Inhibition of apoptosis by caspase inhibitorsa
| Stimuli | Apoptotic Cells (per 100) | ||||
|---|---|---|---|---|---|
| None | z-VAD | DEVD-CHO | 6PTD | ABZ | |
| aPTEC were treated for 24 or 72 h with the indicated stimuli in the absence or presence of the caspase inhibitors z-VAD.fmk (50 μM) and DEVD.CHO (100 μM) and with the immunosuppressors and apoptosis inhibitors 6PTD (0.5 mM) and ABZ (2 mM). The extent of apoptosis was determined by confocal microscopy observation of apoptotic bodies after staining with PI and SYTO13, as described in Figure 4. Results show the mean ± SEM of three experiments (n = 100 to 120 cells). PTEC, proximal tubular epithelial cells; 6PTD, 6(5H)phenanthridinone; ABZ, 3-aminobenzamide; TPP, tris-palmitoyl-peptide; IL-1β, interleukin 1β; GSNO, S-nitrosoglutathione; CsA, cyclosporin A. | |||||
| 24 h | |||||
| none | 2 ± 1 | 1 | 3 ± 2 | 2 ± 1 | 2 ± 1 |
| TPP, 5 μg/ml | 16 ± 1 | 8 ± 1 | 10 ± 1 | 3 ± 1 | 2 ± 1 |
| IL-1β, 20 ng/ml | 11 ± 2 | 8 ± 1 | 7 ± 2 | 3 ± 1 | 4 ± 1 |
| GSNO, 500 μM | 19 ± 2 | 7 ± 1 | 9 ± 1 | 3 ± 1 | 5 ± 1 |
| CsA, 10 nM | 14 ± 2 | 7 ± 2 | 6 ± 1 | 4 ± 1 | 3 ± 2 |
| TPP + IL-1β | 24 ± 3 | 11 ± 2 | 11 ± 2 | 5 ± 1 | 5 ± 1 |
| TPP + CsA | 30 ± 3 | 10 ± 1 | 11 ± 2 | 6 ± 1 | 6 ± 1 |
| GSNO + CsA | 36 ± 4 | 9 ± 2 | 9 ± 2 | 5 ± 1 | 7 ± 1 |
| 72 h | |||||
| none | 3 ± 1 | 4 ± 1 | |||
| GSNO | 25 ± 3 | 12 ± 3 | |||
| CsA | 32 ± 4 | 17 ± 2 | |||
| GSNO + CsA | 52 ± 6 | 19 ± 4 | |||