Table 2. The apoptosis rate in cystinotic nephropathic, variant, and normal fibroblastsa

Cell LinePhenotype% Apoptosis
TNF-αAnti-FasUVControl
a Cells were treated with apoptosis inducers as indicated, stained with CaspACE, and analyzed by fluorescence microscopy. A minimum of 250 cells were scored per condition, and this was done in triplicate, thus at least 750 cells were scored per condition. T-statistics: P < 0.001 for average nephropathic versus average normal; P < 0.05 for ocular versus nephropathic; P > 0.05 for ocular versus normal, intermediate versus nephropathic, and intermediate versus normal.
GM008Nephropathic14.9 ± 0.617.7 ± 2.912.8 ± 4.82.2 ± 0.2
GM760Nephropathic13.5 ± 4.314.4 ± 2.726.3 ± 5.43.1 ± 0.7
GM046Nephropathic16.1 ± 4.122.3 ± 2.913.1 ± 2.02.2 ± 0.6
Average14.818.117.42.5
GM08761Ocular8.2 ± 0.985.9 ± 1.96.4 ± 2.02.3 ± 0.3
GM00379Intermediate11.4 ± 2.07.7 ± 1.08.4 ± 0.943.1 ± 0.2
GM010Normal9.2 ± 1.96.5 ± 2.37.0 ± 0.62.8 ± 0.2
GM05399Normal6.3 ± 1.94.9 ± 0.87.2 ± 1.01.9 ± 0.1
Average7.85.27.12.4