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Up Front MattersBrief Reviews
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Body Fluid Dynamics: Back to the Future

Gautam Bhave and Eric G. Neilson
JASN December 2011, 22 (12) 2166-2181; DOI: https://doi.org/10.1681/ASN.2011080865
Gautam Bhave
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Eric G. Neilson
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Abstract

Pioneering investigations conducted over a half century ago on tonicity, transcapillary fluid exchange, and the distribution of water and solute serve as a foundation for understanding the physiology of body fluid spaces. With passage of time, however, some of these concepts have lost their connectivity to more contemporary information. Here we examine the physical forces determining the compartmentalization of body fluid and its movement across capillary and cell membrane barriers, drawing particular attention to the interstitium operating as a dynamic interface for water and solute distribution rather than as a static reservoir. Newer work now supports an evolving model of body fluid dynamics that integrates exchangeable Na+ stores and transcapillary dynamics with advances in interstitial matrix biology.

Transcapillary movement of water and solute between cells and extracellular compartments interfaces with powerful physical forces residing in the interstitial matrix. A modern view of body fluid spaces hinges on reconnecting historical principles with this new and emerging dynamic.

Compartmentalization of Body Water

The content of total body water (TBW) is a physiologic function of tissue composition leading to qualitatively predictable alterations with age, gender, and body weight. Most tissues such as skin, muscle, visceral organs, and brain consist of 70 to 80% water by weight, whereas adipose tissue and bone are only 10 to 20% water. TBW reflects a weighted average of tissue water content with relatively lower values in subjects with greater adiposity or lower muscle mass. Relative to weight, women and elderly individuals generally have less body water because of higher content of body fat or preferential loss of muscle mass with age, respectively. TBW increases with obesity but decreases relative to body weight with the gain of relatively drier adipose tissue.1,2

Nephrologists routinely estimate TBW to gauge electrolyte and fluid deficits with hypovolemia or hypertonicity, assess dialytic adequacy using TBW as a surrogate for the volume of distribution of urea, guide drug dosing, and rationalize dialytic clearance of toxins. TBW is classically estimated as 60% of body weight in men and 50% of body weight in women deducting 5% for elderly patients.3 Given physiologic variation in body tissue composition, early investigators recognized that absolute weight-based rules of estimation apply only to a select population of healthy individuals4 and subsequently derived regression equations better predict TBW in a broader range of subjects. Anthropomorphic equations including age, gender, ethnicity, weight, and height are now available to improve the accuracy of estimation (see supplemental material).5–7

A simple approach to compartmentalization divides body water into extracellular fluid (ECF) and intracellular fluid (ICF) domains (Figure 1). ECF is subdivided into five subcompartments: plasma volume; interstitial and lymph fluid; dense connective tissue and bone; transcellular fluid within body cavities such as the pleural space, cerebrospinal fluid system, peritoneal cavity, and recirculating ductal secretions from the gastrointestinal tract; and adipose tissue.2,8 Scant adipose tissue water resides primarily in the ECF and tips compartmentalization toward the extracellular compartment with increasing obesity. Higher fat mass and lower skeletal muscle content raises relative ECF to 45 to 48% of TBW in women, whereas the converse shifts ECF closer to 42 to 45% of TBW in men. Morbid obesity may result in the relative expansion of ECF up to 50 to 60% of TBW.4,8–10

Figure 1.
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Figure 1.

Body fluid compartments. In the “average” adult man, intracellular fluid (ICF) and extracellular fluid (ECF) domains consist of about 57 and 43% of total body water (TBW). The ECF compartment is further subdivided into interstitial fluid (ISF)/lymph, plasma, bone and connective tissue, adipose tissue, and transcellular water. Skeletal muscle predominates the ICF. Percentages are percent of TBW. Adapted from references 2 and 27. RBCs, red blood cells.

No experimental technique perfectly measures ECF or its components. The distribution volume of saccharides (inulin, sucrose, and mannitol), anions (thiocyanate, thiosulfate, sulfate, bromide, and chloride), and sodium has been used to estimate ECF water. These volumes encompass different anatomic and functional spaces (Table 1).2,11–14 When corrected for intracellular entry (approximately 10%), the distribution volume of halides best estimates ECF at approximately 42 to 48% of TBW depending on age and gender (approximately 45% as a population average).4,11,14,15 Delineating the exact distribution of ECF among its compartments requires an integration of classic tracer distribution volumes with anatomic data including classic human dissection and chemical tissue analysis or more recent imaging techniques.16 Consistent estimates of plasma, interstitial, transcellular, and adipose ECF volumes have been delineated despite varying data sources over time (Figure 1).2,4,14,16,17 Dense connective tissue and bone pose technical difficulties, particularly with the accurate separation of connective tissue, muscle, and marrow from bone, variation in bone preparation (± marrow, ± articular cartilage, cortical, or trabecular bone), and significant loss of water with age.14,18 Edelman and Leibman2 drew upon historic dissection data for skeletal water as 16% of body weight,19 skeletal water content as 25 to 30% by weight,20–23 and assumed skeletal water is >90% extracellular to calculate ECF bone water as 7.5% of TBW. Unfortunately, skeletal water is not >90% extracellular, because skeletal weight includes bone and enclosed marrow (Table 2).17,18,24,25 Normally active bone marrow is highly cellular, predominantly intracellular water.25,26 When bone and marrow are accounted for, skeletal water is only about 60% extracellular, reducing bone ECF to 3% of TBW (Table 2).

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Table 1.

Tracer distributions for measurement of ECF volume

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Table 2.

