Table 2.

Sets of primers used for semiquantitative and quantitative RT-PCRa

PrimerOligonucleotidesAccession No.Expected Size (bp)Annealing TemperatureNo. of PCR Cycles
aThe oligonucleotides were selected from sequences for which the accession numbers are indicated. The forward primer of each set for semiquantitative reverse transcription (RT)-PCR was labeled with the 6-Fam (6-F) dye, except for the tumor necrosis factor-α (TNF-α) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) primers. The size of each amplified sequence, the annealing temperature, and the number of PCR cycles are indicated.
β2-5′-(6-F)GAGATGTCTCGCTCCGTGGCCTTAGC-3′;NM00404837458°C21
Microglobulin5′-CCATGATGCTGCTTACATGTCTCTGATCC-3′
CD35′-(6-F)TCTATAGGTATCTTGAAGGGGCTC-3′;X0393413558°C25
5′-CCAGGCTGATAGTTCGGTGA-3′
IκBα5′-(6-F)TATTTCTCCCTACCAGCTCAC-3′; 5′-M6904356958°C23
CCATGATGCTGCTTACATGTCTCTGATCC-3′
Proteasome α25′-(6-F)CTCAAGGAAAGTCTTCCCATTCAC-3′;D0076045558°C27
subunit5′-TATGCTTTGGCTGCTGTAGCTGGA-3′
GAPDH5′-ACCACAGTCCATGCCATCAC-3′;AF26108545160°C26
5′-TCCACCACCCTGTTGCTGTA-3′
TNF-α5′-ATGGCGTGGAGCTGAGAGATA-3′;AF04334239160°C40
5′-GGGGAGGCGTTTGGGAAGGTT-3′