Effects of RU486 on aldosterone- and corticosterone-stimulated Isca
| Group | Isc (μA/cm2) | |
|---|---|---|
| -RU486 | +RU486 | |
| aIsc was measured on confluent mpkCCDc14 cells grown on filters and incubated without (none) or with RU486 (10-6 M) or with aldosterone (10-9 M and 5 × 10-7 M), aldosterone plus a 20-fold excess of RU486, corticosterone (5 × 10-7 M) and corticosterone plus a 20-fold excess of RU486 for 2 h at 37°C. All hormones and compounds were added to both basal and apical sides of the filters. Values are means ± SEM from three to seven experiments. | ||
| bP < 0.05 versus untreated cells. | ||
| cP < 0.001 versus untreated cells. | ||
| dP < 0.001 versus aldosterone- or corticosterone-treated cells. | ||
| None | 8.7 ± 1.5 | 7.6 ± 1.2 |
| Aldosterone (10-9 M) | 15.6 ± 1.5b | 15.2 ± 2.2 |
| Aldosterone (5 × 10-7 M) | 22.8 ± 1.3c | 11.5 ± 1.3d |
| Corticosterone (5 × 10-7 M) | 23.8 ± 1.4c | 13.1 ± 0.6d |