Distribution of water and Na+ within bone and marrow (skeleton)

Skeletal muscle water is often underappreciated but accounts for 40 to 50% of TBW, nearly 75% of ICF and cell mass, and about 33% of interstitial fluid volume. Loss of muscle mass redistributes TBW from ICF to ECF and increases the ratio of plasma to interstitial fluid volume.4,27 To clarify the compartmentalization of body water, we provide a balance sheet for body weight and water distribution among the major body tissues for men and women (Table 3).

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Table 3.

Body water distribution among tissues

Forces Governing Water Distribution

Hydrostatic pressure and osmosis drive water between the ECF and ICF compartments, and the eventual body water distribution reflects a steady state of these forces28: Embedded Image Pic and Pec represent intracellular and extracellular hydrostatic pressures, respectively, and Πic and Πec represent osmotic pressures. Plants, fungi, and bacteria possess rigid cell walls that generate hydrostatic pressure to counteract osmotic pressure gradients. Animal cells shed these reinforced walls during evolution to gain flexibility, but the cost of this loss leaves cell volume regulation to the mercy of extracellular tonicity29,30: Embedded Image Embedded Image Embedded Image Embedded Image where [c] represents molal solute concentration, z is the valence for electrolyte solutes, and ϕ is the osmotic coefficient for nonideality (see supplemental material for further details and index of mathematical symbols).31,32

Osmotic water movement is directly proportional to hydraulic permeability (Lp) and solute concentration gradient (Δ[c]) in the ideal situation of an impermeable solute33: Embedded Image Most solutes are partially permeable and undergo convective transport along with water. Staverman introduced the reflection coefficient, σ, to relate the observed osmotic pressure gradient (Δ Πobs) to the ideal osmotic pressure gradient (ΔΠideal) with an impermeable solute34: Embedded Image Embedded Image σ is a dimensionless number between 0 and 1. In the presence of a concentration gradient, a solute with σ = 1 produces maximal osmosis, whereas a solute with σ = 0 fails to generate osmotic water movement. Why is there no net water flux when σ = 0 despite a large concentration gradient (Δ[c] ≫ 0)? Qualitatively, solute movement down its concentration gradient provides free energy for water to move against its osmotic gradient, which counteracts favorable water movement in the opposite direction; thus, no net water transport results. For solute to provide free energy for uphill water movement, both solute and water must move in a coupled fashion along the same pathway.35

Solutes are often classified as effective or ineffective osmoles on the basis of their ability to generate osmotic water movement. Osmotic water flux requires a solute concentration gradient (Δ[c] ≫ 0) and a sizeable reflection coefficient (σ ≫ 0); effective osmoles satisfy both requirements, whereas ineffective osmoles fail on one or both counts. Simplistically, a solute concentration gradient across a membrane barrier must be generated faster than the solute flux across the barrier to sustain a concentration gradient for osmosis. Flux of noncharged solutes is often divided into a diffusive component in the absence of water movement and a convective component, which occurs with water flux (Jv)33,36: Embedded Image where Js is solute flux, PD is diffusive permeability, and [c]m is mean solute concentration across the membrane. Generally, solutes with low reflection coefficients (σ → 0) have high PD, because the transport pathway allowing for convective solute transport with water also typically supports diffusive solute movement.37 The converse is not necessarily true because solutes with high reflection coefficients (σ → 1) may or may not exhibit low PD depending on the characteristics of the independent pathway for solute diffusion. If the independent pathway is simple diffusion through the lipid bilayer, PD is typically low.38 Alternatively, if the independent pathway is transporter facilitated, PD is relatively high. For example, along inner medullary collecting ducts, urea and water move independently through urea transporter and aquaporin facilitated pathways during high ADH states; thus, PD is high, but σ is near 1.39,40

Urea illustrates the relationship between solute osmotic efficacy and membrane permeability, diffusion surface area, and kinetics of solute generation or loss. Urea is relatively hydrophilic and exhibits low PD (∼ 1 to 5 × 10−6 cm/s) and a reflection coefficient near 1 in artificial lipid bilayers; thus, urea is quite effective at eliciting osmosis when urea concentration gradients are abruptly created in these experimental systems.41 This is in contrast to ethanol, which exhibits high PD (∼ 10−4 cm/s) even with model membranes.42 However, textbooks often suggest that urea is freely diffusible across membranes and therefore an ineffective osmole.3 Urea transporters facilitate urea diffusion across some biologic membranes, but even the low permeability of pure lipid bilayers is sufficient to minimize urea concentration gradients as total cell membrane surface area is quite large (∼1.2 × 108 cm2 or 12,000 m2), and urea generation rate is comparatively meager.43 Even if one assumes a robust urea generation rate of about 40 g/d, pure lipid bilayer permeability, and no urea excretion or metabolism, a transcellular urea gradient of only about 0.025 mM is expected (see supplemental material). Transcapillary urea gradients are also minimal for most capillary beds as a result of high diffusive permeability and a low reflection coefficient (σ < 0.1) with urea easily traversing interendothelial pores.44–46 However, cerebral capillaries exhibit low urea permeability with a sizeable reflection coefficient of about 0.5.47,48 If blood urea nitrogen falls at a rate of 50 mg/dl/h during hemodialysis, a cerebral transcapillary urea gradient of about 0.25 mM can develop leading to a ∼2.4 mmHg (19.34 * 0.5 * 0.25 mOsm/kg) osmotic pressure favoring capillary filtration (see supplemental material). Cerebral interstitial edema or dialysis disequilibrium may ensue from such a rapid fall in blood urea nitrogen.49

