Table 3. The apoptosis rate in normal and cystinotic fibroblastsa

Cell%Apoptosis
PhenotypeTNF-alphaAnti-FasUV
a Cells were treated with apoptosis inducers as indicated, stained with TUNEL, and analyzed by fluorescence microscopy. A minimum of 250 cells were scored per condition, in triplicate. P < 0.05 between all pairs of nephropathic cystinotic cells and the intermediate line, and both normal lines except for those indicated by *, which were not significantly different from either normal line. P > 0.05 for ocular versus normal and intermediate; P = 0.01 for ocular versus nephropathic.
Control
GM00008Nephropathic17.1 ± 2.212.3 ± 1.9*13.7 ± 3.0*3.1 ± 0.5
GM00760Nephropathic19.3 ± 3.417.7 ± 3.321.6 ± 5.82.4 ± 0.3
GM00046Nephropathic15.3 ± 1.1*15.9 ± 3.219.2 ± 2.52.6 ± 0.4
AverageNephropathic17.215.318.22.7
GM08761Ocular5.5 ± 0.87.1 ± 0.86.8 ± 1.93.4 ± 0.8
GM00379Intermediate8.2 ± 1.08.5 ± 3.08.5 ± 3.02.8 ± 0.8
GM00010Normal12.8 ± 2.49.6 ± 2.111.9 ± 1.62.4 ± 0.5
GM05399Normal11.4 ± 1.910.5 ± 2.67.6 ± 0.82.4 ± 0.9
AverageNormal12.110.19.82.4