. Nystrom HC,
    11. Bergstrom G,
    12. Dejana E,
    13. Ostman A,
    14. Lindahl P,
    15. Betsholtz C
    : Endothelial PDGF-B retention is required for proper investment of pericytes in the microvessel wall. Genes Dev 17: 1835–1840, 2003
    OpenUrlAbstract/FREE Full Text
  45. 45.↵
    1. Lindahl P,
    2. Hellstrom M,
    3. Kalen M,
    4. Karlsson L,
    5. Pekny M,
    6. Pekna M,
    7. Soriano P,
    8. Betsholtz C
    : Paracrine PDGF-B/PDGF-Rbeta signaling controls mesangial cell development in kidney glomeruli. Development 125: 3313–3322, 1998
    OpenUrlAbstract
  46. 46.↵
    1. Carvalho RL,
    2. Itoh F,
    3. Goumans MJ,
    4. Lebrin F,
    5. Kato M,
    6. Takahashi S,
    7. Ema M,
    8. Itoh S,
    9. van RM,
    10. Bertolino P,
    11. ten DP,
    12. Mummery CL
    : Compensatory signalling induced in the yolk sac vasculature by deletion of TGFbeta receptors in mice. J Cell Sci 120: 4269–4277, 2007
    OpenUrlAbstract/FREE Full Text
  47. 47.↵
    1. Liu Y,
    2. Wada R,
    3. Yamashita T,
    4. Mi Y,
    5. Deng CX,
    6. Hobson JP,
    7. Rosenfeldt HM,
    8. Nava VE,
    9. Chae SS,
    10. Lee MJ,
    11. Liu CH,
    12. Hla T,
    13. Spiegel S,
    14. Proia RL
    : Edg-1, the G protein-coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation. J Clin Invest 106: 951–961, 2000
    OpenUrlCrossRefPubMed
  48. 48.↵
    1. Reddi V,
    2. Zaglul A,
    3. Pentz ES,
    4. Gomez RA
    : Renin-expressing cells are associated with branching of the developing kidney vasculature. J Am Soc Nephrol 9: 63–71, 1998
    OpenUrlAbstract
  49. 49.↵
    1. Tufro-McReddie A,
    2. Romano LM,
    3. Harris JM,
    4. Ferder L,
    5. Gomez RA
    : Angiotensin II regulates nephrogenesis and renal vascular development. Am J Physiol 269: F110–F115, 1995
    OpenUrlPubMed
  50. 50.↵
    1. Madsen K,
    2. Marcussen N,
    3. Pedersen M,
    4. Kjaersgaard G,
    5. Facemire C,
    6. Coffman TM,
    7. Jensen BL
    : Angiotensin II promotes development of the renal microcirculation through AT1 receptors. J Am Soc Nephrol 21: 448–459, 2010
    OpenUrlAbstract/FREE Full Text
  51. 51.↵
    1. Pentz ES,
    2. Sequeira Lopez ML,
    3. Cordaillat M,
    4. Gomez RA
    : Identity of the renin cell is mediated by cAMP and chromatin remodeling: An in vitro model for studying cell recruitment and plasticity. Am J Physiol Heart Circ Physiol 294: H699–H707, 2008
    OpenUrlAbstract/FREE Full Text
  52. 52.↵
    1. Gomez RA,
    2. Pentz ES,
    3. Jin X,
    4. Cordaillat M,
    5. Sequeira Lopez ML
    : CBP and p300 are essential for renin cell identity and morphological integrity of the kidney. Am J Physiol Heart Circ Physiol 296: H1255–H1262, 2009
    OpenUrlAbstract/FREE Full Text
  53. 53.↵
    1. Neubauer B,
    2. Machura K,
    3. Chen M,
    4. Weinstein LS,
    5. Oppermann M,
    6. Sequeira-Lopez ML,
    7. Gomez RA,
    8. Schnermann J,
    9. Castrop H,
    10. Kurtz A,
    11. Wagner C
    : Development of vascular renin expression in the kidney critically depends on the cyclic AMP pathway. Am J Physiol Renal Physiol 296: F1006–F1012, 2009
    OpenUrlAbstract/FREE Full Text
  54. 54.↵
    1. Neubauer B,
    2. Machura K,
    3. Schnermann J,
    4. Wagner C
    : Renin expression in large renal vessels during fetal development depends on functional {beta}1/{beta}2-adrenergic receptors. Am J Physiol Renal Physiol 301: F71–F77, 2011
    OpenUrlAbstract/FREE Full Text
  55. 55.↵
    1. Brunskill EW,
    2. Sequeira-Lopez MLS,
    3. Pentz ES,
    4. Lin E,
    5. Yu J,
    6. Aronow BJ,
    7. Potter SS,
    8. Gomez RA
    : Genes that confer the identity of the renin cell. J Am Soc Nephrol 22: 2213–2225, 2011
    OpenUrlAbstract/FREE Full Text
  56. 56.↵
    1. Lerman LO,
    2. Chade AR
    : Angiogenesis in the kidney: A new therapeutic target? Curr Opin Nephrol Hypertens 18: 160–165, 2009
    OpenUrlCrossRefPubMed
  57. 57.↵
    1. Lin SL,
    2. Kisseleva T,
    3. Brenner DA,
    4. Duffield JS
    : Pericytes and perivascular fibroblasts are the primary source of collagen-producing cells in obstructive fibrosis of the kidney. Am J Pathol 173: 1617–1627, 2008
    OpenUrlCrossRefPubMed
  58. 58.↵
    1. Zeisberg EM,
    2. Potenta SE,
    3. Sugimoto H,
    4. Zeisberg M,
    5. Kalluri R
    : Fibroblasts in kidney fibrosis emerge via endothelial-to-mesenchymal transition. J Am Soc Nephrol 19: 2282–2287, 2008
    OpenUrlAbstract/FREE Full Text
  59. 59.↵
    1. Iwano M,
    2. Plieth D,
    3. Danoff TM,
    4. Xue C,
    5. Okada H,
    6. Neilson EG
    : Evidence that fibroblasts derive from epithelium during tissue fibrosis. J Clin Invest 110: 341–350, 2002
    OpenUrlCrossRefPubMed
  60. 60.↵
    1. Zeisberg M,
    2. Neilson EG
    : Mechanisms of tubulointerstitial fibrosis. J Am Soc Nephrol 21: 1819–1834, 2010
    OpenUrlAbstract/FREE Full Text
  61. 61.↵
    1. Ochodnicky P,
    2. Henning RH,
    3. Buikema HJ,
    4. de ZD,
    5. Provoost AP,
    6. van Dokkum RP
    : Renal vascular dysfunction precedes the development of renal damage in the hypertensive Fawn-Hooded rat. Am J Physiol Renal Physiol 298: F625–F633, 2010
    OpenUrlAbstract/FREE Full Text
  62. 62.↵
    1. Li W,
    2. Kessler P,
    3. Williams BR
    : Transcript profiling of Wilms tumors reveals connections to kidney morphogenesis and expression patterns associated with anaplasia. Oncogene 24: 457–468, 2005
    OpenUrlCrossRefPubMed
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Development of the Renal Arterioles
Maria Luisa S. Sequeira Lopez, R. Ariel Gomez
JASN Dec 2011, 22 (12) 2156-2165; DOI: 10.1681/ASN.2011080818
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