At the capillary-interstitial interface, plasma proteins are excluded from interendothelial pores and act as effective osmoles, whereas small solutes such as Na+, Cl−, and urea are ineffective osmoles freely moving across interendothelial spaces.44,50 Oncotic and colloid osmotic pressure (COP) are used to describe protein-generated osmotic pressure, but these terms unfortunately suggest a distinct mechanism. Oncotic pressure or COP are simply osmotic pressures with protein colloids as effective osmoles and smaller solutes as ineffective osmoles. We will use COP only as shorthand to specify measurement technique rather than biologic phenomena. Oncometers consisting of membranes impervious to protein but permeable to small solutes only measure COP as opposed to osmolality measurements on the basis of freezing point or vapor pressure, which measure osmotic pressure related to all dissolved molecules including small solutes and proteins.51 Charged proteins generate COP not only as dissolved molecules but also through electrostatic attraction of oppositely charged small counter-ions known as a Donnan effect.52 Protein charge depends on isoelectric point (pI); if pH is greater than pI, the protein is negatively charged and vice versa. Plasma albumin with a pI = ∼5 is quite negatively charged at physiologic pH and attracts Na+ and other cations. Thus, albumin COP (approximately 16 to 18 mmHg) relates to its relatively high plasma concentration and low molecular weight (4 g/dl ≅ 0.58 mmol/kg = 11.2 mmHg; effective molecular weight [MW] = 69 kD) and electrostatically associated cations (17 mmHg − 11.2 mmHg = 5.8 mmHg; ∼33% of albumin COP).45

Plasma COP is often equated with albumin COP. Although albumin is the largest single contributor to plasma COP accounting for about 66% to 75% of the normal COP (albumin COP ≅ 16 to 18 mmHg, normal plasma COP ≅ 25 mmHg), the remaining nonalbumin plasma proteins or globulins should not be ignored particularly in hypoalbuminemic states.45 The osmotic efficiency or the osmotic pressure per g/dL of protein can be grossly examined using an average molecular weight (MWav; see supplemental material): Embedded Image Proteins with higher MWav are less osmotically efficient for each g/dl of protein and vice versa. Plasma albumin exhibits a MWav of ∼69 kD, whereas plasma globulins are less efficient with a MWav of ∼150 kD (see supplemental material).45,53–55 Additionally, globulin fractions exhibit different MWav: α1 = 45 kD, α2 = 115 kD, β = 125 kD, and γ = 145 kD.56,57

Analbuminemia or congenital absence of albumin is a rare human disorder (approximately 40 to 50 reported cases worldwide) recapitulated in Nagase mutant rats, which surprisingly exhibit only mild edema and low normal BP.58–60 Plasma COP is partially maintained (approximately 50 to 60% normal) by maintaining total protein concentration in the 5 to 6 g/dl range. Equally important, the relative contributions of α and β fractions, particularly α1, increase relative to γ globulin, increasing the osmotic efficiency of globulins with a fall in MWav from ∼150 kD to ∼113 kD (see supplemental material).45,58,59 Analbuminemic rats preserve plasma COP even more efficiently with near normal levels using a reduction in globulin MWav from approximately 150 to ∼97 kD (see supplemental material).61

Inflammatory hypoalbuminemia behaves similarly as α1 and α2 fractions—the acute phase proteins—dramatically rise, but differs compared to analbuminemia because the β fraction is unchanged, and the γ fraction often increases with chronicity.62 Thus, globulin osmotic efficiency improves but slightly less compared with analbuminemia with a fall in globulin MWav from ∼150 kD to ∼120 kD (see supplemental material). The larger osmotic contribution of plasma globulins facilitates a robust defense of plasma COP in inflammatory states and explains the superior correlation of plasma total protein concentration with plasma COP compared with serum albumin in critically ill, hypoalbuminemic patients.63,64

Cell Volume Homeostasis and Body Na+ and K+ Distribution

Tonicity is shorthand for the action of effective osmolality across a barrier and in this context traditionally refers to the volume behavior of cells in a solution. Osmolality, on the other hand, measures both effective and ineffective osmoles in a kilogram of body fluid.65–67 For instance, ethanol elevates plasma osmolality but does not affect tonicity by rapidly permeating lipid bilayers. Thus, estimates of tonicity are physiologically relevant, whereas osmolality is an imperfect surrogate for tonicity and requires appropriate discounting of ineffective osmoles.65–67

The relative abundance of effective osmoles in intracellular and extracellular compartments dictates body water distribution between ICF and ECF. All cells contain largely fixed or poorly permeable anions such as metabolites (ATP, phosphocreatine, and sulfate), nucleotides, and proteins.29 K+ acts as the primary counter-ion and serves optimal ribosomal protein synthesis requiring high intracellular K+ concentrations.68 The fixed intracellular anions and K+ counter-ions create a Donnan effect-related osmotic gradient favoring persistent water entry. To counteract this osmotic gradient, Na+-K+-ATPases actively extrude Na+ ions producing a double-Donnan effect.69 Cl− passively moves with Na+ to maintain electroneutrality leading to osmotic equilibrium (Figure 2).29,70 In essence, the cells expend ATP to convert permeable Na+ and K+ ions into impermeable, effective osmoles sequestered in the ECF and ICF, respectively. Similarly, Cl− concentrates in the ECF, whereas fixed anions predominate in the ICF.29,71 Water passively distributes into the ECF or ICF compartments in proportion to the effective Na+ and K+ content to reach effective osmotic equilibrium (tonicity) and establish cell volume.

Figure 2.
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Figure 2.

