Table 4.

Quantitative analysis of the expression of FcαR1 expression on U937 and HMC with and without stimulations by IL-1β (4 ng/ml), TGF-β (2 ng/ml), or TNF-α (1000 U/ml) determined by flow cytometry using anti-FcαR1 clone A59a

Cell Type Preincubated with MFI
HMC Isotypic control 6.70 ± 0.17
HMC Plain culture mediumb 6.94 ± 0.35
HMC IL-1β, 4 ng/ml 6.88 ± 0.38
HMC TGF-β, 2 ng/ml 7.00 ± 0.25
HMC TNF-α, 1000 U/ml 6.86 ± 0.15
U937 Isotypic control 3.28 ± 0.30
U937 Plain culture mediumb 10.75 ± 1.13c
U937 IL-1β, 4 ng/ml 11.61 ± 0.85c
U937 TGF-β, 2 ng/ml 9.97 ± 1.15c
U937 TNF-α, 1000 U/ml 21.05 ± 2.79c,d
  • a Results are expressed as mean MFI ± SD of five individual experiments. IL-1β, interleukin-1β; TGF-β, transforming growth factor-β. Other abbreviations as in Tables 123.

  • b Serum-free plain culture medium.

  • c P < 0.01 compared with isotype control.

  • d P < 0.01 compared with medium control.