Table 1.

Effects of RU486 on aldosterone- and corticosterone-stimulated Isca

GroupIsc (μA/cm2)
-RU486+RU486
aIsc was measured on confluent mpkCCDc14 cells grown on filters and incubated without (none) or with RU486 (10-6 M) or with aldosterone (10-9 M and 5 × 10-7 M), aldosterone plus a 20-fold excess of RU486, corticosterone (5 × 10-7 M) and corticosterone plus a 20-fold excess of RU486 for 2 h at 37°C. All hormones and compounds were added to both basal and apical sides of the filters. Values are means ± SEM from three to seven experiments.
bP < 0.05 versus untreated cells.
cP < 0.001 versus untreated cells.
dP < 0.001 versus aldosterone- or corticosterone-treated cells.
None8.7 ± 1.57.6 ± 1.2
Aldosterone (10-9 M)15.6 ± 1.5b15.2 ± 2.2
Aldosterone (5 × 10-7 M)22.8 ± 1.3c11.5 ± 1.3d
Corticosterone (5 × 10-7 M)23.8 ± 1.4c13.1 ± 0.6d