Double-Donnan effect and cell volume homeostasis. (A) Fixed intracellular anions (A−) create a large Donnan effect-related osmotic pressure favoring untenable water entry. (B) The Na+/K+ ATPase essentially fixes Na+ ions extracellularly to create a Na+-related Donnan effect. The Na+ and fixed anion effects counteract one another to form a double-Donnan steady state with no transmembrane osmotic gradient. Adapted from reference 29.

Almost 98% of total body Na+ is distributed among the ECF subcompartments (Table 4). Total body Na+ is often divided into exchangeable (Na+ex) and nonexchangeable domains on the basis of the extent of radioisotope Na+ equilibration with the body pool. About 20 to 30% of total body Na+ is nonexchangeable, residing in anhydrous bone matrix.21,72–74 Total body K+ diametrically mirrors Na+ with about 95% located intracellularly (Table 4). Unlike Na+, however, over 90% of body K+ is exchangeable.75 Total body K+ is proportional to cell mass and reflects metabolic activity. Red blood cell volume supplies oxygen for tissue metabolism and expectedly correlates with total body K+.76 Chronic diseases with wasting or inflammation demonstrate proportionate declines in body cell mass, total body K+, and red blood cell volume.4

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Table 4.

Body sodium and potassium distribution

Extracellular Fluid Dynamics

New studies suggest that the plasma and lymphatic compartments and the interstitium are dynamic interfaces for water distribution. ECF is heterogeneous, yet plasma, representing approximately 17% of ECF, is used to assess body fluid homeostasis. Neurohormonal and renal homeostatic mechanisms sense and defend effective circulating blood volume, a poorly measurable quality of arterial filling determined primarily by blood volume, cardiac output, and vascular tone.77 The kidney primarily modifies sodium balance in response to effective circulating blood volume derangements.78–80 Although the kidney's set point focuses on the vascular compartment, its homeostatic response affects the entire ECF as sodium distributes throughout this compartment. Thus, only the vascular compartment is actively regulated, whereas homeostasis of the significantly larger extravascular ECF, particularly the interstitium, depends primarily on local autoregulation.80,81

Unlike the tonicity equilibrium between cells and their surrounding environment, plasma volume is maintained as a nonequilibrium steady state with net water loss into interstitium and an equivalent water gain caused by lymphatic drainage from interstitium back into plasma. About 8 L/d of capillary filtrate moves into lymphatics with about 4 L reabsorbed in lymph nodes and the remainder returning to the circulation through the thoracic duct.82 Although capillary filtration has historically dominated investigation, interstitial balance equally depends on lymphatic function.81 The modified Starling relationship mathematically delineates water flux between capillary plasma and interstitium36,45,83: Embedded Image where Lp is hydraulic permeability, Pc and Pi are capillary and interstitial hydrostatic pressures, Πc and Πi are capillary and interstitial colloid osmotic pressures, and σ represents the osmotic reflection coefficient. At steady state, capillary filtration must equal lymphatic flow. The magnitude of hydrostatic and osmotic pressures and their balance across capillaries has been debated continuously over the last century. Physiology textbooks still present a framework where filtration predominates at the arterial end and reabsorption occurs at the venous end of the capillary with falling capillary hydrostatic pressure and constant capillary osmotic pressure (Figure 3).3,45 Recent studies, however, point to low net filtration with net filtration pressures (Jv/Lp) of 0.5 to 1 mmHg across the entire length of the capillary in most vascular beds (Figure 3).84 A better understanding of interstitial matrix and reassessment of Πi and Pi has driven this paradigm shift.

Figure 3.
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Figure 3.

Paradigm shift in transcapillary fluid exchange. (A and B) Classic view of transcapillary Starling forces with Πi and Pi ignored leading to predominant filtration on the arterial end giving way to absorption on the venous end. (C and D) Πi is a nonlinear function of filtration rate (Jv). Filtration rate is the intersection of this function and Πi as a function of Starling forces. The Starling relationship is linear with a slope equal to 1/σKf and y intercept of Πc + Pi/σ − Pc/σ. As Pc falls along the capillary, the linear Starling curve left shifts as the y intercept increases. The left shift is blunted by a decrease in Pi. Πi increases with falling filtration, and the relative steepness of the nonlinear Πi (Jv) function maintains filtration along the capillary.

Interstitial and connective tissues are typically modeled as triphasic systems85: free flowing fluid with albumin, a gel phase with glycosaminoglycans (GAGs), and a collagen-based matrix (Figure 4). Water and small solutes (Na+, Cl−, and urea) move easily between all compartments according to prevailing osmotic, hydrostatic, and electrochemical forces.86 Albumin is excluded from the GAG compartment, and both GAGs and albumin are excluded from the collagen matrix.87,88 GAGs generate osmotic pressure (ΠGAG) both in proportion to concentration and negative charge density as the latter attracts cations and increases tissue Na+ concentration.85,89 The collagen matrix generates a hydrostatic pressure that typically opposes GAG osmotic swelling (Pcollagen).90,91 Except for hyaluronan, most GAGs are proteoglycans consisting of a carbohydrate GAG attached to a core protein that interacts with the collagen matrix.92 Measured Pi reflects a balance between matrix hydrostatic and osmotic pressures (Figure 4)93,94: Embedded Image Πi is primarily related to interstitial albumin concentration. The relative rates of water and albumin flux determine steady-state interstitial albumin concentration. Classically, albumin flux was assumed trivial compared with water flux with σ ≅ 1 and interstitial albumin concentration and Πi ≅ 0. However, approximately 50 to 60% of albumin content resides in the extravascular compartment at a concentration of about 1 to 1.5 g/dl with 10 g of albumin moving from plasma to lymph per hour.82 Because GAGs and collagen exclude albumin from about 25 to 50% of the interstitial volume,88,95 the effective albumin concentration in the interstitium approaches 2 to 3 g/dl. Πi is 30 to 60% of Πp when directly measured and mirrors effective albumin concentration. Filtration rate dynamically regulates Πi with increased filtration diluting interstitial albumin and lowering Πi and absorption raising Πi.96 Πi changes steeply with filtration rate as water flux outstrips albumin flux, and albumin exclusion falls producing a disproportionate decline in effective albumin concentration compared with dilution alone.88

Figure 4.
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Figure 4.

