Table 1. Results of DAF-2 calibrationa

GroupContents050100200400800 nM NOn
a One-way ANOVA analysis showed no significant differences among the seven groups (P > 0.05).
1PBS without Ca2+ and Mg2+20.8 ± 0.350.7 ± 5.460.5 ± 4.190.1 ± 10.5121.8 ± 13.1229.4 ± 15.84
2PBS with 1.3 mM Ca2+20.1 ± 0.751.5 ± 4.761.4 ± 5.991.7 ± 9.6122.8 ± 11.7233.8 ± 19.73
3PBS with 1.3 mM Mg2+21.6 ± 0.652.3 ± 3.261.6 ± 2.494.9 ± 8.8122.5 ± 14.6233.0 ± 20.73
4PBS with 1.3 mM Ca2+ and Mg2+21.3 ± 0.751.8 ± 3.160.8 ± 6.593.7 ± 9.9123.9 ± 13.2233.4 ± 18.24
5PBS with 5 mM EGTA20.5 ± 0.452.1 ± 4.660.7 ± 5.394.3 ± 7.6122.2 ± 12.6232.8 ± 15.14
6Buffer solution with 1.3 mM Ca2+20.6 ± 0.651.4 ± 5.163.7 ± 5.194.4 ± 8.5119.0 ± 13.2229.8 ± 19.83
7Buffer solution with 5 mM EGTA20.0 ± 1.153.8 ± 5.763.2 ± 6.493.2 ± 9.5121.1 ± 14.1230.9 ± 21.33
Total20.8 ± 0.251.9 ± 3.961.5 ± 4.193.2 ± 7.9122.0 ± 9.2231.9 ± 15.624