PT  - JOURNAL ARTICLE
AU  - Saleem, Moin A.
AU  - O’Hare, Michael J.
AU  - Reiser, Jochen
AU  - Coward, Richard J.
AU  - Inward, Carol D.
AU  - Farren, Timothy
AU  - Xing, Chang Ying
AU  - Ni, Lan
AU  - Mathieson, Peter W.
AU  - Mundel, Peter
TI  - A Conditionally Immortalized Human Podocyte Cell Line Demonstrating Nephrin and Podocin Expression
DP  - 2002 Mar 01
TA  - Journal of the American Society of Nephrology
PG  - 630--638
VI  - 13
IP  - 3
4099  - http://jasn.asnjournals.org/content/13/3/630.short
4100  - http://jasn.asnjournals.org/content/13/3/630.full
SO  - J. Am. Soc. Nephrol.2002 Mar 01; 13
AB  - ABSTRACT. Recent molecular insights have established the podocyte as a key component of the glomerular filtration barrier, and hence an important common pathway in proteinuric diseases. A conditionally immortalized human podocyte cell line has been developed by transfection with the temperature-sensitive SV40-T gene. These cells proliferate at the “permissive” temperature (33°C). After transfer to the “nonpermissive” temperature (37°C), they entered growth arrest and expressed markers of differentiated in vivo podocytes, including the novel podocyte proteins, nephrin, podocin, CD2AP, and synaptopodin, and known molecules of the slit diaphragm ZO-1, α-, β-, and γ-catenin and P-cadherin. The differentiation was accompanied by a growth arrest and the upregulation of cyclin-dependent kinase inhibitors, p27 and p57, as well as cyclin D1, whereas cyclin A was downregulated. These data are consistent with cell cycle protein expression during podocyte maturation in vivo. In conclusion, the development of this cell line provides a new tool in the study of podocyte biology, which will enable accurate assessment of the behavior of these complex cells in health and disease.