Triphasic interstitial model. Schematic representation of interstitial elements and their relationship to capillary forces and lymphatics. Interstitial Pi is a balance between GAG osmotic pressure (ΠGAG) and collagen hydrostatic pressure (Pcollagen). Net capillary filtration (JV) must equal lymphatic flow (JL) at steady state.

Interstitial protein gradients produce an even steeper nonlinear fall in Πi with filtration rate. In a two-pore model, most filtration occurs through small pores with σalbumin ≅ 0.95, whereas large pores transport a minute fraction of water but allow for significant albumin convection with large pore σalbumin ≅ 0.05.97 Effective albumin concentration in close proximity to small pores determines filtration as albumin infusion into the general pericapillary interstitium minimally affects filtration.98,99 Albumin gradients develop with high concentrations around large pores and low concentrations near small pores (Figure 5).100 Whether diffusion between pore regions is limited enough to sustain substantial protein concentration gradients is unclear. A variation of the theme proposes endothelial glycocalyx as the primary interendothelial, small pore permeability barrier with subglycocalyx Π as the primary determinant of filtration; restricted access to the subglycocalyx space may further limit albumin diffusion to amplify interstitial concentration gradients.101

Figure 5.
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Figure 5.

Effect of local interstitial protein gradients on Πi. (A) Schematic of two pore model where albumin primarily moves through large pores leading to high albumin concentration near large pores and low concentration near small pores. Local interstitial osmotic pressure will primarily reflect osmotic pressure in the vicinity of the small pore, which is lower than bulk osmotic pressure. Increasing filtration rate will more steeply decrease local osmotic pressure as albumin wash-out increases around the small pore. (B) Qualitative interstitial osmotic pressure (Πi) versus filtration rate (Jv) curves. When Πi equals bulk Πi, no interstitial protein concentration gradients exist leading to higher Πi, and shallower dependence on Jv as albumin “washout” is less effective. When Πi reflects local Πi with a significant protein gradient between small and large pores, the effective Πi is lower and more steeply dependent on Jv, because albumin washout occurs more efficiently in the vicinity of small pores.

Interstitial hydrostatic pressure (Pi) is slightly negative to zero (−4 to 0 mmHg).93,96,102,103 At euvolemia, the interstitium behaves as a low compliance system; small increases in interstitial volume or capillary filtration rate are met with a steep increase in Pi which counteracts capillary filtration and edema. However, once interstitial volume rises by approximately 20 to 50% or Pi is slightly positive, the interstitium becomes highly compliant. Large changes in volume now minimally increase Pi leading to unabated capillary filtration and edema (Figure 6).104,105 Interstitial cells dynamically regulate Pi shifting the entire Pi-interstitial volume curve. Cells loosen or tighten their grip on collagen matrix through cell surface integrin receptors, which interact with the extracellular matrix and the force-generating actin cytoskeleton.106,107 Reducing collagen β1-integrin receptor interactions and/or cytoskeletal depolymerization dramatically shifts the Pi-volume curve rightward (Figure 6). Inflammatory states and thermal injury decrease Pi as a result of reduced integrin binding of collagen and/or thermal denaturation of collagen.108–110 A fall in Pi may play a critical role in the early phase of sepsis- and burn-induced edema.111,112 Pi eventually rises with interstitial fluid accumulation, whereas increased Pc and Lp and diminished σ maintain edema in the long term (Figure 6).107

Figure 6.
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Figure 6.

Dynamics of measured interstitial hydrostatic pressure Pi. (A) Pi normally varies with interstitial volume (IFV). At low IFV, compliance is low, and pressure rises significantly. Once IFV increases 20 to 50% above euvolemia, compliance increases dramatically, and Pi essentially remains near constant allowing for edema formation. (B) Pi reflects a balance between Pcollagen and ΠGAG. Release of integrin-mediated tension on collagen matrix decreases Pcollagen and right shifts the Pi-IFV curve, leading to increased filtration until Pi rises with edema.

Dynamic interstitial forces play a critical part in regulating plasma-interstitial fluid balance in pathologic states. In hypovolemia, Pc falls in vasoconstricted vascular beds leading to transient interstitial fluid absorption in a process known as transcapillary refill. Subsequent loss of interstitial volume concentrates albumin, increases Πi, decreases Pi, and thus limits transcapillary refill at approximately 75 to 80% of lost plasma volume (Figure 7).105,113,114

Figure 7.
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Figure 7.

Transcapillary dynamics in hypovolemia and nephrotic syndrome. (A) Vasoconstriction with hypovolemia decreases Pc and left shifts the Starling Πi curve with an increased y intercept. Transient interstitial fluid absorption occurs increasing Πi and restoring a lower level of steady-state filtration. Pi also decreases leading to a small right shift in linear Starling relationship (not shown). Transcapillary refill of plasma volume occurs with absorption but may continue to occur if lymphatic flow is slow to match the lower steady-state filtration rate. (B) Πc falls in nephrotic syndrome leading to a right shift of the Πi Starling curve and transiently increased filtration. The subsequent fall in Πi reduces filtration to a new steady state. Pi would increase slightly producing a left shift of the Starling linear curve to further minimize a rise in filtration (not shown).

