Table 1.

Fluorometric AGE assay in glucose-6-phosphate-treated BSAa

CharacteristicBSA ControlAGE-BSAAGE-BSA + AMGD
aData are expressed as mean ± SEM of quadruplicate measurements on 2 mg/ml BSA solutions (wt/vol) at 390/450 nm excitation/emission. AMGD, 250 mM aminoguanidine present throughout the 30-d incubation with glucose-6-phosphate. AGE, advanced glycation end products; BSA, bovine serum albumin.
bP < 0.001 versus BSA control.
Fluorescence units18.2 ± 0.168.9 ± 0.9b21.1 ± 0.5
% of control+277 ± 4+16 ± 3