On the cover: Large-scale three-dimensional multi-fluorescence imaging of a human nephrectomy section (approximately 7x3x0.05 mm3, left), and tissue cytometry analysis (middle and right) using Volumetric Tissue Exploration and Analysis software (VTEA). Gates were drawn on the scatter plot (upper right) to identify CD68+ (macrophages) and MPO+ cells (neutrophils). The gated cells were mapped to the original volume to identify their spatial distribution (middle). A 3D rendered sub-volume demonstrates the resolution and precision of gating (lower right, gated cells identified by nuclear overlays). Myeloperoxidase (MPO); Tamm-Horsfall protein (THP); Aquaporin-1 (AQP1). Scale bar=500µm. Cover image supplied by Seth Winfree, Kenneth W. Dunn and Tarek M. El-Achkar, Indiana University, Indianapolis, Indiana