Nephrotic syndrome demonstrates the interplay between serum albumin, Πc, capillary filtration, and Πi. Unlike other hypoalbuminemic states, in nephrotic syndrome the globulin MWav rises significantly to ∼215 kD because of a preferential urinary loss of osmotically efficient low molecular weight proteins along with albumin and an accumulation of high molecular proteins such as α2-macroglobulin, fibrinogen, haptoglobin multimers, and β lipoproteins (see supplemental material).61,62,115,116 Thus, nephrotic patients are unable to defend Πc despite a reasonable serum total protein concentration as the globulin fraction consists of large, osmotically inefficient proteins. Πc declines proportionately with falling plasma albumin as opposed to analbuminemic patients who exhibit approximately 50% of normal Πc without plasma albumin. Capillary filtration rises with falling Πc, but the subsequent fall in Πi and rise in Pi tends to normalize filtration and minimize edema (Figure 7). The difference between Πc and Πi remains constant at about 50% of Πi or about 12 mmHg, essentially negating the tendency for low Πc to produce edema.117,118 Of course, because the floor for Πi is zero, once Πc falls below 10 to 14 mmHg (around 1.5 to 2 g/dl plasma albumin), edema is inevitable.117,119 A rapid fall in Πc as seen in pediatric nephrotic crises with minimal change disease may kinetically outstrip the compensatory fall in Πi, leading to intravascular volume depletion and hemodynamic compromise.120–122

Whether a fall in Πc is the primary determinant of nephrotic edema is controversial, because several investigators have demonstrated autonomously enhanced sodium reabsorption in the distal nephron and diminished sodium excretion preceding hypoproteinemia.123–126 Proponents of the overfill hypothesis suggest that renal sodium retention is the primary abnormality in nephrotic syndrome, whereas others support the underfill theory wherein low Πc leads to intravascular volume depletion and secondary renal sodium retention.127,128 Increased capillary permeability measured as albumin extravasation (increased Lp and/or decreased σ) may also contribute to intravascular volume depletion.129 As in all controversies, individual patients may demonstrate overfill, underfill, or mixed physiology. Severe or rapidly developing hypoproteinemia and clinical features of volume depletion support underfilling, whereas hypertension, renal dysfunction, and mild to moderate hypoalbuminemia (serum albumin >2 g/dl) suggest overfilling.128,130 Minimal change disease more commonly presents with underfill physiology, but whether histologic diagnosis is an independent predictor of volume homeostasis or simply reflects more severe or rapid hypoproteinemia with maintained GFR is unclear.131

Total Body and Compartmental Tonicity

Animal cells are largely in osmotic equilibrium with their surrounding environment; thus, intracellular tonicity equals interstitial tonicity.30,132,133 Clinicians estimate plasma tonicity, which may not equal interstitial and intracellular tonicity except in the case of red blood cells (ΠRBC = Πplasma). Fortunately, in most body tissues, the difference between plasma and interstitial osmolality is minimal. A direct experimental measurement of the plasma to interstitial osmolality gradient is the difference in COP, which accounts for both protein and Donnan small ion effects96: Embedded Image The tonicity gradient between plasma and interstitium is quite small, and we can hypothesize that tonicity is equal across all body compartments: Embedded Image In addition, if we assume the vast majority of effective body osmoles are exchangeable Na+ and K+ and their counter-ions and glucose, the following idealized relationship is derived (see supplemental material): Embedded Image where fPW is the plasma water fraction, and GNa is a correction factor for hyperglycemia related translocational hyponatremia that has been proposed to range from 1.5 to 2.4 mEq/L per 100 mg/dl rise in plasma glucose.134–136

Studies examining the relationship between exchangeable Na+ and K+, TBW, and PNa and PK in normal and pathologic states have found that PNa may be delineated as follows (see supplemental material): Embedded Image Because total exchangeable cation (Na+ex + K+ex) is on the order of 90 mEq/kg, the ideal relationship is a good approximation because 250 mEq pales in comparison and 1.03 is also quite close to 1. The 250 mEq deviation from ideality relates to a small osmotic gradient between plasma and total body osmolality, non-Na+ and K+ osmoles besides glucose, and exchangeable excess Na+ and K+ (see supplemental material). The nonideal quantity of 250 mEq in the derived PNa relationship applies only to well represented pathologic states within patient cohort data (congestive heart failure, cirrhosis, and low Na+ diet). Whether this quantity is similar in other disease states such as syndrome of inappropriate anti-diuretic hormone (SIADH), volume depletion, and high Na+ diet is unknown and potentially limits the accuracy of the idealized PNa approximation in these situations.137

