Table 2.

Effect of 5 day culture of human mesangial cells in NG/HG media on Ca2+ influx and release from intracellular storesa

ConditionMedia Glucose Concentration
5.5 mM30 mM5.5 mM30 mM
+1 mM Aminoguanidine
aData are mean ± SEM nM peak [Ca2+]i from six experiments on independent monolayers equilibrated in Ca2+-free solutions. NG, normal glucose; HG, high glucose; AngII, angiotensin II.
bP < 0.05 versus 5.5 mM glucose.
Baseline63 ± 563 ± 676 ± 567 ± 4
Ca2+ 1 mM263 ± 34146 ± 23b214 ± 27225 ± 35
Ca2+ 10 mM485 ± 85185 ± 22b475 ± 89399 ± 95
+AngII, 1 μM657 ± 45265 ± 27b698 ± 47777 ± 88