Table 1.

Basal luminescence detection of released ATP from cultures or monolayers of ADPKD and non-ADPKD human renal epithelial primary culturesa

ATP Release Assays of Epithelial Cell Cultures Grown on 35-mm Diameter Dishes
Renal Epithelial Cell Cultures Total ATP Release Approximate [ATP]
hPCT1245 ± 87.98 (79)∼500 nM
hCORT828.2 ± 66.62 (30)∼350 nM
hADPKD2592 ± 197.0 (34)b∼2μM
ATP Release Assays of Polarized Epithelial Monolayers Grown on 12-mm Diameter Membrane Supports
Renal Epithelial Monolayers Apical Release Basolateral Release
hPCT19.27 ± 3.15 (16)6.275 ± 1.399 (14)
hCORT10.22 ± 1.74 (17)2.454 ± 0.804 (17)
hADPKD 31.18 ± 3.63 (30)b 9.304 ± 1.736 (28)c
  • a Peak luminescence values in arbitrary light units (ALU) are shown from ATP release assays of confluent cell cultures in 35-mm diameter dishes or ATP release assays from polarized monolayers grown on 12-mm diameter filters. For the latter, both apical and basolateral release values are shown. These luminescence values are total units and are not corrected for volume or for apical versus basolateral membrane estimated surface area. Experimental number (n) is shown in parentheses. Approximate [ATP] concentration relative to a standard curve is also shown (100 nM ATP = 303 ALU; 1 μM ATP = 2184 ALU; and 10 μM ATP = 7259 ALU). Data are expressed as mean ± SEM. ADPKD, autosomal dominant polycystic kidney disease primary cultures; hPCT, human proximal tubule epithelial cell primary cultures; hCORT, human mixed renal cortical epithelial cell primary cultures; hADPKD, human ADPKD epithelial primary cultures.

  • b P < 0.005 by unpaired ANOVA.

  • c P < 0.05 by unpaired ANOVA.