Broadly speaking, excess Na+ and K+ refer to any compartment where exchangeable Na+ and K+ concentration exceeds plasma water [Na+ + K+]. Often, excess Na+ and K+ is used interchangeably with osmotically inactive, although the two are not mechanistically synonomous. Some inaccurately suggest that a compartment with osmotically active Na+ and K+ in excess of plasma should accrue water until equilibrium with plasma is reached and excess cation is eliminated; thus, the persistence of a concentration gradient can only occur if excess cation is osmotically inactive. But excess cation may be osmotically active while maintaining a concentration gradient in two ways: a counteracting hydrostatic pressure balances the osmotic gradient, allowing compartment osmolality to differ from plasma osmolality or excess Na+ and K+ is counterbalanced by loss of non-Na+ and K+ osmolytes maintaining plasma and total body tonicity. Of course, a portion of excess Na+ and K+ may truly be osmotically inactive (ϕ = 0). Bone and a small fraction of intracellular cation probably represent the osmotically inactive pool. Some may argue that the distinction between excess and osmotically inactive cation is pure semantics, but a growing literature on cartilage suggests otherwise. Cartilage is hypertonic, yet inexorable cartilage swelling is prevented by a counteracting collagen-based hydrostatic pressure.91,138

Significant attention has historically focused on the dynamics of Na+ balance given its prominent role in hypertension and edematous states. When positive Na+ balance occurs, Na+ is handled in several ways: Na+ accumulates in the extracellular space with water such that plasma tonicity and Na+ concentration remain constant; Na+ is retained in excess of water with a resulting increase in plasma Na+ concentration and tonicity along with a parallel rise in total body tonicity; or Na+ is retained in excess of water with little or no change in plasma tonicity often termed excess Na+ storage (or inaccurately as osmotically inactive Na+ storage). Broadly speaking, several mechanisms account for excess Na+ storage: negative K+ balance offsets positive Na+ balance such that total cation balance is unchanged; positive total cation balance (Na+ + K+) with negative balance for effective osmoles other than Na+ or K+ salts such that total body effective osmoles remain constant; positive cation balance with osmotically active Na+ associating with interstitial glycosaminoglycans, thereby increasing total body tonicity relative to plasma; or positive cation balance with osmotically inactive Na+ associating with bone mineral matrix or possibly intracellular proteins (Figure 8).

Figure 8.
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Figure 8.

Mechanisms of excess sodium storage. (1) Na+ may be exchanged for K+, and the latter is excreted. Because total cation content is unchanged, both cation content and tonicity remain constant. (2) Positive Na+ balance is matched by negative osmolyte (O) balance. (2a) O may be cationic (O+; e.g. choline) and undergo exchange with Na+. (2b) O may be a zwitterion (e.g. taurine and amino acids) or uncharged (e.g. sorbitol and inositol) and essentially exchange for NaCl. In both cases, cation content rises, but total solute content is unchanged. (3) Intracellular anions (A−; e.g. sulfate) may be exchanged with chloride and incorporated into GAGs. Na+ associates with GAG sulfate moieties via electrostatic interactions. Both total cation and solute content rise in this situation. Tonicity may or may not rise depending on the extent of osmole efficacy. For instance, Na+ salts with bone mineral matrix and possibly intracellular anions may be osmotically inactive; in this case, total body tonicity is unchanged.

Although measuring K+ balance is straightforward, defining other mechanisms for excess Na+ storage is difficult. Over the last half century, investigators have debated whether the movement of Na+ ions into or out of a storage compartment is necessary to account for positive sodium balance with high sodium diet and edematous states and negative sodium balance in volume depletion or hypotonicity. Many studies neglect K+ balance, which can often offset a positive Na+ balance.139–143 Even after accounting for K + balance, most short term metabolic balance studies (3 to 5 days) and long term (1 to 3 months) radioisotopic studies generally find reasonable correlation between cation and water balance and plasma tonicity,144–149 whereas intermediate balance studies (7 to 14 days) often suggest Na+ storage.150–152 Whether these differences reflect biologic phenomena or technical differences remains unclear.150,153–155

Excess Na+ storage occurs with high sodium diet (>300 mEq/d). Some excess Na+ exchanges with intracellular K+ primarily from skeletal and vascular smooth muscle; thus, total cation balance in this case is unchanged.147,156 Excess Na+ storage in skin exceeds negative K+ balance and has recently garnered attention, although the hypothesis dates back over 30 years in the Russian literature.150,157,158 Animal studies demonstrate increased Na+ concentration in skin (20 to 40 mEq/L tissue water), which translates to about 1 to 2 mEq/kg excess Na+ storage, assuming that skin water is at most 5% of body weight.27,156,159 Excess Na+ may accumulate intracellularly in exchange for non-K+ osmolytes or alternatively associate with interstitial glycosaminoglycans (Figure 8). Radiotracer Na+ dynamics in isolated skin from animals on high sodium diets suggest an increase in the rapidly exchanging extracellular Na+ pool rather than the more slowly exchanging intracellular Na+ pool. Within the rapidly exchanging pool, a compartment outside the inulin space accounts for the majority of the increased Na+ content, suggesting a sterically inaccessible site such as interstitial glycosaminoglycans.157 Indeed, negatively charged, sulfated glycosaminoglycan content rises in skin with dietary Na+ loading.159–161 Na+ loading transiently increases interstitial flow and possibly Na+ concentration, which are known stimulators of interstitial cell matrix production, particularly sulfated GAGs.162–165

Na+ associated with negatively charged purified glycosaminoglycans in cartilage exhibits an osmotic coefficient similar to normal saline; thus, skin GAG-associated Na+ is most likely osmotically active.86,166 Assuming that 20 to 40 mEq/L Na+ is stored in association with skin interstitial GAGs, ΠGAG would rise about 100 to 200 mmHg or approximately 5 to 10 mOsm/kg to achieve the required negative charge density (see supplemental material). Typical transcapillary Starling forces pale in comparison with 100 to 200 mmHg. Dermal swelling pressures can reach up to 100 to 150 mmHg in situations where Pcollagen is reduced, suggesting that ΠGAG is normally quite high but counteracted by Pcollagen.111,167 Thus, ΠGAG can rise significantly but requires similar increases in Pcollagen to prevent high filtration rates and interstitial edema caused by low Pi (Pi ∝ Pcollagen − ΠGAG). Hydrostatic pressure (Pcollagen) counterbalances osmotic pressure (ΠGAG) to eliminate water movement and maintain a small tonicity gradient between dermal interstitium and plasma. Dermal fibroblasts like their chondrocyte counterparts probably accommodate interstitial hypertonicity with osmolyte accumulation to maintain cell volume.168,169

