Table 1.

Binding of PR3 to HUVEC as assessed by FACS analysis using different mAba

mAbIsotypeMFI
PBSPR3
aHUVEC were incubated in the absence or presence of PR3 (20 μg/ml), and binding was detected using different mAb directed against PR3, followed by GAM-PE. mAb anti-MPO served as a control. Binding of the mAb was expressed as mean fluorescence intensity (MFI). PR3, proteinase 3; HUVEC, human umbilical vein endothelial cells; FACS, fluorescence-activated cell sorter; mAb, monoclonal antibody; PBS, phosphate-buffered saline; GAM-PE, phycoerythrin-labeled goat F(ab′)2 anti-mouse Ig; MPO, myeloperoxidase.
mAb-α-PR3
4A5IgG14.767.4
4A3IgG2a4.8127.1
6A6IgG13.044.8
12.8IgG13.961.4
WGM1IgM7.018.1
WGM2IgG13.611.2
mAb-α-MPO
2C7IgG13.64.2
1H2IgG2b4.96.2
5B8IgG18.39.6