When the mechanism of excess Na+ storage across tissues is broadly surveyed, a hypothetical framework comes into view. Relatively cellular tissues such as muscle exchange Na+ for K+ or other intracellular osmolytes as their high cell mass relative to interstitial space provide a large osmole depot for transcellular exchange. Conversely, relatively acellular connective tissues have minimal intracellular osmoles at their disposal and alternatively depend on osmotically active storage with interstitial glycosaminoglycans or osmotically inactive storage with mineral matrix.

The Na+ counter-anion may also modify Na+ storage. When subjects consume large amounts of Na+ either as Cl− or bicarbonate (or equivalents such as citrate or ascorbate) salts, hypertension and plasma volume expansion ensues only with NaCl intake despite equivalent positive sodium balance and weight gain with sodium bicarbonate. Furthermore, NaCl consumption results in hypercalciuria, whereas sodium bicarbonate does not change urinary calcium excretion.170–173 Taken together, water distribution tends to be extravascular and does not affect calcium homeostasis when the Na+ counter-anion is bicarbonate. Na+ with a base equivalent may possess a larger pool of intracellular and bone storage mechanisms. Intracellular proteins can simply titrate bicarbonate with protons with the resulting protein anionic side chain acting as a counter-ion for excess Na+ in a potentially osmotically inactive form. For NaCl to store Na+ in association with intracellular proteins, Na+ would have to displace protein side chain H+ or exchange with predominantly protein bound Ca2+. The former is unlikely because cells function poorly with intracellular acidosis, whereas the latter necessitates Ca2+ excretion. The low solubility of calcium bicarbonate probably precludes intracellular Na+/Ca2+ exchange with sodium bicarbonate but promotes bone surface crystal integration of sodium bicarbonate in toto. Conversely, NaCl requires Na+/Ca2+ exchange at the bone matrix interface again leading to hypercalciuria.174–176 Thus, negative Ca2+ balance potentially limits Na+ storage in the setting of high NaCl intake, but not with bicarbonate salts. Although purely speculative, these hypotheses provide fertile ground for future investigation.

Alterations in intracellular, skin, and bone Na+ storage may critically regulate blood volume homeostasis and participate in the pathogenesis of salt-sensitive hypertension. These storage mechanisms may buffer the blood volume against transient or sustained sodium loads. Animals and patients with reduced Na+ storage capacity are prone to blood volume expansion and hypertension.158,177,178 Alternatively, these storage mechanisms activate deleterious neurohormonal and/or inflammatory signaling pathways.159 Although this work points to an exciting paradigm shift in blood volume regulation, whether these mechanisms contribute to pathology broadly or in a narrow subset of patients remains unknown. Because United States dietary Na+ intake is 150 to 200 mEq/d ± 100 mEq/d (2 SD)179–181 and storage mechanisms regulating Na+ homeostasis require dietary intakes exceeding 300 mEq/d,140,150 only about 5% of essential hypertension in American patients may involve alterations in Na+ storage.

Conclusions

Understanding body fluid dynamics is critical to the practice of medicine. Phenomenal work accomplished during the last century has lulled us into relying on aging textbook dogma or believing there is little left to discover. However, re-examination of foundational literature suggests some teachings stray from original data. The division of TBW into ICF and ECF is frequently taught as an arbitrary distribution rather than a product of cell volume homeostasis and the relative partitioning of body fat, protein, Na+, and K+. New investigations also suggest novel paradigms involving the dynamic nature of the interstitium that critically regulate ECF homeostasis. Although diet and the kidneys arbitrate blood volume homeostasis in the long run, the interstitium plays a larger role in short term blood and interstitial volume adjustments. Short term Na+ storage and interstitial volume homeostasis may be relevant to transient or nonequilibrium phenomena such as BP dipping, flash pulmonary edema, rapid blood loss, burns, and sepsis, to name a few. Future investigation will hopefully unify the molecular and structural biology of interstitial cell-matrix interactions with classic Starling physiology to identify new therapeutic targets for hemodynamic derangements.

DISCLOSURES

None.

Acknowledgments

The authors thank Raymond Harris, Sanjeev Shah, Peter Aronson, and Roland Blantz for comments on earlier versions of this manuscript and Sergei Chetyrkin for translation of the references in Russian.

Footnotes

  • Published online ahead of print. Publication date available at www.jasn.org.

  • Supplemental information for this article is available online at http://www.jasn.org/.

  • Copyright © 2011 by the American Society of Nephrology

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Journal of the American Society of Nephrology: 22 (12)
Journal of the American Society of Nephrology
Vol. 22, Issue 12
1 Dec 2011
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Body Fluid Dynamics: Back to the Future
Gautam Bhave, Eric G. Neilson
JASN Dec 2011, 22 (12) 2166-2181; DOI: 10.1681/ASN.2011080865

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Body Fluid Dynamics: Back to the Future
Gautam Bhave, Eric G. Neilson
JASN Dec 2011, 22 (12) 2166-2181; DOI: 10.1681/ASN.2011080865
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